Tags

Type your tag names separated by a space and hit enter

Proteome analysis of recombinant xylose-fermenting Saccharomyces cerevisiae.
Yeast. 2003 Mar; 20(4):295-314.Y

Abstract

Introduction of an active xylose utilization pathway into Saccharomyces cerevisiae, which does not naturally ferment pentose sugars, is likely to have a major impact on the overall cellular metabolism as the carbon introduced to the cells will now flow through the pentose phosphate pathway. The metabolic responses in the recombinant xylose-fermenting S. cerevisiae were studied at the proteome level by comparative two-dimensional gel electrophoresis of cellular proteins within a pH range of 3-10. Glucose-limited chemostat cultivations and corresponding chemostat cultivations performed in media containing xylose as the major carbon source were compared. The cultivations were studied in aerobic and anaerobic metabolic steady states and in addition at time points 5, 30 and 60 min after the switch-off of oxygen supply. We identified 22 proteins having a significant abundance difference on xylose compared to glucose, and 12 proteins that responded to change from aerobic to anaerobic conditions on both carbon sources. On xylose in all conditions studied, major changes were seen in the abundance of alcohol dehydrogenase 2 (Adh2p), acetaldehyde dehydrogenases 4 and 6 (Ald4p and Ald6p), and DL-glycerol 3-phosphatase (Gpp1p). Our results give indications of altered metabolic fluxes especially in the acetate and glycerol pathways in cells growing on xylose compared to glucose.

Authors+Show Affiliations

Salusjärvi L
VTT Biotechnology, PO Box 1500, FIN-02044 VTT, Finland. laura.salusjarvi@vtt.fi
Poutanen M
No affiliation info available
Pitkänen JP
No affiliation info available
Koivistoinen H
No affiliation info available
Aristidou A
No affiliation info available
Kalkkinen N
No affiliation info available
Ruohonen L
No affiliation info available
Penttilä M
No affiliation info available

MeSH

AerobiosisAnaerobiosisBioreactorsElectrophoresis, Gel, Two-DimensionalFermentationFungal ProteinsGene Expression Regulation, FungalGlucoseImage Processing, Computer-AssistedIndustrial MicrobiologyOxidation-ReductionPentose Phosphate PathwayProteomeSaccharomyces cerevisiaeSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationXylose

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12627397

Citation

Salusjärvi, Laura, et al. "Proteome Analysis of Recombinant Xylose-fermenting Saccharomyces Cerevisiae." Yeast (Chichester, England), vol. 20, no. 4, 2003, pp. 295-314.
Salusjärvi L, Poutanen M, Pitkänen JP, et al. Proteome analysis of recombinant xylose-fermenting Saccharomyces cerevisiae. Yeast. 2003;20(4):295-314.
Salusjärvi, L., Poutanen, M., Pitkänen, J. P., Koivistoinen, H., Aristidou, A., Kalkkinen, N., Ruohonen, L., & Penttilä, M. (2003). Proteome analysis of recombinant xylose-fermenting Saccharomyces cerevisiae. Yeast (Chichester, England), 20(4), 295-314.
Salusjärvi L, et al. Proteome Analysis of Recombinant Xylose-fermenting Saccharomyces Cerevisiae. Yeast. 2003;20(4):295-314. PubMed PMID: 12627397.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Proteome analysis of recombinant xylose-fermenting Saccharomyces cerevisiae. AU - Salusjärvi,Laura, AU - Poutanen,Marjo, AU - Pitkänen,Juha-Pekka, AU - Koivistoinen,Heini, AU - Aristidou,Aristos, AU - Kalkkinen,Nisse, AU - Ruohonen,Laura, AU - Penttilä,Merja, PY - 2003/3/11/pubmed PY - 2003/5/3/medline PY - 2003/3/11/entrez SP - 295 EP - 314 JF - Yeast (Chichester, England) JO - Yeast VL - 20 IS - 4 N2 - Introduction of an active xylose utilization pathway into Saccharomyces cerevisiae, which does not naturally ferment pentose sugars, is likely to have a major impact on the overall cellular metabolism as the carbon introduced to the cells will now flow through the pentose phosphate pathway. The metabolic responses in the recombinant xylose-fermenting S. cerevisiae were studied at the proteome level by comparative two-dimensional gel electrophoresis of cellular proteins within a pH range of 3-10. Glucose-limited chemostat cultivations and corresponding chemostat cultivations performed in media containing xylose as the major carbon source were compared. The cultivations were studied in aerobic and anaerobic metabolic steady states and in addition at time points 5, 30 and 60 min after the switch-off of oxygen supply. We identified 22 proteins having a significant abundance difference on xylose compared to glucose, and 12 proteins that responded to change from aerobic to anaerobic conditions on both carbon sources. On xylose in all conditions studied, major changes were seen in the abundance of alcohol dehydrogenase 2 (Adh2p), acetaldehyde dehydrogenases 4 and 6 (Ald4p and Ald6p), and DL-glycerol 3-phosphatase (Gpp1p). Our results give indications of altered metabolic fluxes especially in the acetate and glycerol pathways in cells growing on xylose compared to glucose. SN - 0749-503X UR - https://www.unboundmedicine.com/medline/citation/12627397/Proteome_analysis_of_recombinant_xylose_fermenting_Saccharomyces_cerevisiae_ DB - PRIME DP - Unbound Medicine ER -