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Dietary lipids alter the effect of steroids on the transport of glucose after intestinal resection: Part I. Phenotypic changes and expression of transporters.
J Pediatr Surg 2003; 38(2):150-60JP

Abstract

BACKGROUND/PURPOSE

Glucocorticosteroids alter the function of the intestine. This study was undertaken to assess the effect on D-glucose uptake of budesonide (Bud), prednisone (Pred), or dexamethasone (Dex) in animals with a 50% intestinal resection and fed chow or a diet enriched with saturated (SFA) or polyunsaturated fatty acids (PUFA).

METHODS

In vitro ring uptake technique, Western blots, and Northern blots were performed.

RESULTS

Bud increased the jejunal D-glucose uptake, and this effect was prevented by feeding PUFA. SGLT1 and Na+/K+ ATPase protein and mRNA abundance did not correlate with the change in the rate of uptake of glucose.

CONCLUSIONS

(1) Bud increased the jejunal glucose uptake, (2) the activity of the sugar transporter does not correlate with the abundance of protein or their respective mRNAs, (3) th Bud effect on glucose uptake is prevented by feeding PUFA. Thus, the desired intestinal adaptive response after intestinal resection may be enhanced further by the administration of the locally acting steroid budesonide and by feeding a saturated compared with a polyunsaturated fatty acid diet.

Authors+Show Affiliations

Nutrition and Metabolism Research Group, Division of Gastroenterology, Department of Medicine, University of Alberta, Edmonton, Canada.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12596094

Citation

Thiesen, Aducio L., et al. "Dietary Lipids Alter the Effect of Steroids On the Transport of Glucose After Intestinal Resection: Part I. Phenotypic Changes and Expression of Transporters." Journal of Pediatric Surgery, vol. 38, no. 2, 2003, pp. 150-60.
Thiesen AL, Tappenden KA, McBurney MI, et al. Dietary lipids alter the effect of steroids on the transport of glucose after intestinal resection: Part I. Phenotypic changes and expression of transporters. J Pediatr Surg. 2003;38(2):150-60.
Thiesen, A. L., Tappenden, K. A., McBurney, M. I., Clandinin, M. T., Keelan, M., Thomson, B. K., ... Thomson, A. B. (2003). Dietary lipids alter the effect of steroids on the transport of glucose after intestinal resection: Part I. Phenotypic changes and expression of transporters. Journal of Pediatric Surgery, 38(2), pp. 150-60.
Thiesen AL, et al. Dietary Lipids Alter the Effect of Steroids On the Transport of Glucose After Intestinal Resection: Part I. Phenotypic Changes and Expression of Transporters. J Pediatr Surg. 2003;38(2):150-60. PubMed PMID: 12596094.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dietary lipids alter the effect of steroids on the transport of glucose after intestinal resection: Part I. Phenotypic changes and expression of transporters. AU - Thiesen,Aducio L, AU - Tappenden,Kelly A, AU - McBurney,Mike I, AU - Clandinin,M Thomas, AU - Keelan,Monika, AU - Thomson,Ben K, AU - Wild,Gary E, AU - Thomson,Alan B, PY - 2003/2/22/pubmed PY - 2003/12/3/medline PY - 2003/2/22/entrez SP - 150 EP - 60 JF - Journal of pediatric surgery JO - J. Pediatr. Surg. VL - 38 IS - 2 N2 - BACKGROUND/PURPOSE: Glucocorticosteroids alter the function of the intestine. This study was undertaken to assess the effect on D-glucose uptake of budesonide (Bud), prednisone (Pred), or dexamethasone (Dex) in animals with a 50% intestinal resection and fed chow or a diet enriched with saturated (SFA) or polyunsaturated fatty acids (PUFA). METHODS: In vitro ring uptake technique, Western blots, and Northern blots were performed. RESULTS: Bud increased the jejunal D-glucose uptake, and this effect was prevented by feeding PUFA. SGLT1 and Na+/K+ ATPase protein and mRNA abundance did not correlate with the change in the rate of uptake of glucose. CONCLUSIONS: (1) Bud increased the jejunal glucose uptake, (2) the activity of the sugar transporter does not correlate with the abundance of protein or their respective mRNAs, (3) th Bud effect on glucose uptake is prevented by feeding PUFA. Thus, the desired intestinal adaptive response after intestinal resection may be enhanced further by the administration of the locally acting steroid budesonide and by feeding a saturated compared with a polyunsaturated fatty acid diet. SN - 1531-5037 UR - https://www.unboundmedicine.com/medline/citation/12596094/Dietary_lipids_alter_the_effect_of_steroids_on_the_transport_of_glucose_after_intestinal_resection:_Part_I__Phenotypic_changes_and_expression_of_transporters_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022346802630428 DB - PRIME DP - Unbound Medicine ER -