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Evaluation of human IgG subclass antibodies in the serodiagnosis of angiostrongyliasis.
Parasitol Res. 2003 Apr; 89(6):425-9.PR

Abstract

Immunoglobulin G subclass antibody (IgG1, IgG2, IgG3, and IgG4) responses to the rat lungworm, Angiostrongylus cantonensis, were analyzed using the immunoblotting technique in an attempt to further improve the sensitivity and specificity for the serodiagnosis of human angiostrongyliasis. Serum samples from patients with proven angiostrongyliasis and from clinically suspected cases of angiostrongyliasis with eosinophilic meningitis were tested. Sera from patients with other parasitic illnesses and from healthy volunteers were also analyzed. The results indicate that the immunoblotting used to detect IgG4 antibodies to the antigenic band of an approximate molecular mass of 29 kDa from young adult somatic extract of A. cantonensis is the most reliable test. It gives accuracy, sensitivity, specificity, and positive and negative predictive values of 89.2%, 75%, 95%, 85.7% and 90.4%, respectively. More importantly, the test can discriminate between human angiostrongyliasis, gnathostomiasis and cysticercosis, three diseases that produce eosinophilic meningitis.

Authors+Show Affiliations

Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand. pewpan@kku.ac.thNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12658452

Citation

Intapan, Pewpan M., et al. "Evaluation of Human IgG Subclass Antibodies in the Serodiagnosis of Angiostrongyliasis." Parasitology Research, vol. 89, no. 6, 2003, pp. 425-9.
Intapan PM, Maleewong W, Sawanyawisuth K, et al. Evaluation of human IgG subclass antibodies in the serodiagnosis of angiostrongyliasis. Parasitol Res. 2003;89(6):425-9.
Intapan, P. M., Maleewong, W., Sawanyawisuth, K., & Chotmongkol, V. (2003). Evaluation of human IgG subclass antibodies in the serodiagnosis of angiostrongyliasis. Parasitology Research, 89(6), 425-9.
Intapan PM, et al. Evaluation of Human IgG Subclass Antibodies in the Serodiagnosis of Angiostrongyliasis. Parasitol Res. 2003;89(6):425-9. PubMed PMID: 12658452.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of human IgG subclass antibodies in the serodiagnosis of angiostrongyliasis. AU - Intapan,Pewpan M, AU - Maleewong,Wanchai, AU - Sawanyawisuth,Kittisak, AU - Chotmongkol,Verajit, Y1 - 2002/11/26/ PY - 2002/07/30/received PY - 2002/10/15/accepted PY - 2003/3/27/pubmed PY - 2003/6/24/medline PY - 2003/3/27/entrez SP - 425 EP - 9 JF - Parasitology research JO - Parasitol Res VL - 89 IS - 6 N2 - Immunoglobulin G subclass antibody (IgG1, IgG2, IgG3, and IgG4) responses to the rat lungworm, Angiostrongylus cantonensis, were analyzed using the immunoblotting technique in an attempt to further improve the sensitivity and specificity for the serodiagnosis of human angiostrongyliasis. Serum samples from patients with proven angiostrongyliasis and from clinically suspected cases of angiostrongyliasis with eosinophilic meningitis were tested. Sera from patients with other parasitic illnesses and from healthy volunteers were also analyzed. The results indicate that the immunoblotting used to detect IgG4 antibodies to the antigenic band of an approximate molecular mass of 29 kDa from young adult somatic extract of A. cantonensis is the most reliable test. It gives accuracy, sensitivity, specificity, and positive and negative predictive values of 89.2%, 75%, 95%, 85.7% and 90.4%, respectively. More importantly, the test can discriminate between human angiostrongyliasis, gnathostomiasis and cysticercosis, three diseases that produce eosinophilic meningitis. SN - 0932-0113 UR - https://www.unboundmedicine.com/medline/citation/12658452/Evaluation_of_human_IgG_subclass_antibodies_in_the_serodiagnosis_of_angiostrongyliasis_ L2 - https://dx.doi.org/10.1007/s00436-002-0781-6 DB - PRIME DP - Unbound Medicine ER -