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HilE interacts with HilD and negatively regulates hilA transcription and expression of the Salmonella enterica serovar Typhimurium invasive phenotype.
Infect Immun. 2003 Mar; 71(3):1295-305.II

Abstract

The ability of Salmonella enterica serovar Typhimurium to traverse the intestinal mucosa of a host is an important step in its ability to initiate gastrointestinal disease. The majority of the genes required for this invasive characteristic are encoded on Salmonella pathogenicity island 1 (SPI1), and their expression is controlled by the transcriptional activator HilA, a member of the OmpR/ToxR family of proteins. A variety of genes (hilC, hilD, fis, sirA/barA, csrAB, phoB, fadD, envZ/ompR, fliZ, hilE, ams, lon, pag, and hha) have been identified that exert positive or negative effects on hilA expression, although the mechanisms by which these gene products function remain relatively unclear. Recent work indicates that the small DNA-binding protein, Hha, has a significant role in repressing hilA transcription and the invasive phenotype, particularly in response to osmolarity signals. We have characterized the Salmonella-specific gene, hilE, and found that it plays an important regulatory role in hilA transcription and invasion gene expression. Mutation of hilE causes derepression of hilA transcription, and overexpression of hilE superrepresses hilA expression and the invasive phenotype. Bacterial two-hybrid experiments indicate that the HilE protein interacts with HilD, suggesting a possible mechanism for HilE negative regulation of hilA gene expression and the Salmonella invasive phenotype. Finally, we have found that the hilE gene resides on a region of the serovar Typhimurium chromosome that has many characteristics of a pathogenicity island.

Authors+Show Affiliations

Genetics Program, University of Iowa School of Medicine, Iowa City, Iowa 52242, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

12595445

Citation

Baxter, M Aaron, et al. "HilE Interacts With HilD and Negatively Regulates hilA Transcription and Expression of the Salmonella Enterica Serovar Typhimurium Invasive Phenotype." Infection and Immunity, vol. 71, no. 3, 2003, pp. 1295-305.
Baxter MA, Fahlen TF, Wilson RL, et al. HilE interacts with HilD and negatively regulates hilA transcription and expression of the Salmonella enterica serovar Typhimurium invasive phenotype. Infect Immun. 2003;71(3):1295-305.
Baxter, M. A., Fahlen, T. F., Wilson, R. L., & Jones, B. D. (2003). HilE interacts with HilD and negatively regulates hilA transcription and expression of the Salmonella enterica serovar Typhimurium invasive phenotype. Infection and Immunity, 71(3), 1295-305.
Baxter MA, et al. HilE Interacts With HilD and Negatively Regulates hilA Transcription and Expression of the Salmonella Enterica Serovar Typhimurium Invasive Phenotype. Infect Immun. 2003;71(3):1295-305. PubMed PMID: 12595445.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - HilE interacts with HilD and negatively regulates hilA transcription and expression of the Salmonella enterica serovar Typhimurium invasive phenotype. AU - Baxter,M Aaron, AU - Fahlen,Thomas F, AU - Wilson,Rebecca L, AU - Jones,Bradley D, PY - 2003/2/22/pubmed PY - 2003/3/21/medline PY - 2003/2/22/entrez SP - 1295 EP - 305 JF - Infection and immunity JO - Infect Immun VL - 71 IS - 3 N2 - The ability of Salmonella enterica serovar Typhimurium to traverse the intestinal mucosa of a host is an important step in its ability to initiate gastrointestinal disease. The majority of the genes required for this invasive characteristic are encoded on Salmonella pathogenicity island 1 (SPI1), and their expression is controlled by the transcriptional activator HilA, a member of the OmpR/ToxR family of proteins. A variety of genes (hilC, hilD, fis, sirA/barA, csrAB, phoB, fadD, envZ/ompR, fliZ, hilE, ams, lon, pag, and hha) have been identified that exert positive or negative effects on hilA expression, although the mechanisms by which these gene products function remain relatively unclear. Recent work indicates that the small DNA-binding protein, Hha, has a significant role in repressing hilA transcription and the invasive phenotype, particularly in response to osmolarity signals. We have characterized the Salmonella-specific gene, hilE, and found that it plays an important regulatory role in hilA transcription and invasion gene expression. Mutation of hilE causes derepression of hilA transcription, and overexpression of hilE superrepresses hilA expression and the invasive phenotype. Bacterial two-hybrid experiments indicate that the HilE protein interacts with HilD, suggesting a possible mechanism for HilE negative regulation of hilA gene expression and the Salmonella invasive phenotype. Finally, we have found that the hilE gene resides on a region of the serovar Typhimurium chromosome that has many characteristics of a pathogenicity island. SN - 0019-9567 UR - https://www.unboundmedicine.com/medline/citation/12595445/HilE_interacts_with_HilD_and_negatively_regulates_hilA_transcription_and_expression_of_the_Salmonella_enterica_serovar_Typhimurium_invasive_phenotype_ DB - PRIME DP - Unbound Medicine ER -