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Role of IgM specific indirect immunofluorescence assay in diagnosing an outbreak of leptospirosis.
Indian J Pathol Microbiol. 2002 Jan; 45(1):75-7.IJ

Abstract

The study was conducted during a suspected epidemic of leptospirosis in Maharashtra. A total of 13 acute phase blood samples, collected at 5-6 days from the onset of symptoms, and 10 convalescent phase samples, colected at around 20 days from the onset of symptoms were obtained from 13 patients. Sera were separated and the samples were subjected to Microagglutination Test (MAT) and IgM-Indirect Immunofluorescence assay (IFA) to detect antibodies against leptospira. In the acute phase sera, only one sample was positive by MAT while seven were positive by IFA. In the convalescent samples, six were positive by MAT and seven were positive by IFA. IFA is a rapid test and can be used for early diagnosis of leptospirosis.

Authors+Show Affiliations

Department of Microbiology, B J Medical College, Pune.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

12593569

Citation

Joshi, Suvarna, et al. "Role of IgM Specific Indirect Immunofluorescence Assay in Diagnosing an Outbreak of Leptospirosis." Indian Journal of Pathology & Microbiology, vol. 45, no. 1, 2002, pp. 75-7.
Joshi S, Bal A, Bharadwaj R, et al. Role of IgM specific indirect immunofluorescence assay in diagnosing an outbreak of leptospirosis. Indian J Pathol Microbiol. 2002;45(1):75-7.
Joshi, S., Bal, A., Bharadwaj, R., Kumbhar, R., Kagal, A., & Arjunwadkar, V. (2002). Role of IgM specific indirect immunofluorescence assay in diagnosing an outbreak of leptospirosis. Indian Journal of Pathology & Microbiology, 45(1), 75-7.
Joshi S, et al. Role of IgM Specific Indirect Immunofluorescence Assay in Diagnosing an Outbreak of Leptospirosis. Indian J Pathol Microbiol. 2002;45(1):75-7. PubMed PMID: 12593569.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Role of IgM specific indirect immunofluorescence assay in diagnosing an outbreak of leptospirosis. AU - Joshi,Suvarna, AU - Bal,Abhijit, AU - Bharadwaj,Renu, AU - Kumbhar,Rameshwar, AU - Kagal,Anju, AU - Arjunwadkar,Vidya, PY - 2003/2/21/pubmed PY - 2003/3/26/medline PY - 2003/2/21/entrez SP - 75 EP - 7 JF - Indian journal of pathology & microbiology JO - Indian J Pathol Microbiol VL - 45 IS - 1 N2 - The study was conducted during a suspected epidemic of leptospirosis in Maharashtra. A total of 13 acute phase blood samples, collected at 5-6 days from the onset of symptoms, and 10 convalescent phase samples, colected at around 20 days from the onset of symptoms were obtained from 13 patients. Sera were separated and the samples were subjected to Microagglutination Test (MAT) and IgM-Indirect Immunofluorescence assay (IFA) to detect antibodies against leptospira. In the acute phase sera, only one sample was positive by MAT while seven were positive by IFA. In the convalescent samples, six were positive by MAT and seven were positive by IFA. IFA is a rapid test and can be used for early diagnosis of leptospirosis. SN - 0377-4929 UR - https://www.unboundmedicine.com/medline/citation/12593569/Role_of_IgM_specific_indirect_immunofluorescence_assay_in_diagnosing_an_outbreak_of_leptospirosis_ L2 - https://www.diseaseinfosearch.org/result/4178 DB - PRIME DP - Unbound Medicine ER -