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Detecting K-ras and p53 gene mutation from stool and pancreatic juice for diagnosis of early pancreatic cancer.
Chin Med J (Engl) 2002; 115(11):1632-6CM

Abstract

OBJECTIVE

To explore new methods for the early diagnosis of pancreatic cancer through detection of K-ras and p53 mutations in pancreatic juice and stool.

METHODS

201 patients in PUMC Hospital from 1994 - 2000 and 60 control individuals were enrolled in this study. K-ras point mutation was detected by PCR-RFLP while p53 mutation was detected by PCR-SSCP.

RESULTS

K-ras mutation was found in pancreatic juice in 87.8% (36/41) of pancreatic cancer patients and 23.5% (4/17) of benign pancreatic disease patients. In 261 stool specimens, amplification found mutations successfully in 235 patients (90%). K-ras mutation was found in stool in 88% (66/75) of pancreatic cancer patients, 51.1% (24/47) of benign pancreatic disease patients and 19.6% (9/46) of normal individuals. p53 mutation was found in pancreatic juice in 47.4% (18/38) of pancreatic cancer patients and 12.5% (2/16) of benign pancreatic disease patients. p53 mutation was found in stool in 37.1% (23/62) and 19.1% (4/21) of chronic pancreatitis patients.

CONCLUSION

K-ras mutation in pancreatic juice has higher diagnosis sensitivity and specificity, and therefore may be used as a supplement in the diagnosis of pancreatic cancer. Detection of K-ras mutation combined with p53 mutation in stool can aid in the screening of pancreatic cancer.

Authors+Show Affiliations

Peking Union Medical Hospital, Beijing 100730, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12609076

Citation

Lu, Xinghua, et al. "Detecting K-ras and P53 Gene Mutation From Stool and Pancreatic Juice for Diagnosis of Early Pancreatic Cancer." Chinese Medical Journal, vol. 115, no. 11, 2002, pp. 1632-6.
Lu X, Xu T, Qian J, et al. Detecting K-ras and p53 gene mutation from stool and pancreatic juice for diagnosis of early pancreatic cancer. Chin Med J. 2002;115(11):1632-6.
Lu, X., Xu, T., Qian, J., Wen, X., & Wu, D. (2002). Detecting K-ras and p53 gene mutation from stool and pancreatic juice for diagnosis of early pancreatic cancer. Chinese Medical Journal, 115(11), pp. 1632-6.
Lu X, et al. Detecting K-ras and P53 Gene Mutation From Stool and Pancreatic Juice for Diagnosis of Early Pancreatic Cancer. Chin Med J. 2002;115(11):1632-6. PubMed PMID: 12609076.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detecting K-ras and p53 gene mutation from stool and pancreatic juice for diagnosis of early pancreatic cancer. AU - Lu,Xinghua, AU - Xu,Tong, AU - Qian,Jiaming, AU - Wen,Xiaoheng, AU - Wu,Dongsheng, PY - 2003/3/1/pubmed PY - 2003/5/6/medline PY - 2003/3/1/entrez SP - 1632 EP - 6 JF - Chinese medical journal JO - Chin. Med. J. VL - 115 IS - 11 N2 - OBJECTIVE: To explore new methods for the early diagnosis of pancreatic cancer through detection of K-ras and p53 mutations in pancreatic juice and stool. METHODS: 201 patients in PUMC Hospital from 1994 - 2000 and 60 control individuals were enrolled in this study. K-ras point mutation was detected by PCR-RFLP while p53 mutation was detected by PCR-SSCP. RESULTS: K-ras mutation was found in pancreatic juice in 87.8% (36/41) of pancreatic cancer patients and 23.5% (4/17) of benign pancreatic disease patients. In 261 stool specimens, amplification found mutations successfully in 235 patients (90%). K-ras mutation was found in stool in 88% (66/75) of pancreatic cancer patients, 51.1% (24/47) of benign pancreatic disease patients and 19.6% (9/46) of normal individuals. p53 mutation was found in pancreatic juice in 47.4% (18/38) of pancreatic cancer patients and 12.5% (2/16) of benign pancreatic disease patients. p53 mutation was found in stool in 37.1% (23/62) and 19.1% (4/21) of chronic pancreatitis patients. CONCLUSION: K-ras mutation in pancreatic juice has higher diagnosis sensitivity and specificity, and therefore may be used as a supplement in the diagnosis of pancreatic cancer. Detection of K-ras mutation combined with p53 mutation in stool can aid in the screening of pancreatic cancer. SN - 0366-6999 UR - https://www.unboundmedicine.com/medline/citation/12609076/Detecting_K_ras_and_p53_gene_mutation_from_stool_and_pancreatic_juice_for_diagnosis_of_early_pancreatic_cancer_ L2 - http://www.diseaseinfosearch.org/result/5551 DB - PRIME DP - Unbound Medicine ER -