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Skeletal muscle repair by adult human mesenchymal stem cells from synovial membrane.
J Cell Biol. 2003 Mar 17; 160(6):909-18.JC

Abstract

We have demonstrated previously that adult human synovial membrane-derived mesenchymal stem cells (hSM-MSCs) have myogenic potential in vitro (De Bari, C., F. Dell'Accio, P. Tylzanowski, and F.P. Luyten. 2001. Arthritis Rheum. 44:1928-1942). In the present study, we have characterized their myogenic differentiation in a nude mouse model of skeletal muscle regeneration and provide proof of principle of their potential use for muscle repair in the mdx mouse model of Duchenne muscular dystrophy. When implanted into regenerating nude mouse muscle, hSM-MSCs contributed to myofibers and to long term persisting functional satellite cells. No nuclear fusion hybrids were observed between donor human cells and host mouse muscle cells. Myogenic differentiation proceeded through a molecular cascade resembling embryonic muscle development. Differentiation was sensitive to environmental cues, since hSM-MSCs injected into the bloodstream engrafted in several tissues, but acquired the muscle phenotype only within skeletal muscle. When administered into dystrophic muscles of immunosuppressed mdx mice, hSM-MSCs restored sarcolemmal expression of dystrophin, reduced central nucleation, and rescued the expression of mouse mechano growth factor.

Authors+Show Affiliations

Laboratory for Skeletal Development and Joint Disorders, Dept. of Rheumatology, Katholieke Universiteit Leuven, Herestraat 49, 3000 Leuven, Belgium.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12629053

Citation

De Bari, Cosimo, et al. "Skeletal Muscle Repair By Adult Human Mesenchymal Stem Cells From Synovial Membrane." The Journal of Cell Biology, vol. 160, no. 6, 2003, pp. 909-18.
De Bari C, Dell'Accio F, Vandenabeele F, et al. Skeletal muscle repair by adult human mesenchymal stem cells from synovial membrane. J Cell Biol. 2003;160(6):909-18.
De Bari, C., Dell'Accio, F., Vandenabeele, F., Vermeesch, J. R., Raymackers, J. M., & Luyten, F. P. (2003). Skeletal muscle repair by adult human mesenchymal stem cells from synovial membrane. The Journal of Cell Biology, 160(6), 909-18.
De Bari C, et al. Skeletal Muscle Repair By Adult Human Mesenchymal Stem Cells From Synovial Membrane. J Cell Biol. 2003 Mar 17;160(6):909-18. PubMed PMID: 12629053.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Skeletal muscle repair by adult human mesenchymal stem cells from synovial membrane. AU - De Bari,Cosimo, AU - Dell'Accio,Francesco, AU - Vandenabeele,Frank, AU - Vermeesch,Joris R, AU - Raymackers,Jean-Marc, AU - Luyten,Frank P, Y1 - 2003/03/10/ PY - 2003/3/12/pubmed PY - 2003/5/17/medline PY - 2003/3/12/entrez SP - 909 EP - 18 JF - The Journal of cell biology JO - J. Cell Biol. VL - 160 IS - 6 N2 - We have demonstrated previously that adult human synovial membrane-derived mesenchymal stem cells (hSM-MSCs) have myogenic potential in vitro (De Bari, C., F. Dell'Accio, P. Tylzanowski, and F.P. Luyten. 2001. Arthritis Rheum. 44:1928-1942). In the present study, we have characterized their myogenic differentiation in a nude mouse model of skeletal muscle regeneration and provide proof of principle of their potential use for muscle repair in the mdx mouse model of Duchenne muscular dystrophy. When implanted into regenerating nude mouse muscle, hSM-MSCs contributed to myofibers and to long term persisting functional satellite cells. No nuclear fusion hybrids were observed between donor human cells and host mouse muscle cells. Myogenic differentiation proceeded through a molecular cascade resembling embryonic muscle development. Differentiation was sensitive to environmental cues, since hSM-MSCs injected into the bloodstream engrafted in several tissues, but acquired the muscle phenotype only within skeletal muscle. When administered into dystrophic muscles of immunosuppressed mdx mice, hSM-MSCs restored sarcolemmal expression of dystrophin, reduced central nucleation, and rescued the expression of mouse mechano growth factor. SN - 0021-9525 UR - https://www.unboundmedicine.com/medline/citation/12629053/Skeletal_muscle_repair_by_adult_human_mesenchymal_stem_cells_from_synovial_membrane_ L2 - https://rupress.org/jcb/article-lookup/doi/10.1083/jcb.200212064 DB - PRIME DP - Unbound Medicine ER -