Tags

Type your tag names separated by a space and hit enter

Detection of rhinovirus and enterovirus in upper respiratory tract samples using a multiplex nested PCR.
J Virol Methods. 2003 Mar; 108(2):223-8.JV

Abstract

Human enteroviruses and rhinoviruses are respiratory pathogens whose role in acute respiratory infection is underestimated due to the use of diagnostic procedures with poor sensitivity. To determine the prevalence of these two pathogens in the upper respiratory tract infections, a multiplex procedure was developed that both detect and differentiate rhinoviruses and enteroviruses. This sensitive procedure allowed the detection of both pathogens from archival material (nasal swabs) collected during the previous winters and differentiated rhinoviruses from enteroviruses. This procedure can be used to determine the role of these pathogens in mild or severe upper respiratory tract infections.

Authors+Show Affiliations

Billaud G
CNR Enterovirus et CNR Grippe (France sud), Laboratoire de Virologie des Hospices Civils de Lyon, UMR 5537, Domaine Rockefeller, 69373 Lyon Cedex 08, France.
Peny S
No affiliation info available
Legay V
No affiliation info available
Lina B
No affiliation info available
Valette M
No affiliation info available

MeSH

AnimalsBase SequenceCell LineDNA PrimersDogsEnterovirusEnterovirus InfectionsHumansPicornaviridae InfectionsPolymerase Chain ReactionRespiratory Tract InfectionsRhinovirusSensitivity and SpecificityVirology

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

12609690

Citation

Billaud, Geneviève, et al. "Detection of Rhinovirus and Enterovirus in Upper Respiratory Tract Samples Using a Multiplex Nested PCR." Journal of Virological Methods, vol. 108, no. 2, 2003, pp. 223-8.
Billaud G, Peny S, Legay V, et al. Detection of rhinovirus and enterovirus in upper respiratory tract samples using a multiplex nested PCR. J Virol Methods. 2003;108(2):223-8.
Billaud, G., Peny, S., Legay, V., Lina, B., & Valette, M. (2003). Detection of rhinovirus and enterovirus in upper respiratory tract samples using a multiplex nested PCR. Journal of Virological Methods, 108(2), 223-8.
Billaud G, et al. Detection of Rhinovirus and Enterovirus in Upper Respiratory Tract Samples Using a Multiplex Nested PCR. J Virol Methods. 2003;108(2):223-8. PubMed PMID: 12609690.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of rhinovirus and enterovirus in upper respiratory tract samples using a multiplex nested PCR. AU - Billaud,Geneviève, AU - Peny,Stéphanie, AU - Legay,Vincent, AU - Lina,Bruno, AU - Valette,Martine, PY - 2003/3/1/pubmed PY - 2003/5/28/medline PY - 2003/3/1/entrez SP - 223 EP - 8 JF - Journal of virological methods JO - J Virol Methods VL - 108 IS - 2 N2 - Human enteroviruses and rhinoviruses are respiratory pathogens whose role in acute respiratory infection is underestimated due to the use of diagnostic procedures with poor sensitivity. To determine the prevalence of these two pathogens in the upper respiratory tract infections, a multiplex procedure was developed that both detect and differentiate rhinoviruses and enteroviruses. This sensitive procedure allowed the detection of both pathogens from archival material (nasal swabs) collected during the previous winters and differentiated rhinoviruses from enteroviruses. This procedure can be used to determine the role of these pathogens in mild or severe upper respiratory tract infections. SN - 0166-0934 UR - https://www.unboundmedicine.com/medline/citation/12609690/Detection_of_rhinovirus_and_enterovirus_in_upper_respiratory_tract_samples_using_a_multiplex_nested_PCR_ DB - PRIME DP - Unbound Medicine ER -