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Sequencing of 16S rDNA of Klebsiella: taxonomic relations within the genus and to other Enterobacteriaceae.
Int J Med Microbiol. 2003 Feb; 292(7-8):495-503.IJ

Abstract

The 16S rDNAs of 20 strains of Klebsiella were sequenced and used for construction of a phylogenetic tree together with already published Enterobacteriaceae 16S rDNA sequences. The taxonomy within the Klebsiella genus, as reflected by the 16S rDNA tree, was in agreement with existing DNA-DNA hybridisation and numerical taxonomy data, indicating that for Klebsiella, 16S rDNA sequencing is a valid method for identification and taxonomical purposes. Five closely related clusters were found in the Klebsiella genus; Cluster I, K. oxytoca; Cluster II, K. terrigena, Cluster III, K. planticola and K. ornithinolytica; Cluster IV, Enterobacter aerogenes (K. mobilis); and Cluster V, K. pneumoniae. The position of Calymmatobacterium granulomatis within the genus and closest to K. pneumoniae was confirmed. For the species K. oxytoca, data seem to indicate a subdivision into two subspecies. In addition, a biochemically aberrant Klebsiella strain (BEC441) that was included in the analysis could not be assigned to any of the known species, but was found to be closest related to K. oxytoca. Furthermore, the high sequence similarity between the two environmental species K. planticola and K. ornithinolytica does not justify a distinction of the two species. Finally, within a 165-bp stretch of the 16S rDNA sequences, species-specific nucleotides were found.

Authors+Show Affiliations

Department of Clinical Biochemistry, Statens Serum Institut, Copenhagen, Denmark.No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

12635932

Citation

Boye, Kit, and Dennis S. Hansen. "Sequencing of 16S rDNA of Klebsiella: Taxonomic Relations Within the Genus and to Other Enterobacteriaceae." International Journal of Medical Microbiology : IJMM, vol. 292, no. 7-8, 2003, pp. 495-503.
Boye K, Hansen DS. Sequencing of 16S rDNA of Klebsiella: taxonomic relations within the genus and to other Enterobacteriaceae. Int J Med Microbiol. 2003;292(7-8):495-503.
Boye, K., & Hansen, D. S. (2003). Sequencing of 16S rDNA of Klebsiella: taxonomic relations within the genus and to other Enterobacteriaceae. International Journal of Medical Microbiology : IJMM, 292(7-8), 495-503.
Boye K, Hansen DS. Sequencing of 16S rDNA of Klebsiella: Taxonomic Relations Within the Genus and to Other Enterobacteriaceae. Int J Med Microbiol. 2003;292(7-8):495-503. PubMed PMID: 12635932.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sequencing of 16S rDNA of Klebsiella: taxonomic relations within the genus and to other Enterobacteriaceae. AU - Boye,Kit, AU - Hansen,Dennis S, PY - 2003/3/15/pubmed PY - 2003/4/16/medline PY - 2003/3/15/entrez SP - 495 EP - 503 JF - International journal of medical microbiology : IJMM JO - Int J Med Microbiol VL - 292 IS - 7-8 N2 - The 16S rDNAs of 20 strains of Klebsiella were sequenced and used for construction of a phylogenetic tree together with already published Enterobacteriaceae 16S rDNA sequences. The taxonomy within the Klebsiella genus, as reflected by the 16S rDNA tree, was in agreement with existing DNA-DNA hybridisation and numerical taxonomy data, indicating that for Klebsiella, 16S rDNA sequencing is a valid method for identification and taxonomical purposes. Five closely related clusters were found in the Klebsiella genus; Cluster I, K. oxytoca; Cluster II, K. terrigena, Cluster III, K. planticola and K. ornithinolytica; Cluster IV, Enterobacter aerogenes (K. mobilis); and Cluster V, K. pneumoniae. The position of Calymmatobacterium granulomatis within the genus and closest to K. pneumoniae was confirmed. For the species K. oxytoca, data seem to indicate a subdivision into two subspecies. In addition, a biochemically aberrant Klebsiella strain (BEC441) that was included in the analysis could not be assigned to any of the known species, but was found to be closest related to K. oxytoca. Furthermore, the high sequence similarity between the two environmental species K. planticola and K. ornithinolytica does not justify a distinction of the two species. Finally, within a 165-bp stretch of the 16S rDNA sequences, species-specific nucleotides were found. SN - 1438-4221 UR - https://www.unboundmedicine.com/medline/citation/12635932/Sequencing_of_16S_rDNA_of_Klebsiella:_taxonomic_relations_within_the_genus_and_to_other_Enterobacteriaceae. L2 - https://linkinghub.elsevier.com/retrieve/pii/S1438-4221(04)70126-5 DB - PRIME DP - Unbound Medicine ER -