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Indefinite islet protection from autoimmune destruction in nonobese diabetic mice by agarose microencapsulation without immunosuppression.
Transplantation. 2003 Mar 15; 75(5):619-25.T

Abstract

BACKGROUND

The recurrence of autoimmunity and allograft rejection act as major barriers to the widespread use of islet transplantation as a cure for type 1 diabetes. The aim of this study was to evaluate the feasibility of immunoisolation by use of an agarose microcapsule to prevent autoimmune recurrence after islet transplantation.

METHODS

Highly purified islets were isolated from 6- to 8-week-old prediabetic male nonobese diabetic (NOD) mice and microencapsulated in 5% agarose hydrogel as a semipermeable membrane. Islet function was evaluated by a syngeneic islet transplantation model, in which islets were transplanted into spontaneously diabetic NOD mice.

RESULTS

The nonencapsulated islet grafts were destroyed and diabetes recurred within 2 weeks after transplantation in all 12 mice. In contrast, 13 of the 16 mice that underwent transplantation with microencapsulated islets maintained normoglycemia for more than 100 days after islet transplantation. Histologic examination of the nonencapsulated islet grafts showed massive mononuclear cellular infiltration with beta-cell destruction. In contrast, the microencapsulated islets showed well-granulated beta cells with no mononuclear cellular infiltration around the microcapsules or in the accompanying blood capillaries between the microcapsules.

CONCLUSIONS

Agarose microcapsules were able to completely protect NOD islet isografts from autoimmune destruction in the syngeneic islet transplantation model.

Authors+Show Affiliations

First Department of Surgery, Nara Medical University, Kashihara, Nara, Japan. tsunehirokobayashi@hotmail.com.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12640299

Citation

Kobayashi, Tsunehiro, et al. "Indefinite Islet Protection From Autoimmune Destruction in Nonobese Diabetic Mice By Agarose Microencapsulation Without Immunosuppression." Transplantation, vol. 75, no. 5, 2003, pp. 619-25.
Kobayashi T, Aomatsu Y, Iwata H, et al. Indefinite islet protection from autoimmune destruction in nonobese diabetic mice by agarose microencapsulation without immunosuppression. Transplantation. 2003;75(5):619-25.
Kobayashi, T., Aomatsu, Y., Iwata, H., Kin, T., Kanehiro, H., Hisanaga, M., Ko, S., Nagao, M., & Nakajima, Y. (2003). Indefinite islet protection from autoimmune destruction in nonobese diabetic mice by agarose microencapsulation without immunosuppression. Transplantation, 75(5), 619-25.
Kobayashi T, et al. Indefinite Islet Protection From Autoimmune Destruction in Nonobese Diabetic Mice By Agarose Microencapsulation Without Immunosuppression. Transplantation. 2003 Mar 15;75(5):619-25. PubMed PMID: 12640299.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Indefinite islet protection from autoimmune destruction in nonobese diabetic mice by agarose microencapsulation without immunosuppression. AU - Kobayashi,Tsunehiro, AU - Aomatsu,Yukio, AU - Iwata,Hiroo, AU - Kin,Tatsuya, AU - Kanehiro,Hiromichi, AU - Hisanaga,Michiyoshi, AU - Ko,Saiho, AU - Nagao,Mitsuo, AU - Nakajima,Yoshiyuki, PY - 2003/3/18/pubmed PY - 2003/4/11/medline PY - 2003/3/18/entrez SP - 619 EP - 25 JF - Transplantation JO - Transplantation VL - 75 IS - 5 N2 - BACKGROUND: The recurrence of autoimmunity and allograft rejection act as major barriers to the widespread use of islet transplantation as a cure for type 1 diabetes. The aim of this study was to evaluate the feasibility of immunoisolation by use of an agarose microcapsule to prevent autoimmune recurrence after islet transplantation. METHODS: Highly purified islets were isolated from 6- to 8-week-old prediabetic male nonobese diabetic (NOD) mice and microencapsulated in 5% agarose hydrogel as a semipermeable membrane. Islet function was evaluated by a syngeneic islet transplantation model, in which islets were transplanted into spontaneously diabetic NOD mice. RESULTS: The nonencapsulated islet grafts were destroyed and diabetes recurred within 2 weeks after transplantation in all 12 mice. In contrast, 13 of the 16 mice that underwent transplantation with microencapsulated islets maintained normoglycemia for more than 100 days after islet transplantation. Histologic examination of the nonencapsulated islet grafts showed massive mononuclear cellular infiltration with beta-cell destruction. In contrast, the microencapsulated islets showed well-granulated beta cells with no mononuclear cellular infiltration around the microcapsules or in the accompanying blood capillaries between the microcapsules. CONCLUSIONS: Agarose microcapsules were able to completely protect NOD islet isografts from autoimmune destruction in the syngeneic islet transplantation model. SN - 0041-1337 UR - https://www.unboundmedicine.com/medline/citation/12640299/Indefinite_islet_protection_from_autoimmune_destruction_in_nonobese_diabetic_mice_by_agarose_microencapsulation_without_immunosuppression_ L2 - https://doi.org/10.1097/01.TP.0000053749.36365.7E DB - PRIME DP - Unbound Medicine ER -