Tags

Type your tag names separated by a space and hit enter

Roasted hazelnuts--allergenic activity evaluated by double-blind, placebo-controlled food challenge.

Abstract

BACKGROUND

Allergy to hazelnuts is a common example of birch pollen related food allergy. Symptoms upon ingestion are often confined to the mouth and throat, but severe systemic reactions have been described in some patients. The aim of the study was to evaluate the reduction in allergenicity by roasting of the nuts.

METHODS

Double-blind, placebo-controlled food challenges (DBPCFC) with roasted hazelnuts (140 degrees C, 40 min) were performed in 17 birch pollen allergic patients with DBPCFC-confirmed food allergy to raw hazelnuts. The effect of roasting was further evaluated by skin prick test (SPT), histamine release (HR), measurement of specific IgE, and IgE-inhibition experiments.

RESULTS

In 5/17 patients the DBPCFC with the roasted nuts were positive. The symptoms were generally mild and included OAS (oral allergy syndrome) in all patients. Roasting of the nuts significantly reduced the allergenic activity evaluated by SPT, HR, specific IgE, and IgE-inhibition. Immunoblotting experiments with recombinant hazelnut allergens showed sensitization against Cor a 1.04 in 16/17 patients and against Cor a 2 in 7/17 patients. None of the patients were sensitized to Cor a 8. Challenge-positive patients did not differ from the rest in IgE-binding pattern.

CONCLUSIONS

All the applied methods indicated that roasting of hazelnuts reduces the allergenicity, but since 5/17 birch pollen allergic patients were DBPCFC-positive to the roasted nuts, ingestion of roasted hazelnuts or products containing roasted hazelnuts can not be considered safe for a number of hazelnut allergic consumers. For patients with a history of severe allergic symptoms upon ingestion of hazelnuts, thorough and conscientious food labelling of hazelnuts and hazelnut residues is essential.

Links

  • FREE Publisher Full Text
  • Authors+Show Affiliations

    ,

    Allergy Clinic, Department 7551, National University Hospital, Blegdamsvej 9, DK-2100 Copenhagen, Denmark.

    , , , , , ,

    Source

    Allergy 58:2 2003 Feb pg 132-8

    MeSH

    Adolescent
    Adult
    Aged
    Allergens
    Antibody Specificity
    Controlled Clinical Trials as Topic
    Cooking
    Corylus
    Denmark
    Dose-Response Relationship, Immunologic
    Double-Blind Method
    Female
    Histamine Release
    Humans
    Immunoblotting
    Immunoglobulin E
    Male
    Middle Aged
    Nut Hypersensitivity
    Pollen
    Severity of Illness Index
    Skin Tests
    Switzerland
    Syndrome

    Pub Type(s)

    Clinical Trial
    Comparative Study
    Journal Article
    Multicenter Study
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    12622744

    Citation

    Hansen, K Skamstrup, et al. "Roasted Hazelnuts--allergenic Activity Evaluated By Double-blind, Placebo-controlled Food Challenge." Allergy, vol. 58, no. 2, 2003, pp. 132-8.
    Hansen KS, Ballmer-Weber BK, Lüttkopf D, et al. Roasted hazelnuts--allergenic activity evaluated by double-blind, placebo-controlled food challenge. Allergy. 2003;58(2):132-8.
    Hansen, K. S., Ballmer-Weber, B. K., Lüttkopf, D., Skov, P. S., Wüthrich, B., Bindslev-Jensen, C., ... Poulsen, L. K. (2003). Roasted hazelnuts--allergenic activity evaluated by double-blind, placebo-controlled food challenge. Allergy, 58(2), pp. 132-8.
    Hansen KS, et al. Roasted Hazelnuts--allergenic Activity Evaluated By Double-blind, Placebo-controlled Food Challenge. Allergy. 2003;58(2):132-8. PubMed PMID: 12622744.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Roasted hazelnuts--allergenic activity evaluated by double-blind, placebo-controlled food challenge. AU - Hansen,K Skamstrup, AU - Ballmer-Weber,B K, AU - Lüttkopf,D, AU - Skov,P S, AU - Wüthrich,B, AU - Bindslev-Jensen,C, AU - Vieths,S, AU - Poulsen,L K, PY - 2003/3/8/pubmed PY - 2003/7/26/medline PY - 2003/3/8/entrez SP - 132 EP - 8 JF - Allergy JO - Allergy VL - 58 IS - 2 N2 - BACKGROUND: Allergy to hazelnuts is a common example of birch pollen related food allergy. Symptoms upon ingestion are often confined to the mouth and throat, but severe systemic reactions have been described in some patients. The aim of the study was to evaluate the reduction in allergenicity by roasting of the nuts. METHODS: Double-blind, placebo-controlled food challenges (DBPCFC) with roasted hazelnuts (140 degrees C, 40 min) were performed in 17 birch pollen allergic patients with DBPCFC-confirmed food allergy to raw hazelnuts. The effect of roasting was further evaluated by skin prick test (SPT), histamine release (HR), measurement of specific IgE, and IgE-inhibition experiments. RESULTS: In 5/17 patients the DBPCFC with the roasted nuts were positive. The symptoms were generally mild and included OAS (oral allergy syndrome) in all patients. Roasting of the nuts significantly reduced the allergenic activity evaluated by SPT, HR, specific IgE, and IgE-inhibition. Immunoblotting experiments with recombinant hazelnut allergens showed sensitization against Cor a 1.04 in 16/17 patients and against Cor a 2 in 7/17 patients. None of the patients were sensitized to Cor a 8. Challenge-positive patients did not differ from the rest in IgE-binding pattern. CONCLUSIONS: All the applied methods indicated that roasting of hazelnuts reduces the allergenicity, but since 5/17 birch pollen allergic patients were DBPCFC-positive to the roasted nuts, ingestion of roasted hazelnuts or products containing roasted hazelnuts can not be considered safe for a number of hazelnut allergic consumers. For patients with a history of severe allergic symptoms upon ingestion of hazelnuts, thorough and conscientious food labelling of hazelnuts and hazelnut residues is essential. SN - 0105-4538 UR - https://www.unboundmedicine.com/medline/citation/12622744/Roasted_hazelnuts__allergenic_activity_evaluated_by_double_blind_placebo_controlled_food_challenge_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0105-4538&date=2003&volume=58&issue=2&spage=132 DB - PRIME DP - Unbound Medicine ER -