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Sequencing of human-viral DNA junctions in hepatocellular carcinoma from patients with HCV and occult HBV infection.
J Med Virol 2003; 69(4):475-81JM

Abstract

DNA of free hepatitis B viruses (HBV) has been detected in the liver of patients infected with hepatitis C virus (HCV). It is unknown whether HBV DNA is integrated into such livers; if so, it may affect hepatocarcinogenesis. Hepatocellular carcinomas (HCCs) from 34 patients without HBV surface antigen (HBsAg) and with anti-HCV, and from 7 patients with HBsAg and without anti-HCV as controls, were examined, using the cassette-ligation-mediated polymerase chain reaction and primers based on HBV DNA sequence. In the controls, HBV DNA had been integrated into human DNA of all HCCs. On the basis of HBV DNA in tumor tissue, 23 of the 34 patients with anti-HCV had occult infection. Junctions between human DNA and HBV DNA were detected in 10 of the 34 patients without HBsAg and with anti-HCV. HBV DNA was integrated into chromosome 11q in 4 of the 10 HCCs with junctions. The DNA to either side of the human-viral junctions was sequenced. Clinically, the mean tumor size of these 10 HCCs was 39 mm; that of the 24 HCCs without integrated HBV was 25 mm. The surrounding tissue was cirrhotic in 2 of the 10 former HCCs and in 16 of the latter 24 HCCs. In conclusion, integrated HBV was detected in some patients with HCV infection; in these patients, the integrated DNA was associated with accelerated hepatocarcinogenesis.

Authors+Show Affiliations

Department of Hepatology, Osaka City University Graduate School of Medicine, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12601754

Citation

Tamori, Akihiro, et al. "Sequencing of Human-viral DNA Junctions in Hepatocellular Carcinoma From Patients With HCV and Occult HBV Infection." Journal of Medical Virology, vol. 69, no. 4, 2003, pp. 475-81.
Tamori A, Nishiguchi S, Kubo S, et al. Sequencing of human-viral DNA junctions in hepatocellular carcinoma from patients with HCV and occult HBV infection. J Med Virol. 2003;69(4):475-81.
Tamori, A., Nishiguchi, S., Kubo, S., Enomoto, M., Koh, N., Takeda, T., ... Otani, S. (2003). Sequencing of human-viral DNA junctions in hepatocellular carcinoma from patients with HCV and occult HBV infection. Journal of Medical Virology, 69(4), pp. 475-81.
Tamori A, et al. Sequencing of Human-viral DNA Junctions in Hepatocellular Carcinoma From Patients With HCV and Occult HBV Infection. J Med Virol. 2003;69(4):475-81. PubMed PMID: 12601754.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sequencing of human-viral DNA junctions in hepatocellular carcinoma from patients with HCV and occult HBV infection. AU - Tamori,Akihiro, AU - Nishiguchi,Shuhei, AU - Kubo,Shoji, AU - Enomoto,Masaru, AU - Koh,Noritoshi, AU - Takeda,Tadashi, AU - Shiomi,Susumu, AU - Hirohashi,Kazuhiro, AU - Kinoshita,Hiroaki, AU - Otani,Shuzo, PY - 2003/2/26/pubmed PY - 2003/5/2/medline PY - 2003/2/26/entrez SP - 475 EP - 81 JF - Journal of medical virology JO - J. Med. Virol. VL - 69 IS - 4 N2 - DNA of free hepatitis B viruses (HBV) has been detected in the liver of patients infected with hepatitis C virus (HCV). It is unknown whether HBV DNA is integrated into such livers; if so, it may affect hepatocarcinogenesis. Hepatocellular carcinomas (HCCs) from 34 patients without HBV surface antigen (HBsAg) and with anti-HCV, and from 7 patients with HBsAg and without anti-HCV as controls, were examined, using the cassette-ligation-mediated polymerase chain reaction and primers based on HBV DNA sequence. In the controls, HBV DNA had been integrated into human DNA of all HCCs. On the basis of HBV DNA in tumor tissue, 23 of the 34 patients with anti-HCV had occult infection. Junctions between human DNA and HBV DNA were detected in 10 of the 34 patients without HBsAg and with anti-HCV. HBV DNA was integrated into chromosome 11q in 4 of the 10 HCCs with junctions. The DNA to either side of the human-viral junctions was sequenced. Clinically, the mean tumor size of these 10 HCCs was 39 mm; that of the 24 HCCs without integrated HBV was 25 mm. The surrounding tissue was cirrhotic in 2 of the 10 former HCCs and in 16 of the latter 24 HCCs. In conclusion, integrated HBV was detected in some patients with HCV infection; in these patients, the integrated DNA was associated with accelerated hepatocarcinogenesis. SN - 0146-6615 UR - https://www.unboundmedicine.com/medline/citation/12601754/Sequencing_of_human_viral_DNA_junctions_in_hepatocellular_carcinoma_from_patients_with_HCV_and_occult_HBV_infection_ L2 - https://doi.org/10.1002/jmv.10334 DB - PRIME DP - Unbound Medicine ER -