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[Analysis on the vacA dominant genotypes and their nucleotide sequences of Helicobacter pylori isolates in Zhejiang area].
Zhejiang Da Xue Xue Bao Yi Xue Ban. 2003 Feb; 32(1):24-8.ZD

Abstract

OBJECTIVE

To determine vacA dominant genotypes of Helicobacter pylori in patients with peptic ulcer (PU) or chronic gastritis (CG).

METHODS

H.pylori strains were isolated from mucosa samples of gastric antrum and corpus of patients suffering from PU (n=29) or CG (n=34), 126 strains of H.pylori were selected for PCR to detect s and m regions in vacA gene of the isolates. Parts of the amplification products were sequenced after T A cloning. The correlation between infection or coinfection with different vacA genotypes of H.pylori and different gastroduodenal diseases was further analyzed.

RESULTS

The positive amplification products of vacA gene, s and m regions, were found in the DNA samples of all the isolates. In these products, s1a/m1, s1a/m2, s1a/m1b and s1a/m1b m2 genotypes of vacA gene were detected and s1b and s2m1a genotypes absent. Proportions of the s1a/m1, s1a/m2, s1a/m1b and s1a/m1b-m2 genotypes were 7.1% (9/126), 61.9% (78/126), 29.4% (37/126) and 1.6% (2/126), respectively. 17.5% (11/63) of the patients were confirmed to be coinfected with different genotype H.pylori strains. No statistical differences were found in the distribution of different genotype H.pylori strain infection in the gastric diseases (P>0.05). In comparison with the reported sequences of H.pylori strain 60190 with s1a genotype and strain 87-203 with m2 genotype, homologies of the nucleotide sequences of s1a PCR products from 6 strains of H.pylori isolates and m2 PCR products from 4 strains of H.pylori isolates were 93.15% approximate, equals 94.86%and 93.63% approximate, equals 97.61%, respectively.

CONCLUSION

H.pylori with s1a/m2 or s1a/m1b are the dominant genotypes in the PU or GC patients in Zhejiang area. The nucleotide sequences of partial amplification products from the vacA dominant genotypes of H.pylori show high homology compared with the reported sequences. Part of the patients may be coinfected with different vacA genotypes of H.pylori.

Authors+Show Affiliations

Department of Medical Microbiology and Parasitology, College of Medical Sciences, Zhejiang University, Hangzhou 310031, China.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

chi

PubMed ID

12640705

Citation

Chen, Xue-Jun, et al. "[Analysis On the vacA Dominant Genotypes and Their Nucleotide Sequences of Helicobacter Pylori Isolates in Zhejiang Area]." Zhejiang Da Xue Xue Bao. Yi Xue Ban = Journal of Zhejiang University. Medical Sciences, vol. 32, no. 1, 2003, pp. 24-8.
Chen XJ, Yan J, Mao YF. [Analysis on the vacA dominant genotypes and their nucleotide sequences of Helicobacter pylori isolates in Zhejiang area]. Zhejiang Da Xue Xue Bao Yi Xue Ban. 2003;32(1):24-8.
Chen, X. J., Yan, J., & Mao, Y. F. (2003). [Analysis on the vacA dominant genotypes and their nucleotide sequences of Helicobacter pylori isolates in Zhejiang area]. Zhejiang Da Xue Xue Bao. Yi Xue Ban = Journal of Zhejiang University. Medical Sciences, 32(1), 24-8.
Chen XJ, Yan J, Mao YF. [Analysis On the vacA Dominant Genotypes and Their Nucleotide Sequences of Helicobacter Pylori Isolates in Zhejiang Area]. Zhejiang Da Xue Xue Bao Yi Xue Ban. 2003;32(1):24-8. PubMed PMID: 12640705.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Analysis on the vacA dominant genotypes and their nucleotide sequences of Helicobacter pylori isolates in Zhejiang area]. AU - Chen,Xue-Jun, AU - Yan,Jie, AU - Mao,Ya-Fei, PY - 2003/3/18/pubmed PY - 2003/4/16/medline PY - 2003/3/18/entrez SP - 24 EP - 8 JF - Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences JO - Zhejiang Da Xue Xue Bao Yi Xue Ban VL - 32 IS - 1 N2 - OBJECTIVE: To determine vacA dominant genotypes of Helicobacter pylori in patients with peptic ulcer (PU) or chronic gastritis (CG). METHODS: H.pylori strains were isolated from mucosa samples of gastric antrum and corpus of patients suffering from PU (n=29) or CG (n=34), 126 strains of H.pylori were selected for PCR to detect s and m regions in vacA gene of the isolates. Parts of the amplification products were sequenced after T A cloning. The correlation between infection or coinfection with different vacA genotypes of H.pylori and different gastroduodenal diseases was further analyzed. RESULTS: The positive amplification products of vacA gene, s and m regions, were found in the DNA samples of all the isolates. In these products, s1a/m1, s1a/m2, s1a/m1b and s1a/m1b m2 genotypes of vacA gene were detected and s1b and s2m1a genotypes absent. Proportions of the s1a/m1, s1a/m2, s1a/m1b and s1a/m1b-m2 genotypes were 7.1% (9/126), 61.9% (78/126), 29.4% (37/126) and 1.6% (2/126), respectively. 17.5% (11/63) of the patients were confirmed to be coinfected with different genotype H.pylori strains. No statistical differences were found in the distribution of different genotype H.pylori strain infection in the gastric diseases (P>0.05). In comparison with the reported sequences of H.pylori strain 60190 with s1a genotype and strain 87-203 with m2 genotype, homologies of the nucleotide sequences of s1a PCR products from 6 strains of H.pylori isolates and m2 PCR products from 4 strains of H.pylori isolates were 93.15% approximate, equals 94.86%and 93.63% approximate, equals 97.61%, respectively. CONCLUSION: H.pylori with s1a/m2 or s1a/m1b are the dominant genotypes in the PU or GC patients in Zhejiang area. The nucleotide sequences of partial amplification products from the vacA dominant genotypes of H.pylori show high homology compared with the reported sequences. Part of the patients may be coinfected with different vacA genotypes of H.pylori. SN - 1008-9292 UR - https://www.unboundmedicine.com/medline/citation/12640705/[Analysis_on_the_vacA_dominant_genotypes_and_their_nucleotide_sequences_of_Helicobacter_pylori_isolates_in_Zhejiang_area]_ DB - PRIME DP - Unbound Medicine ER -
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