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Human telomerase reverse transcriptase (hTERT) gene expression in FNA samples from thyroid neoplasms.
Cancer Lett. 2003 Mar 10; 191(2):223-7.CL

Abstract

Identifying a tumor marker that can help distinguish benign from malignant thyroid tumors is crucial, because up to 30% of thyroid fine-needle aspirations (FNA) are diagnosed as 'suspicious' or follicular neoplasm for malignancy. Recently, the detection of human telomerase reverse transcriptase (hTERT) gene expression in thyroid FNA samples has been identified as a promising diagnostic marker in distinguishing benign and malignant thyroid tumors. Twenty-seven FNA samples from thyroid tumors that were suspected to be malignant were collected preoperatively, hTERT gene expression was examined by reverse transcriptase-polymerase chain reaction (RT-PCR), and the cytological and histological results were compared. The results demonstrated that 13 (92.8%) of 14 thyroid carcinomas, including eight of eight papillary, three of four follicular, and two of two Hürthle cell thyroid carcinomas have corresponding FNA samples that were positive for hTERT. Meanwhile, eight (61.5%) of 13 benign thyroid nodules, including three of six nodular goiter, two of two Graves' disease, two of two Hürthle cell adenomas, and one of three follicular adenomas were positive for hTERT. In conclusion, hTERT was more prevalent in malignant thyroid FNA samples than in the benign thyroid FNA samples. Notably, the extent of the differences in hTERT expression between benign and malignant follicular thyroid tumors require further investigation. Moreover, further information including semi-quantitative real-time RT-PCR, is required to verify whether hTERT mRNA expression could serve as an adjunctive molecular marker for the preoperative diagnosis of thyroid malignancies.

Authors+Show Affiliations

Section of Endocrinology and Metabolism, Department of Internal Medicine, Chang Gung Memorial Hospital and Chang Gung University, Linkou, Taiwan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

12618337

Citation

Liou, Miaw-Jene, et al. "Human Telomerase Reverse Transcriptase (hTERT) Gene Expression in FNA Samples From Thyroid Neoplasms." Cancer Letters, vol. 191, no. 2, 2003, pp. 223-7.
Liou MJ, Chan EC, Lin JD, et al. Human telomerase reverse transcriptase (hTERT) gene expression in FNA samples from thyroid neoplasms. Cancer Lett. 2003;191(2):223-7.
Liou, M. J., Chan, E. C., Lin, J. D., Liu, F. H., & Chao, T. C. (2003). Human telomerase reverse transcriptase (hTERT) gene expression in FNA samples from thyroid neoplasms. Cancer Letters, 191(2), 223-7.
Liou MJ, et al. Human Telomerase Reverse Transcriptase (hTERT) Gene Expression in FNA Samples From Thyroid Neoplasms. Cancer Lett. 2003 Mar 10;191(2):223-7. PubMed PMID: 12618337.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Human telomerase reverse transcriptase (hTERT) gene expression in FNA samples from thyroid neoplasms. AU - Liou,Miaw-Jene, AU - Chan,Err-Cheng, AU - Lin,Jen-Der, AU - Liu,Feng-Hsuan, AU - Chao,Tzu-Chieh, PY - 2003/3/6/pubmed PY - 2003/5/2/medline PY - 2003/3/6/entrez SP - 223 EP - 7 JF - Cancer letters JO - Cancer Lett VL - 191 IS - 2 N2 - Identifying a tumor marker that can help distinguish benign from malignant thyroid tumors is crucial, because up to 30% of thyroid fine-needle aspirations (FNA) are diagnosed as 'suspicious' or follicular neoplasm for malignancy. Recently, the detection of human telomerase reverse transcriptase (hTERT) gene expression in thyroid FNA samples has been identified as a promising diagnostic marker in distinguishing benign and malignant thyroid tumors. Twenty-seven FNA samples from thyroid tumors that were suspected to be malignant were collected preoperatively, hTERT gene expression was examined by reverse transcriptase-polymerase chain reaction (RT-PCR), and the cytological and histological results were compared. The results demonstrated that 13 (92.8%) of 14 thyroid carcinomas, including eight of eight papillary, three of four follicular, and two of two Hürthle cell thyroid carcinomas have corresponding FNA samples that were positive for hTERT. Meanwhile, eight (61.5%) of 13 benign thyroid nodules, including three of six nodular goiter, two of two Graves' disease, two of two Hürthle cell adenomas, and one of three follicular adenomas were positive for hTERT. In conclusion, hTERT was more prevalent in malignant thyroid FNA samples than in the benign thyroid FNA samples. Notably, the extent of the differences in hTERT expression between benign and malignant follicular thyroid tumors require further investigation. Moreover, further information including semi-quantitative real-time RT-PCR, is required to verify whether hTERT mRNA expression could serve as an adjunctive molecular marker for the preoperative diagnosis of thyroid malignancies. SN - 0304-3835 UR - https://www.unboundmedicine.com/medline/citation/12618337/Human_telomerase_reverse_transcriptase__hTERT__gene_expression_in_FNA_samples_from_thyroid_neoplasms_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S030438350200678X DB - PRIME DP - Unbound Medicine ER -