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Localization of AT(2) receptors in the nucleus of the solitary tract of spontaneously hypertensive and Wistar Kyoto rats using [125I] CGP42112: upregulation of a non-angiotensin II binding site following unilateral nodose ganglionectomy.
Brain Res 2003; 968(1):139-55BR

Abstract

We have examined the binding distribution of a selective AT(2) receptor ligand [125I] CGP42112 in the brain of adult Wistar-Kyoto (WKY) and spontaneously hypertensive rats (SHR). AT(2) receptor localization was also examined in the rat brainstem following unilateral nodose ganglionectomy. Specific [125I] CGP42112 binding was observed in discrete brain regions from both rat strains, including the nucleus of the solitary tract (NTS), and did not differ between WKY and SHR. [125I] CGP42112 binding in the NTS revealed an AT(2) receptor component that was displaceable by PD 123319 and Ang II (50-58%), as well as a non-angiotensin II receptor component (42-49%). Following unilateral nodose ganglionectomy, [125I] CGP42112 binding density on the denervated side of the NTS was increased approximately two-fold in both WKY and SHR. This increased [125I] CGP42112 binding density in the ipsilateral NTS was comprised of a greater non-angiotensin II component than that observed in the sham groups, since only approximately 30% was displaced by PD123319 and angiotensin II. Furthermore, [125I] CGP42112 also revealed high binding density on the denervated side in the dorsal motor nucleus and the nucleus ambiguus in both WKY and SHR. AT(2) receptor immunoreactivity was also visualised in the NTS of sham operated rats, but was not observed in the dorsal motor nucleus or the nucleus ambiguus, nor was it up-regulated following nodose ganglionectomy. These results demonstrate, for the first time, an AT(2) receptor binding site in the NTS, as well as a non-angiotensin II [125I] CGP42112 binding site. These studies also demonstrate that nodose ganglionectomy represents a useful model in which to study a non-angiotensin II [125I] CGP42112 binding site that is up-regulated following degeneration of afferent vagal nerves.

Authors+Show Affiliations

Department of Pharmacology, Monash University, 3800, Victoria, Australia.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12644272

Citation

Roulston, Carli L., et al. "Localization of AT(2) Receptors in the Nucleus of the Solitary Tract of Spontaneously Hypertensive and Wistar Kyoto Rats Using [125I] CGP42112: Upregulation of a Non-angiotensin II Binding Site Following Unilateral Nodose Ganglionectomy." Brain Research, vol. 968, no. 1, 2003, pp. 139-55.
Roulston CL, Lawrence AJ, Jarrott B, et al. Localization of AT(2) receptors in the nucleus of the solitary tract of spontaneously hypertensive and Wistar Kyoto rats using [125I] CGP42112: upregulation of a non-angiotensin II binding site following unilateral nodose ganglionectomy. Brain Res. 2003;968(1):139-55.
Roulston, C. L., Lawrence, A. J., Jarrott, B., & Widdop, R. E. (2003). Localization of AT(2) receptors in the nucleus of the solitary tract of spontaneously hypertensive and Wistar Kyoto rats using [125I] CGP42112: upregulation of a non-angiotensin II binding site following unilateral nodose ganglionectomy. Brain Research, 968(1), pp. 139-55.
Roulston CL, et al. Localization of AT(2) Receptors in the Nucleus of the Solitary Tract of Spontaneously Hypertensive and Wistar Kyoto Rats Using [125I] CGP42112: Upregulation of a Non-angiotensin II Binding Site Following Unilateral Nodose Ganglionectomy. Brain Res. 2003 Apr 4;968(1):139-55. PubMed PMID: 12644272.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Localization of AT(2) receptors in the nucleus of the solitary tract of spontaneously hypertensive and Wistar Kyoto rats using [125I] CGP42112: upregulation of a non-angiotensin II binding site following unilateral nodose ganglionectomy. AU - Roulston,Carli L, AU - Lawrence,Andrew J, AU - Jarrott,Bevyn, AU - Widdop,Robert E, PY - 2003/3/20/pubmed PY - 2003/6/5/medline PY - 2003/3/20/entrez SP - 139 EP - 55 JF - Brain research JO - Brain Res. VL - 968 IS - 1 N2 - We have examined the binding distribution of a selective AT(2) receptor ligand [125I] CGP42112 in the brain of adult Wistar-Kyoto (WKY) and spontaneously hypertensive rats (SHR). AT(2) receptor localization was also examined in the rat brainstem following unilateral nodose ganglionectomy. Specific [125I] CGP42112 binding was observed in discrete brain regions from both rat strains, including the nucleus of the solitary tract (NTS), and did not differ between WKY and SHR. [125I] CGP42112 binding in the NTS revealed an AT(2) receptor component that was displaceable by PD 123319 and Ang II (50-58%), as well as a non-angiotensin II receptor component (42-49%). Following unilateral nodose ganglionectomy, [125I] CGP42112 binding density on the denervated side of the NTS was increased approximately two-fold in both WKY and SHR. This increased [125I] CGP42112 binding density in the ipsilateral NTS was comprised of a greater non-angiotensin II component than that observed in the sham groups, since only approximately 30% was displaced by PD123319 and angiotensin II. Furthermore, [125I] CGP42112 also revealed high binding density on the denervated side in the dorsal motor nucleus and the nucleus ambiguus in both WKY and SHR. AT(2) receptor immunoreactivity was also visualised in the NTS of sham operated rats, but was not observed in the dorsal motor nucleus or the nucleus ambiguus, nor was it up-regulated following nodose ganglionectomy. These results demonstrate, for the first time, an AT(2) receptor binding site in the NTS, as well as a non-angiotensin II [125I] CGP42112 binding site. These studies also demonstrate that nodose ganglionectomy represents a useful model in which to study a non-angiotensin II [125I] CGP42112 binding site that is up-regulated following degeneration of afferent vagal nerves. SN - 0006-8993 UR - https://www.unboundmedicine.com/medline/citation/12644272/Localization_of_AT_2__receptors_in_the_nucleus_of_the_solitary_tract_of_spontaneously_hypertensive_and_Wistar_Kyoto_rats_using_[125I]_CGP42112:_upregulation_of_a_non_angiotensin_II_binding_site_following_unilateral_nodose_ganglionectomy_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006899303022315 DB - PRIME DP - Unbound Medicine ER -