Improved detection of higher molecular weight proteins by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry on polytetrafluoroethylene surfaces.
Rapid Commun Mass Spectrom. 2003; 17(6):598-602.RC

Abstract

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) of proteins was performed on a range of polytetrafluoroethylene (PTFE) surfaces. Sinapinic acid and alpha-cyano-4-hydroxycinnamic acid matrices were compared and the order of application varied to identify the best combination for each surface. It is demonstrated that the use of a PTFE surface improves the intensity of signals obtained for higher molecular weight proteins.

Authors+Show Affiliations

Botting CH

Centre for Biomolecular Sciences, School of Chemistry, University of St Andrews, Fife KY16 9ST, Scotland, UK. cb2@st-andrews.ac.uk

MeSH

AnimalsCattleConalbuminCoumaric AcidsMolecular WeightPhosphorylase bPolytetrafluoroethyleneProteinsReproducibility of ResultsSensitivity and SpecificitySerum Albumin, BovineSpectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Pub Type(s)

Journal Article

Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12621623

Citation

Botting, Catherine H.. "Improved Detection of Higher Molecular Weight Proteins By Matrix-assisted Laser Desorption/ionization Time-of-flight Mass Spectrometry On Polytetrafluoroethylene Surfaces." Rapid Communications in Mass Spectrometry : RCM, vol. 17, no. 6, 2003, pp. 598-602.

Botting CH. Improved detection of higher molecular weight proteins by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry on polytetrafluoroethylene surfaces. Rapid Commun Mass Spectrom. 2003;17(6):598-602.

Botting, C. H. (2003). Improved detection of higher molecular weight proteins by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry on polytetrafluoroethylene surfaces. Rapid Communications in Mass Spectrometry : RCM, 17(6), 598-602.

Botting CH. Improved Detection of Higher Molecular Weight Proteins By Matrix-assisted Laser Desorption/ionization Time-of-flight Mass Spectrometry On Polytetrafluoroethylene Surfaces. Rapid Commun Mass Spectrom. 2003;17(6):598-602. PubMed PMID: 12621623.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Improved detection of higher molecular weight proteins by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry on polytetrafluoroethylene surfaces. A1 - Botting,Catherine H, PY - 2003/3/7/pubmed PY - 2003/5/7/medline PY - 2003/3/7/entrez SP - 598 EP - 602 JF - Rapid communications in mass spectrometry : RCM JO - Rapid Commun Mass Spectrom VL - 17 IS - 6 N2 - Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) of proteins was performed on a range of polytetrafluoroethylene (PTFE) surfaces. Sinapinic acid and alpha-cyano-4-hydroxycinnamic acid matrices were compared and the order of application varied to identify the best combination for each surface. It is demonstrated that the use of a PTFE surface improves the intensity of signals obtained for higher molecular weight proteins. SN - 0951-4198 UR - https://www.unboundmedicine.com/medline/citation/12621623/Improved_detection_of_higher_molecular_weight_proteins_by_matrix_assisted_laser_desorption/ionization_time_of_flight_mass_spectrometry_on_polytetrafluoroethylene_surfaces_ DB - PRIME DP - Unbound Medicine ER -

Tags

Improved detection of higher molecular weight proteins by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry on polytetrafluoroethylene surfaces.Rapid Commun Mass Spectrom. 2003; 17(6):598-602.RC