Tags

Type your tag names separated by a space and hit enter

Tissue inhibitors of matrix metalloproteinases are elevated in cerebrospinal fluid of neurodegenerative diseases.
J Neurol Sci. 2003 Mar 15; 207(1-2):71-6.JN

Abstract

Matrix metalloproteinases (MMPs) are implicated in the pathogenesis of diseases such as Alzheimer's Disease (AD) and amyotrophic lateral sclerosis (ALS). Increased expression of MMP-9 and TIMPs has been reported in postmortem AD and ALS brain tissue, as well as in ALS cerebrospinal fluid (CSF) and plasma. Although individual studies of MMP and TIMP expression in CSF have included AD and ALS samples, there are no studies comparing the expression of these proteins between neurodegenerative diseases. We measured the levels of matrix metalloproteinases (MMPs)-2 and -9 and the tissue inhibitor of MMPs (e.g. TIMP-1 and TIMP-2) in CSF samples from patients with Parkinson's Disease (PD), Huntington's Disease (HD), AD and ALS as compared to age-matched control patients. There was constitutive expression of the proform of gelatinase A (proMMP-2) on zymography gels in all CSF samples. Unexpectedly, there was an additional gelatinolytic band at 130 kDa of unknown etiology in the CSF samples of patients with PD (61% of patients studied), AD (61%), HD (25%) and ALS (39%). Levels of TIMP-1 were significantly elevated in CSF samples from all disease groups. TIMP-2 was significantly increased in CSF of AD and HD patients. MMP-2 levels did not differ significantly between groups. These findings show that TIMPs are elevated in the CSF of patients with neurodegenerative diseases suggesting a potential role of these endogenous inhibitors of matrix metalloproteinases in neurodegenerative diseases.

Authors+Show Affiliations

Department of Neurology and Neuroscience, Weill Medical College of Cornell University, 525 East 68th Street Room A-501, New York, NY 10021, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

12614934

Citation

Lorenzl, S, et al. "Tissue Inhibitors of Matrix Metalloproteinases Are Elevated in Cerebrospinal Fluid of Neurodegenerative Diseases." Journal of the Neurological Sciences, vol. 207, no. 1-2, 2003, pp. 71-6.
Lorenzl S, Albers DS, LeWitt PA, et al. Tissue inhibitors of matrix metalloproteinases are elevated in cerebrospinal fluid of neurodegenerative diseases. J Neurol Sci. 2003;207(1-2):71-6.
Lorenzl, S., Albers, D. S., LeWitt, P. A., Chirichigno, J. W., Hilgenberg, S. L., Cudkowicz, M. E., & Beal, M. F. (2003). Tissue inhibitors of matrix metalloproteinases are elevated in cerebrospinal fluid of neurodegenerative diseases. Journal of the Neurological Sciences, 207(1-2), 71-6.
Lorenzl S, et al. Tissue Inhibitors of Matrix Metalloproteinases Are Elevated in Cerebrospinal Fluid of Neurodegenerative Diseases. J Neurol Sci. 2003 Mar 15;207(1-2):71-6. PubMed PMID: 12614934.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Tissue inhibitors of matrix metalloproteinases are elevated in cerebrospinal fluid of neurodegenerative diseases. AU - Lorenzl,S, AU - Albers,D S, AU - LeWitt,P A, AU - Chirichigno,J W, AU - Hilgenberg,S L, AU - Cudkowicz,M E, AU - Beal,M F, PY - 2003/3/5/pubmed PY - 2003/4/24/medline PY - 2003/3/5/entrez SP - 71 EP - 6 JF - Journal of the neurological sciences JO - J Neurol Sci VL - 207 IS - 1-2 N2 - Matrix metalloproteinases (MMPs) are implicated in the pathogenesis of diseases such as Alzheimer's Disease (AD) and amyotrophic lateral sclerosis (ALS). Increased expression of MMP-9 and TIMPs has been reported in postmortem AD and ALS brain tissue, as well as in ALS cerebrospinal fluid (CSF) and plasma. Although individual studies of MMP and TIMP expression in CSF have included AD and ALS samples, there are no studies comparing the expression of these proteins between neurodegenerative diseases. We measured the levels of matrix metalloproteinases (MMPs)-2 and -9 and the tissue inhibitor of MMPs (e.g. TIMP-1 and TIMP-2) in CSF samples from patients with Parkinson's Disease (PD), Huntington's Disease (HD), AD and ALS as compared to age-matched control patients. There was constitutive expression of the proform of gelatinase A (proMMP-2) on zymography gels in all CSF samples. Unexpectedly, there was an additional gelatinolytic band at 130 kDa of unknown etiology in the CSF samples of patients with PD (61% of patients studied), AD (61%), HD (25%) and ALS (39%). Levels of TIMP-1 were significantly elevated in CSF samples from all disease groups. TIMP-2 was significantly increased in CSF of AD and HD patients. MMP-2 levels did not differ significantly between groups. These findings show that TIMPs are elevated in the CSF of patients with neurodegenerative diseases suggesting a potential role of these endogenous inhibitors of matrix metalloproteinases in neurodegenerative diseases. SN - 0022-510X UR - https://www.unboundmedicine.com/medline/citation/12614934/Tissue_inhibitors_of_matrix_metalloproteinases_are_elevated_in_cerebrospinal_fluid_of_neurodegenerative_diseases_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022510X02003982 DB - PRIME DP - Unbound Medicine ER -