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Molecular and serological aspects of HBsAg-negative hepatitis B virus infections in North America.
J Med Virol 2003; 70(1):20-6JM

Abstract

A few hepatitis B virus (HBV) infections are characterized by the presence of HBV DNA in serum or liver tissue, or both, in the absence of detectable hepatitis B surface antigen (HBsAg) in serum. However, such infections have rarely been described previously in North American patients. In the present study, 31 hepatocellular carcinoma (HCC) patients from the United States and Canada who had no detectable HBsAg in their serum were studied. In these 31 HBsAg-negative HCC patients, HBV DNA was detected in HCC and/or in adjacent nontumorous liver tissue using nested polymerase chain reaction (PCR) in 5/9 (56%) patients from the United States and in 12/22 (55%) from Canada. The 17 HBV DNA-positive/HBsAg-negative patients from the United States and Canada included 9 without any serological markers for HBV and 8 with detectable antibodies to hepatitis B core antigen. In these patients, HBV genotype C was the most prevalent genotype (11/17; 64%). HBV genotypes have not been previously reported in HCC patients from North America. Replicative intermediate forms of HBV (covalently closed circular HBV DNA) were detected in 2/17 (12%) HBV DNA-positive/HBsAg-negative patients, indicating that at least two of these patients had actively replicating HBV infections. The use of tests to detect HBV DNA permitted the identification of HBV infections in HBsAg-negative HCC patients from North America. Among these patients, those with antibody to hepatitis C virus (HCV) would otherwise have been designated "HCV-associated HCCs" based on serological tests alone. These findings provide a new perspective on determining the possible viral etiologies of HCCs in North America.

Authors+Show Affiliations

Division of Emerging and Transfusion Transmitted Diseases, Office of Blood Research and Review, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

12629639

Citation

Hsia, C C., et al. "Molecular and Serological Aspects of HBsAg-negative Hepatitis B Virus Infections in North America." Journal of Medical Virology, vol. 70, no. 1, 2003, pp. 20-6.
Hsia CC, Scudamore CH, Di Bisceglie AM, et al. Molecular and serological aspects of HBsAg-negative hepatitis B virus infections in North America. J Med Virol. 2003;70(1):20-6.
Hsia, C. C., Scudamore, C. H., Di Bisceglie, A. M., & Tabor, E. (2003). Molecular and serological aspects of HBsAg-negative hepatitis B virus infections in North America. Journal of Medical Virology, 70(1), pp. 20-6.
Hsia CC, et al. Molecular and Serological Aspects of HBsAg-negative Hepatitis B Virus Infections in North America. J Med Virol. 2003;70(1):20-6. PubMed PMID: 12629639.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular and serological aspects of HBsAg-negative hepatitis B virus infections in North America. AU - Hsia,C C, AU - Scudamore,C H, AU - Di Bisceglie,A M, AU - Tabor,E, PY - 2003/3/12/pubmed PY - 2003/5/31/medline PY - 2003/3/12/entrez SP - 20 EP - 6 JF - Journal of medical virology JO - J. Med. Virol. VL - 70 IS - 1 N2 - A few hepatitis B virus (HBV) infections are characterized by the presence of HBV DNA in serum or liver tissue, or both, in the absence of detectable hepatitis B surface antigen (HBsAg) in serum. However, such infections have rarely been described previously in North American patients. In the present study, 31 hepatocellular carcinoma (HCC) patients from the United States and Canada who had no detectable HBsAg in their serum were studied. In these 31 HBsAg-negative HCC patients, HBV DNA was detected in HCC and/or in adjacent nontumorous liver tissue using nested polymerase chain reaction (PCR) in 5/9 (56%) patients from the United States and in 12/22 (55%) from Canada. The 17 HBV DNA-positive/HBsAg-negative patients from the United States and Canada included 9 without any serological markers for HBV and 8 with detectable antibodies to hepatitis B core antigen. In these patients, HBV genotype C was the most prevalent genotype (11/17; 64%). HBV genotypes have not been previously reported in HCC patients from North America. Replicative intermediate forms of HBV (covalently closed circular HBV DNA) were detected in 2/17 (12%) HBV DNA-positive/HBsAg-negative patients, indicating that at least two of these patients had actively replicating HBV infections. The use of tests to detect HBV DNA permitted the identification of HBV infections in HBsAg-negative HCC patients from North America. Among these patients, those with antibody to hepatitis C virus (HCV) would otherwise have been designated "HCV-associated HCCs" based on serological tests alone. These findings provide a new perspective on determining the possible viral etiologies of HCCs in North America. SN - 0146-6615 UR - https://www.unboundmedicine.com/medline/citation/12629639/Molecular_and_serological_aspects_of_HBsAg_negative_hepatitis_B_virus_infections_in_North_America_ L2 - https://doi.org/10.1002/jmv.10353 DB - PRIME DP - Unbound Medicine ER -