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Characterization of anthocyanins in grape juices by ion trap liquid chromatography-mass spectrometry.
J Agric Food Chem. 2003 Mar 26; 51(7):1839-44.JA

Abstract

A reverse phase HPLC and electrospray interface with ion trap mass spectrometer method was developed for the characterization of anthocyanins in Concord, Rubired, and Salvador grape juices. Rubired and Salvador grapes are hybrids from Vitis vinifera and Vitis rupestris. Concord grape is a grape from the native American cultivar Vitis labrusca. Individual anthocyanins in these three varieties were identified on the basis of UV-vis and MS spectra and further elucidated by MS/MS spectra. Anthocyanins in Salvador and Concord grapes were 3-O-glucosides, 3-O-(6' '-O-p-coumaroyl)glucosides, 3-O-(6' '-O-p-acetyl)glucosides, 3,5-O-diglucosides, and 3-O-(6' '-O-p-coumaroyl)-5-O-diglucosides of delphinidin, cyanidin, petunidin, peonidin, and malvidin. Vitisin B was detected in Salvador grape juice. Anthocyanins in Rubired grape juice were primarily anthocyanin diglucosides: peonidin 3,5-O-diglucoside, malvidin 3,5-O-diglucoside, peonidin 3-O-(6' '-O-p-coumaroyl)-5-O-diglucoside, and malvidin 3-O-(6' '-O-p-coumaroyl)-5-O-diglucoside are the four major anthocyanins. The presence of pelargonidin 3-O-glucoside, not previously reported, has been established for the first time in all three juices.

Authors+Show Affiliations

Research and Development, Canandaigua Wine Company, 12667 Road 24, Madera, California 93639, USA. haibo.wang@cwine.comNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

12643639

Citation

Wang, Haibo, et al. "Characterization of Anthocyanins in Grape Juices By Ion Trap Liquid Chromatography-mass Spectrometry." Journal of Agricultural and Food Chemistry, vol. 51, no. 7, 2003, pp. 1839-44.
Wang H, Race EJ, Shrikhande AJ. Characterization of anthocyanins in grape juices by ion trap liquid chromatography-mass spectrometry. J Agric Food Chem. 2003;51(7):1839-44.
Wang, H., Race, E. J., & Shrikhande, A. J. (2003). Characterization of anthocyanins in grape juices by ion trap liquid chromatography-mass spectrometry. Journal of Agricultural and Food Chemistry, 51(7), 1839-44.
Wang H, Race EJ, Shrikhande AJ. Characterization of Anthocyanins in Grape Juices By Ion Trap Liquid Chromatography-mass Spectrometry. J Agric Food Chem. 2003 Mar 26;51(7):1839-44. PubMed PMID: 12643639.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of anthocyanins in grape juices by ion trap liquid chromatography-mass spectrometry. AU - Wang,Haibo, AU - Race,Edward J, AU - Shrikhande,Anil J, PY - 2003/3/20/pubmed PY - 2003/5/8/medline PY - 2003/3/20/entrez SP - 1839 EP - 44 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 51 IS - 7 N2 - A reverse phase HPLC and electrospray interface with ion trap mass spectrometer method was developed for the characterization of anthocyanins in Concord, Rubired, and Salvador grape juices. Rubired and Salvador grapes are hybrids from Vitis vinifera and Vitis rupestris. Concord grape is a grape from the native American cultivar Vitis labrusca. Individual anthocyanins in these three varieties were identified on the basis of UV-vis and MS spectra and further elucidated by MS/MS spectra. Anthocyanins in Salvador and Concord grapes were 3-O-glucosides, 3-O-(6' '-O-p-coumaroyl)glucosides, 3-O-(6' '-O-p-acetyl)glucosides, 3,5-O-diglucosides, and 3-O-(6' '-O-p-coumaroyl)-5-O-diglucosides of delphinidin, cyanidin, petunidin, peonidin, and malvidin. Vitisin B was detected in Salvador grape juice. Anthocyanins in Rubired grape juice were primarily anthocyanin diglucosides: peonidin 3,5-O-diglucoside, malvidin 3,5-O-diglucoside, peonidin 3-O-(6' '-O-p-coumaroyl)-5-O-diglucoside, and malvidin 3-O-(6' '-O-p-coumaroyl)-5-O-diglucoside are the four major anthocyanins. The presence of pelargonidin 3-O-glucoside, not previously reported, has been established for the first time in all three juices. SN - 0021-8561 UR - https://www.unboundmedicine.com/medline/citation/12643639/Characterization_of_anthocyanins_in_grape_juices_by_ion_trap_liquid_chromatography_mass_spectrometry_ L2 - https://doi.org/10.1021/jf0260747 DB - PRIME DP - Unbound Medicine ER -