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Mitochondrial and peroxisomal manganese superoxide dismutase: differential expression during leaf senescence.
J Exp Bot. 2003 Mar; 54(384):923-33.JE

Abstract

In pea (Pisum sativum L.) leaves, manganese superoxide dismutase (Mn-SOD) is mainly localized in mitochondria as well as in peroxisomes. In this work, the effect of leaf senescence on the peroxisomal and mitochondrial Mn-SOD was studied in detached leaves from pea plants which were incubated in the dark at 25 degrees C for 3-11 d. Northern blots hybridized with a cDNA encoding mitochondrial Mn-SOD from pea leaves and a mitochondrial Mn-SOD transit peptide-specific probe showed increased Mn-SOD transcript levels during leaf senescence, due in part to in creased mitochondrial Mn-SOD mRNA. Recombinantly-expressed mitochondrial Mn-SOD was used to raise polyclonal antibodies which cross-reacted with Mn-SOD in peroxisomes purified from pea leaves. Western blot assays of crude extracts with the antibodies to pea mitochondrial Mn-SOD showed that the levels of total Mn-SOD protein gradually increased with leaf senescence. By native PAGE, the total Mn-SOD activity of pea leaves increased with senescence. EM immunocytochemistry was used to distinguish mitochondrial and peroxisomal Mn-SOD in senescent leaves. Increased Mn-SOD labelling in mitochondria was intensified, whereas the immunogold labelling of peroxisomes did not change with senescence. Overall, these results show that mitochondrial and peroxisomal Mn-SOD expression is regulated differently. The expression of mitochondrial Mn-SOD is induced during the senescence of pea leaves, whereas peroxisomal Mn-SOD could be post-translationally activated. Previously described results showing decreased mitochondrial Mn-SOD activity and increased peroxisomal Mn-SOD activity may be reflective of post-translational events regulating enzymatic activity during leaf senescence.

Authors+Show Affiliations

Department of Plant Science, Rutgers University, New Brunswick, NJ, USA. luisalfonzo.delrio@eez.csic.esNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

12598563

Citation

del Río, Luis A., et al. "Mitochondrial and Peroxisomal Manganese Superoxide Dismutase: Differential Expression During Leaf Senescence." Journal of Experimental Botany, vol. 54, no. 384, 2003, pp. 923-33.
del Río LA, Sandalio LM, Altomare DA, et al. Mitochondrial and peroxisomal manganese superoxide dismutase: differential expression during leaf senescence. J Exp Bot. 2003;54(384):923-33.
del Río, L. A., Sandalio, L. M., Altomare, D. A., & Zilinskas, B. A. (2003). Mitochondrial and peroxisomal manganese superoxide dismutase: differential expression during leaf senescence. Journal of Experimental Botany, 54(384), 923-33.
del Río LA, et al. Mitochondrial and Peroxisomal Manganese Superoxide Dismutase: Differential Expression During Leaf Senescence. J Exp Bot. 2003;54(384):923-33. PubMed PMID: 12598563.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mitochondrial and peroxisomal manganese superoxide dismutase: differential expression during leaf senescence. AU - del Río,Luis A, AU - Sandalio,Luisa M, AU - Altomare,Deborah A, AU - Zilinskas,Barbara A, PY - 2003/2/25/pubmed PY - 2003/5/16/medline PY - 2003/2/25/entrez SP - 923 EP - 33 JF - Journal of experimental botany JO - J Exp Bot VL - 54 IS - 384 N2 - In pea (Pisum sativum L.) leaves, manganese superoxide dismutase (Mn-SOD) is mainly localized in mitochondria as well as in peroxisomes. In this work, the effect of leaf senescence on the peroxisomal and mitochondrial Mn-SOD was studied in detached leaves from pea plants which were incubated in the dark at 25 degrees C for 3-11 d. Northern blots hybridized with a cDNA encoding mitochondrial Mn-SOD from pea leaves and a mitochondrial Mn-SOD transit peptide-specific probe showed increased Mn-SOD transcript levels during leaf senescence, due in part to in creased mitochondrial Mn-SOD mRNA. Recombinantly-expressed mitochondrial Mn-SOD was used to raise polyclonal antibodies which cross-reacted with Mn-SOD in peroxisomes purified from pea leaves. Western blot assays of crude extracts with the antibodies to pea mitochondrial Mn-SOD showed that the levels of total Mn-SOD protein gradually increased with leaf senescence. By native PAGE, the total Mn-SOD activity of pea leaves increased with senescence. EM immunocytochemistry was used to distinguish mitochondrial and peroxisomal Mn-SOD in senescent leaves. Increased Mn-SOD labelling in mitochondria was intensified, whereas the immunogold labelling of peroxisomes did not change with senescence. Overall, these results show that mitochondrial and peroxisomal Mn-SOD expression is regulated differently. The expression of mitochondrial Mn-SOD is induced during the senescence of pea leaves, whereas peroxisomal Mn-SOD could be post-translationally activated. Previously described results showing decreased mitochondrial Mn-SOD activity and increased peroxisomal Mn-SOD activity may be reflective of post-translational events regulating enzymatic activity during leaf senescence. SN - 0022-0957 UR - https://www.unboundmedicine.com/medline/citation/12598563/Mitochondrial_and_peroxisomal_manganese_superoxide_dismutase:_differential_expression_during_leaf_senescence_ L2 - https://academic.oup.com/jxb/article-lookup/doi/10.1093/jxb/erg091 DB - PRIME DP - Unbound Medicine ER -