TY - JOUR T1 - Liquid chromatographic assay for the simultaneous determination of indole-3-carbinol and its acid condensation products in plasma. AU - Anderton,Mark J, AU - Jukes,Rebekah, AU - Lamb,John H, AU - Manson,Margaret M, AU - Gescher,Andreas, AU - Steward,William P, AU - Williams,Marion L, PY - 2003/3/26/pubmed PY - 2003/12/13/medline PY - 2003/3/26/entrez SP - 281 EP - 91 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 787 IS - 2 N2 - A high-performance liquid chromatographic method was developed for the simultaneous determination of indole-3-carbinol (I3C), 3,3'-diindolylmethane (DIM), [2-(indol-3-ylmethyl)-indol-3-yl]indol-3-ylmethane (LTr(1)), and indolo[3,2b]carbazole (ICZ). Compounds were extracted from mouse plasma using tert.-butyl methyl ether, incorporating 4-methoxy-indole as internal standard. Chromatographic separation utilized a Waters Symmetry RP18 in tandem with a Thermoquest BDS C(18) column, an acetonitrile-water gradient and UV (280 nm) in series with fluorescence (ex. 335 nm; em. 415 nm) detection. Calibration curves were linear (r(2)>0.99) between 50 and 15,000 ng/ml for I3C; 150 and 15,000 ng/ml for LTr(1); and 0.15 and 37.5 ng/ml for ICZ and the method was reproducible and precise (within-day and between-day coefficients of variation below 9.7 and 13%, respectively). The method described is suitable for comprehensive pharmacokinetic studies with indole-3-carbinol. SN - 1570-0232 UR - https://www.unboundmedicine.com/medline/citation/12650751/Liquid_chromatographic_assay_for_the_simultaneous_determination_of_indole_3_carbinol_and_its_acid_condensation_products_in_plasma_ DB - PRIME DP - Unbound Medicine ER -