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Genetic analysis of loci associated with partial resistance to Sclerotinia sclerotiorum in rapeseed (Brassica napus L.).
Theor Appl Genet. 2003 Feb; 106(4):759-64.TA

Abstract

Sclerotinia stem rot is the most devastating disease of rapeseed (Brassica napus L.) in China. Quantitative trait loci (QTLs) involved in resistance to Sclerotinia sclerotiorum were detected in a rapeseed population of 128-F(2:3) families derived from a cross between the male sterility restorer line H5200 and a partial resistant line Ning RS-1. A total of 107 molecular markers including 72 RFLPs, 30 AFLPs, 3 SSRs and 2 RAPDs were employed to construct a genetic linkage map with 23 linkage groups covering 1,625.7 cM with an average space of 15.2 cM. Resistance was assessed empirically at two developmental stages: with a detached leaf inoculation at the seedling stage and in vivo stem inoculation at the mature plant stage. The observed resistance was scored for each plant as leaf resistance at the seedling stage (LRS) and stem resistance at the mature plant stage (SRM). A total of 13 loci were identified by one-way ANOVA and six QTLs were detected with MapMaker-QTL. We found that three of the six QTLs were associated with leaf resistance at the seedling stage and collectively accounted for 40.7% of the total phenotypic variation, each accounting for 23.2%, 16.6% and 13.6% respectively. Three QTLs were found corresponding to the disease resistance at the mature plant stage, explaining 49.0% of the phenotypic variation. Epistasis was observed for the resistance and the additive by additive interactions were the predominant type of epistasis. It was concluded that both single-locus QTLs and epistatic interactions played important roles in Sclerotinia resistance in rapeseed.

Authors+Show Affiliations

National Key Laboratory of Crop Genetic Improvement, National Center of Crop Molecular Breeding, Huazhong Agricultural University, Wuhan 430070, China.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12596007

Citation

Zhao, Jianwei, and Jinling Meng. "Genetic Analysis of Loci Associated With Partial Resistance to Sclerotinia Sclerotiorum in Rapeseed (Brassica Napus L.)." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 106, no. 4, 2003, pp. 759-64.
Zhao J, Meng J. Genetic analysis of loci associated with partial resistance to Sclerotinia sclerotiorum in rapeseed (Brassica napus L.). Theor Appl Genet. 2003;106(4):759-64.
Zhao, J., & Meng, J. (2003). Genetic analysis of loci associated with partial resistance to Sclerotinia sclerotiorum in rapeseed (Brassica napus L.). TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 106(4), 759-64.
Zhao J, Meng J. Genetic Analysis of Loci Associated With Partial Resistance to Sclerotinia Sclerotiorum in Rapeseed (Brassica Napus L.). Theor Appl Genet. 2003;106(4):759-64. PubMed PMID: 12596007.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genetic analysis of loci associated with partial resistance to Sclerotinia sclerotiorum in rapeseed (Brassica napus L.). AU - Zhao,Jianwei, AU - Meng,Jinling, Y1 - 2002/12/12/ PY - 2002/01/20/received PY - 2002/09/16/accepted PY - 2003/2/22/pubmed PY - 2003/9/30/medline PY - 2003/2/22/entrez SP - 759 EP - 64 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor. Appl. Genet. VL - 106 IS - 4 N2 - Sclerotinia stem rot is the most devastating disease of rapeseed (Brassica napus L.) in China. Quantitative trait loci (QTLs) involved in resistance to Sclerotinia sclerotiorum were detected in a rapeseed population of 128-F(2:3) families derived from a cross between the male sterility restorer line H5200 and a partial resistant line Ning RS-1. A total of 107 molecular markers including 72 RFLPs, 30 AFLPs, 3 SSRs and 2 RAPDs were employed to construct a genetic linkage map with 23 linkage groups covering 1,625.7 cM with an average space of 15.2 cM. Resistance was assessed empirically at two developmental stages: with a detached leaf inoculation at the seedling stage and in vivo stem inoculation at the mature plant stage. The observed resistance was scored for each plant as leaf resistance at the seedling stage (LRS) and stem resistance at the mature plant stage (SRM). A total of 13 loci were identified by one-way ANOVA and six QTLs were detected with MapMaker-QTL. We found that three of the six QTLs were associated with leaf resistance at the seedling stage and collectively accounted for 40.7% of the total phenotypic variation, each accounting for 23.2%, 16.6% and 13.6% respectively. Three QTLs were found corresponding to the disease resistance at the mature plant stage, explaining 49.0% of the phenotypic variation. Epistasis was observed for the resistance and the additive by additive interactions were the predominant type of epistasis. It was concluded that both single-locus QTLs and epistatic interactions played important roles in Sclerotinia resistance in rapeseed. SN - 0040-5752 UR - https://www.unboundmedicine.com/medline/citation/12596007/Genetic_analysis_of_loci_associated_with_partial_resistance_to_Sclerotinia_sclerotiorum_in_rapeseed__Brassica_napus_L___ L2 - https://dx.doi.org/10.1007/s00122-002-1171-2 DB - PRIME DP - Unbound Medicine ER -