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Thermosensitive phenotype of Escherichia coli mutant lacking NADP+-dependent isocitrate dehydrogenase.
Redox Rep. 2003; 8(1):51-6.RR

Abstract

Heat shock may increase oxidative stress due to increased production of reactive oxygen species and/or the promotion of cellular oxidation events. NADP(+)-dependent isocitrate dehydrogenase (ICDH) in Escherichia coli produces NADPH, an essential reducing equivalent for the antioxidant system. The protective role of ICDH against heat shock in E. coli was investigated in wild-type and ICDH-deficient strains. Upon exposure to heat shock, the viability was lower and the protein oxidation was higher in mutant cells as compared to wild-type cells. Induction and inactivation of antioxidant enzymes were observed after their exposure to heat shock both in wild-type and in mutant cells. However, wild-type cells maintained significantly higher activities of antioxidant enzymes than did mutant cells. These results suggest that ICDH plays an important role as an antioxidant enzyme in cellular defense against heat shock through the removal of reactive oxygen species as well as in the protection of other antioxidant enzymes.

Authors+Show Affiliations

Department of Biochemistry, College of Natural Sciences, Kyungpook National University, Taegu, Korea.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12631445

Citation

Choi, In Youl, et al. "Thermosensitive Phenotype of Escherichia Coli Mutant Lacking NADP+-dependent Isocitrate Dehydrogenase." Redox Report : Communications in Free Radical Research, vol. 8, no. 1, 2003, pp. 51-6.
Choi IY, Sup KI, Kim HJ, et al. Thermosensitive phenotype of Escherichia coli mutant lacking NADP+-dependent isocitrate dehydrogenase. Redox Rep. 2003;8(1):51-6.
Choi, I. Y., Sup, K. I., Kim, H. J., & Park, J. W. (2003). Thermosensitive phenotype of Escherichia coli mutant lacking NADP+-dependent isocitrate dehydrogenase. Redox Report : Communications in Free Radical Research, 8(1), 51-6.
Choi IY, et al. Thermosensitive Phenotype of Escherichia Coli Mutant Lacking NADP+-dependent Isocitrate Dehydrogenase. Redox Rep. 2003;8(1):51-6. PubMed PMID: 12631445.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Thermosensitive phenotype of Escherichia coli mutant lacking NADP+-dependent isocitrate dehydrogenase. AU - Choi,In Youl, AU - Sup,Kil In, AU - Kim,Hyun Jeong, AU - Park,Jeen-Woo, PY - 2003/3/13/pubmed PY - 2003/9/13/medline PY - 2003/3/13/entrez SP - 51 EP - 6 JF - Redox report : communications in free radical research JO - Redox Rep VL - 8 IS - 1 N2 - Heat shock may increase oxidative stress due to increased production of reactive oxygen species and/or the promotion of cellular oxidation events. NADP(+)-dependent isocitrate dehydrogenase (ICDH) in Escherichia coli produces NADPH, an essential reducing equivalent for the antioxidant system. The protective role of ICDH against heat shock in E. coli was investigated in wild-type and ICDH-deficient strains. Upon exposure to heat shock, the viability was lower and the protein oxidation was higher in mutant cells as compared to wild-type cells. Induction and inactivation of antioxidant enzymes were observed after their exposure to heat shock both in wild-type and in mutant cells. However, wild-type cells maintained significantly higher activities of antioxidant enzymes than did mutant cells. These results suggest that ICDH plays an important role as an antioxidant enzyme in cellular defense against heat shock through the removal of reactive oxygen species as well as in the protection of other antioxidant enzymes. SN - 1351-0002 UR - https://www.unboundmedicine.com/medline/citation/12631445/Thermosensitive_phenotype_of_Escherichia_coli_mutant_lacking_NADP+_dependent_isocitrate_dehydrogenase_ L2 - https://www.tandfonline.com/doi/full/10.1179/135100003125001251 DB - PRIME DP - Unbound Medicine ER -