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Migration of enhanced green fluorescent protein expressing bone marrow-derived microglia/macrophage into the mouse brain following permanent focal ischemia.

Abstract

Brain ischemia induces a marked response of resident microglia and hematopoietic cells including monocytes/macrophages. The present study was designed to assess the distribution of microglia/macrophages in cerebral ischemia using bone marrow chimera mice known to express enhanced green fluorescent protein (EGFP). At 24 h after middle cerebral artery occlusion (MCAO), many round-shaped EGFP-positive cells migrated to the ischemic core and peri-infarct area. At 48-72 h after MCAO, irregular round- or oval-shaped EGFP/ionized calcium-binding adapter molecule 1 (Iba 1)-positive cells increased in the transition zone, while many amoeboid-shaped or large-cell-body EGFP/Iba 1-positive cells were increased in number in the innermost area of ischemia. At 7 days after MCAO, many process-bearing ramified shaped EGFP/Iba 1-positive cells were detected in the transition to the peri-infarct area, while phagocytic cells were distributed in the transition to the core area of the infarction. The distribution of these morphologically variable EGFP/Iba 1-positive cells was similar up to 14 days from MCAO. The present study directly showed the migration and distribution of bone marrow-derived monocytes/macrophages and the relationship between resident microglia and infiltrated hematogenous element in ischemic mouse brain. It is important to study the distribution of intrinsic and extrinsic microglia/macrophage in ischemic brain, since such findings may allow the design of appropriate gene-delivery system using exogenous microglia/macrophages to the ischemic brain area.

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  • Authors+Show Affiliations

    ,

    Department of Neurology, Juntendo University School of Medicine, 2-1-1, Hongo, Bunkyo-ku, Tokyo 113-8421, Japan.

    , , , , , , ,

    Source

    Neuroscience 117:3 2003 pg 531-9

    MeSH

    Animals
    Bone Marrow
    Brain Ischemia
    Calcium-Binding Proteins
    Cell Count
    Cell Movement
    Central Nervous System
    Chimera
    Dose-Response Relationship, Radiation
    Fluorouracil
    Green Fluorescent Proteins
    Immunohistochemistry
    Immunosuppressive Agents
    Infarction, Middle Cerebral Artery
    Luminescent Proteins
    Macrophages
    Mice
    Mice, Transgenic
    Microfilament Proteins
    Microglia
    Microtubule-Associated Proteins
    Time Factors
    Transplants
    Whole-Body Irradiation

    Pub Type(s)

    Comparative Study
    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    12617960

    Citation

    Tanaka, R, et al. "Migration of Enhanced Green Fluorescent Protein Expressing Bone Marrow-derived Microglia/macrophage Into the Mouse Brain Following Permanent Focal Ischemia." Neuroscience, vol. 117, no. 3, 2003, pp. 531-9.
    Tanaka R, Komine-Kobayashi M, Mochizuki H, et al. Migration of enhanced green fluorescent protein expressing bone marrow-derived microglia/macrophage into the mouse brain following permanent focal ischemia. Neuroscience. 2003;117(3):531-9.
    Tanaka, R., Komine-Kobayashi, M., Mochizuki, H., Yamada, M., Furuya, T., Migita, M., ... Urabe, T. (2003). Migration of enhanced green fluorescent protein expressing bone marrow-derived microglia/macrophage into the mouse brain following permanent focal ischemia. Neuroscience, 117(3), pp. 531-9.
    Tanaka R, et al. Migration of Enhanced Green Fluorescent Protein Expressing Bone Marrow-derived Microglia/macrophage Into the Mouse Brain Following Permanent Focal Ischemia. Neuroscience. 2003;117(3):531-9. PubMed PMID: 12617960.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Migration of enhanced green fluorescent protein expressing bone marrow-derived microglia/macrophage into the mouse brain following permanent focal ischemia. AU - Tanaka,R, AU - Komine-Kobayashi,M, AU - Mochizuki,H, AU - Yamada,M, AU - Furuya,T, AU - Migita,M, AU - Shimada,T, AU - Mizuno,Y, AU - Urabe,T, PY - 2003/3/6/pubmed PY - 2003/5/6/medline PY - 2003/3/6/entrez SP - 531 EP - 9 JF - Neuroscience JO - Neuroscience VL - 117 IS - 3 N2 - Brain ischemia induces a marked response of resident microglia and hematopoietic cells including monocytes/macrophages. The present study was designed to assess the distribution of microglia/macrophages in cerebral ischemia using bone marrow chimera mice known to express enhanced green fluorescent protein (EGFP). At 24 h after middle cerebral artery occlusion (MCAO), many round-shaped EGFP-positive cells migrated to the ischemic core and peri-infarct area. At 48-72 h after MCAO, irregular round- or oval-shaped EGFP/ionized calcium-binding adapter molecule 1 (Iba 1)-positive cells increased in the transition zone, while many amoeboid-shaped or large-cell-body EGFP/Iba 1-positive cells were increased in number in the innermost area of ischemia. At 7 days after MCAO, many process-bearing ramified shaped EGFP/Iba 1-positive cells were detected in the transition to the peri-infarct area, while phagocytic cells were distributed in the transition to the core area of the infarction. The distribution of these morphologically variable EGFP/Iba 1-positive cells was similar up to 14 days from MCAO. The present study directly showed the migration and distribution of bone marrow-derived monocytes/macrophages and the relationship between resident microglia and infiltrated hematogenous element in ischemic mouse brain. It is important to study the distribution of intrinsic and extrinsic microglia/macrophage in ischemic brain, since such findings may allow the design of appropriate gene-delivery system using exogenous microglia/macrophages to the ischemic brain area. SN - 0306-4522 UR - https://www.unboundmedicine.com/medline/citation/12617960/Migration_of_enhanced_green_fluorescent_protein_expressing_bone_marrow_derived_microglia/macrophage_into_the_mouse_brain_following_permanent_focal_ischemia_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0306452202009545 DB - PRIME DP - Unbound Medicine ER -