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Genotoxicity of a polluted river system measured using the alkaline comet assay on fish and earthworm tissues.
Environ Mol Mutagen. 2003; 41(2):85-91.EM

Abstract

Monitoring genotoxicity in the environment by using endemic organisms as sentinels requires sensitive assays. In this study the genotoxic properties of water and sediment collected from the Noyyal River, which is polluted with industrial effluent and sewage, was determined in fish (Cyprinus carpio) and earthworms (Eisenia foetida) using the alkaline comet assay. Upon electrophoresis, extensive DNA damage, measured as the DNA length:width ratio of the DNA mass, was observed in erythrocytes, liver, and kidney cells of fish exposed to polluted water samples and the amount of damage increased with the duration of exposure. Similarly, the mean DNA length:width ratio was significantly higher in the coelomocytes of earthworms placed in sediment samples. The highest levels of DNA damage were obtained with samples taken at and immediately downstream of urban centers. The results of this study indicate that the Noyyal River system is contaminated with substances that are genotoxic to fish and earthworms and that the comet assay has sufficient sensitivity to detect the genotoxicity.

Authors+Show Affiliations

Department of Environmental Science, PSG College of Arts & Science, Coimbatore, India.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12605376

Citation

Rajaguru, P, et al. "Genotoxicity of a Polluted River System Measured Using the Alkaline Comet Assay On Fish and Earthworm Tissues." Environmental and Molecular Mutagenesis, vol. 41, no. 2, 2003, pp. 85-91.
Rajaguru P, Suba S, Palanivel M, et al. Genotoxicity of a polluted river system measured using the alkaline comet assay on fish and earthworm tissues. Environ Mol Mutagen. 2003;41(2):85-91.
Rajaguru, P., Suba, S., Palanivel, M., & Kalaiselvi, K. (2003). Genotoxicity of a polluted river system measured using the alkaline comet assay on fish and earthworm tissues. Environmental and Molecular Mutagenesis, 41(2), 85-91.
Rajaguru P, et al. Genotoxicity of a Polluted River System Measured Using the Alkaline Comet Assay On Fish and Earthworm Tissues. Environ Mol Mutagen. 2003;41(2):85-91. PubMed PMID: 12605376.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genotoxicity of a polluted river system measured using the alkaline comet assay on fish and earthworm tissues. AU - Rajaguru,P, AU - Suba,S, AU - Palanivel,M, AU - Kalaiselvi,K, PY - 2003/2/28/pubmed PY - 2003/4/24/medline PY - 2003/2/28/entrez SP - 85 EP - 91 JF - Environmental and molecular mutagenesis JO - Environ Mol Mutagen VL - 41 IS - 2 N2 - Monitoring genotoxicity in the environment by using endemic organisms as sentinels requires sensitive assays. In this study the genotoxic properties of water and sediment collected from the Noyyal River, which is polluted with industrial effluent and sewage, was determined in fish (Cyprinus carpio) and earthworms (Eisenia foetida) using the alkaline comet assay. Upon electrophoresis, extensive DNA damage, measured as the DNA length:width ratio of the DNA mass, was observed in erythrocytes, liver, and kidney cells of fish exposed to polluted water samples and the amount of damage increased with the duration of exposure. Similarly, the mean DNA length:width ratio was significantly higher in the coelomocytes of earthworms placed in sediment samples. The highest levels of DNA damage were obtained with samples taken at and immediately downstream of urban centers. The results of this study indicate that the Noyyal River system is contaminated with substances that are genotoxic to fish and earthworms and that the comet assay has sufficient sensitivity to detect the genotoxicity. SN - 0893-6692 UR - https://www.unboundmedicine.com/medline/citation/12605376/Genotoxicity_of_a_polluted_river_system_measured_using_the_alkaline_comet_assay_on_fish_and_earthworm_tissues_ L2 - https://doi.org/10.1002/em.10134 DB - PRIME DP - Unbound Medicine ER -