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Genotype identification of Orientia tsutsugamushi isolated from Nan Peng Lie Islands in China.
Chin Med J (Engl). 2002 Dec; 115(12):1881-2.CM

Abstract

OBJECTIVE

To identify genotype of eight strains of Orientia tsutsugamushi (O. tsutsugamushi) isolated from Nan Peng Lie Islands in China and establish tsutsugamushi disease nature foci for this region.

METHODS

The nested polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) were used. Three primers were selected from the DNA sequence of the gene encoding type-specific 56-kDa protein of the Karp strain. The positive products were digested by Hine II and Pst I, meanwhile profiles specific to each strain were analyzed.

RESULTS

Three genotypes of O. tsutsugamushi including Karp, Kato and a new strain existed on Nan Peng Lie Islands.

CONCLUSION

Nan Peng Lie Islands is tsutsugamushi disease nature foci.

Authors+Show Affiliations

Peng G
Department of Epidemiology, Medical Institute, Guangzhou Command PLA, Guangzhou 510507, China.
Wang Z
No affiliation info available
Wang S
No affiliation info available
Huang J
No affiliation info available
Jiang P
No affiliation info available
Zeng N
No affiliation info available
Liu J
No affiliation info available
Zhu S
No affiliation info available

MeSH

AnimalsChinaDNA, BacterialGenotypeMiceOrientia tsutsugamushiPolymorphism, Restriction Fragment LengthRats

Pub Type(s)

Journal Article

Language

eng

PubMed ID

12622944

Citation

Peng, Guifu, et al. "Genotype Identification of Orientia Tsutsugamushi Isolated From Nan Peng Lie Islands in China." Chinese Medical Journal, vol. 115, no. 12, 2002, pp. 1881-2.
Peng G, Wang Z, Wang S, et al. Genotype identification of Orientia tsutsugamushi isolated from Nan Peng Lie Islands in China. Chin Med J (Engl). 2002;115(12):1881-2.
Peng, G., Wang, Z., Wang, S., Huang, J., Jiang, P., Zeng, N., Liu, J., & Zhu, S. (2002). Genotype identification of Orientia tsutsugamushi isolated from Nan Peng Lie Islands in China. Chinese Medical Journal, 115(12), 1881-2.
Peng G, et al. Genotype Identification of Orientia Tsutsugamushi Isolated From Nan Peng Lie Islands in China. Chin Med J (Engl). 2002;115(12):1881-2. PubMed PMID: 12622944.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genotype identification of Orientia tsutsugamushi isolated from Nan Peng Lie Islands in China. AU - Peng,Guifu, AU - Wang,Zhibing, AU - Wang,Shanshan, AU - Huang,Jialiang, AU - Jiang,Pulin, AU - Zeng,Nianhua, AU - Liu,Jinhua, AU - Zhu,Shaofan, PY - 2003/3/8/pubmed PY - 2003/4/1/medline PY - 2003/3/8/entrez SP - 1881 EP - 2 JF - Chinese medical journal JO - Chin Med J (Engl) VL - 115 IS - 12 N2 - OBJECTIVE: To identify genotype of eight strains of Orientia tsutsugamushi (O. tsutsugamushi) isolated from Nan Peng Lie Islands in China and establish tsutsugamushi disease nature foci for this region. METHODS: The nested polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) were used. Three primers were selected from the DNA sequence of the gene encoding type-specific 56-kDa protein of the Karp strain. The positive products were digested by Hine II and Pst I, meanwhile profiles specific to each strain were analyzed. RESULTS: Three genotypes of O. tsutsugamushi including Karp, Kato and a new strain existed on Nan Peng Lie Islands. CONCLUSION: Nan Peng Lie Islands is tsutsugamushi disease nature foci. SN - 0366-6999 UR - https://www.unboundmedicine.com/medline/citation/12622944/Genotype_identification_of_Orientia_tsutsugamushi_isolated_from_Nan_Peng_Lie_Islands_in_China_ DB - PRIME DP - Unbound Medicine ER -