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Localization of postresection EGF receptor expression using laser capture microdissection.
J Pediatr Surg. 2003 Mar; 38(3):440-5.JP

Abstract

BACKGROUND/PURPOSE

Epidermal growth factor (EGF) and its receptor (EGFR) are key components in the genesis of adaptation after small bowel resection (SBR). Within intestinal homogenates, EGFR expression is increased after SBR; however, the exact cells responsible for altered EGFR expression are unknown. In this study, laser capture microdissection (LCM) microscopy was used to elucidate the specific cellular compartment(s) responsible for postresection changes in EGFR expression.

METHODS

Male ICR mice underwent a 50% proximal SBR or sham operation. After 3 days, frozen sections were taken from the remnant ileum. Individual cells from villi, crypt, muscularis, and mesenchymal compartments were isolated by LCM. EGFR mRNA expression for each cell compartment was quantified using real-time polymerase chain reaction (PCR).

RESULTS

EGFR expression was increased after SBR within the crypt (2-fold) and muscularis compartments (3-fold). There were no changes detected after SBR in the villus tips or mesenchymal compartments.

CONCLUSIONS

Increased expression of EGFR in crypts directly correlates with the zone of cell proliferation and supports the hypothesis that EGFR signaling is crucial for the mitogenic stimulus for adaptation. The finding of increased EGFR expression in the muscular compartment is novel and may implicate a role for EGFR as a mediator of the muscular hyperplasia seen after massive SBR.

Authors+Show Affiliations

Knott AW
Division of Pediatric Surgery, Cincinnati Children's Hospital Medical Center, and the Department of Surgery, University of Cincinnati College of Medicine, Cincinnati, Ohio 45229-3039, USA.
Erwin CR
No affiliation info available
Profitt SA
No affiliation info available
Juno RJ
No affiliation info available
Warner BW
No affiliation info available

MeSH

Adaptation, PhysiologicalAnimalsCell SeparationComputer SystemsErbB ReceptorsFrozen SectionsHistocytological Preparation TechniquesIleumIntestinal MucosaLasersMaleMiceMice, Inbred ICRMicrovilliMuscle, SmoothPolymerase Chain ReactionRNA, MessengerShort Bowel Syndrome

Pub Type(s)

Comparative Study
Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

12632364

Citation

Knott, Andrew W., et al. "Localization of Postresection EGF Receptor Expression Using Laser Capture Microdissection." Journal of Pediatric Surgery, vol. 38, no. 3, 2003, pp. 440-5.
Knott AW, Erwin CR, Profitt SA, et al. Localization of postresection EGF receptor expression using laser capture microdissection. J Pediatr Surg. 2003;38(3):440-5.
Knott, A. W., Erwin, C. R., Profitt, S. A., Juno, R. J., & Warner, B. W. (2003). Localization of postresection EGF receptor expression using laser capture microdissection. Journal of Pediatric Surgery, 38(3), 440-5.
Knott AW, et al. Localization of Postresection EGF Receptor Expression Using Laser Capture Microdissection. J Pediatr Surg. 2003;38(3):440-5. PubMed PMID: 12632364.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Localization of postresection EGF receptor expression using laser capture microdissection. AU - Knott,Andrew W, AU - Erwin,Christopher R, AU - Profitt,Sherri A, AU - Juno,Russell J, AU - Warner,Brad W, PY - 2003/3/13/pubmed PY - 2003/11/6/medline PY - 2003/3/13/entrez SP - 440 EP - 5 JF - Journal of pediatric surgery JO - J Pediatr Surg VL - 38 IS - 3 N2 - BACKGROUND/PURPOSE: Epidermal growth factor (EGF) and its receptor (EGFR) are key components in the genesis of adaptation after small bowel resection (SBR). Within intestinal homogenates, EGFR expression is increased after SBR; however, the exact cells responsible for altered EGFR expression are unknown. In this study, laser capture microdissection (LCM) microscopy was used to elucidate the specific cellular compartment(s) responsible for postresection changes in EGFR expression. METHODS: Male ICR mice underwent a 50% proximal SBR or sham operation. After 3 days, frozen sections were taken from the remnant ileum. Individual cells from villi, crypt, muscularis, and mesenchymal compartments were isolated by LCM. EGFR mRNA expression for each cell compartment was quantified using real-time polymerase chain reaction (PCR). RESULTS: EGFR expression was increased after SBR within the crypt (2-fold) and muscularis compartments (3-fold). There were no changes detected after SBR in the villus tips or mesenchymal compartments. CONCLUSIONS: Increased expression of EGFR in crypts directly correlates with the zone of cell proliferation and supports the hypothesis that EGFR signaling is crucial for the mitogenic stimulus for adaptation. The finding of increased EGFR expression in the muscular compartment is novel and may implicate a role for EGFR as a mediator of the muscular hyperplasia seen after massive SBR. SN - 1531-5037 UR - https://www.unboundmedicine.com/medline/citation/12632364/Localization_of_postresection_EGF_receptor_expression_using_laser_capture_microdissection_ DB - PRIME DP - Unbound Medicine ER -