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Analysis of lignans using micellar electrokinetic chromatography.
Electrophoresis. 2003 Mar; 24(6):1047-53.E

Abstract

Micellar electrokinetic chromatography (MEKC) was used to separate twelve lignan compounds originating from Phyllanthus plants. To increase the reliability of peak identification, two micellar systems, the sodium dodecyl sulfate (SDS) and sodium deoxycholate (SDC) systems, were investigated. Because of the high lipophilicity of the lignan analytes, tetrahydrofuran was added to the SDS micellar system to increase its separating ability. In contrast to SDS system, no organic solvent was needed with SDC micelles. Both micellar systems gave a satisfactory separation within a reasonable analysis time. On considering accuracy for quantitation, the SDS method was validated and then used to determine the content of the lignans in two Phyllanthus plants. The selectivity (elution order of the lignans) was significantly different between the SDS and SDC micellar systems. Retention in SDC-MEKC seemed to be dominated by the hydrophobicity of the lignan solutes, while in SDS-MEKC, retention was greatly influenced by hydrogen bonding interactions.

Authors+Show Affiliations

Kuo CH
School of Pharmacy, College of Medicine, National Taiwan University, Taipei, Taiwan.
Lee SS
No affiliation info available
Chang HY
No affiliation info available
Sun SW
No affiliation info available

MeSH

Bile Acids and SaltsChromatography, High Pressure LiquidChromatography, Micellar Electrokinetic CapillaryLignansPhyllanthusReproducibility of ResultsSodium Dodecyl Sulfate

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12658694

Citation

Kuo, Ching-Hua, et al. "Analysis of Lignans Using Micellar Electrokinetic Chromatography." Electrophoresis, vol. 24, no. 6, 2003, pp. 1047-53.
Kuo CH, Lee SS, Chang HY, et al. Analysis of lignans using micellar electrokinetic chromatography. Electrophoresis. 2003;24(6):1047-53.
Kuo, C. H., Lee, S. S., Chang, H. Y., & Sun, S. W. (2003). Analysis of lignans using micellar electrokinetic chromatography. Electrophoresis, 24(6), 1047-53.
Kuo CH, et al. Analysis of Lignans Using Micellar Electrokinetic Chromatography. Electrophoresis. 2003;24(6):1047-53. PubMed PMID: 12658694.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of lignans using micellar electrokinetic chromatography. AU - Kuo,Ching-Hua, AU - Lee,Shoei-Sheng, AU - Chang,Hsing-Yun, AU - Sun,Shao-Wen, PY - 2003/3/27/pubmed PY - 2003/12/3/medline PY - 2003/3/27/entrez SP - 1047 EP - 53 JF - Electrophoresis JO - Electrophoresis VL - 24 IS - 6 N2 - Micellar electrokinetic chromatography (MEKC) was used to separate twelve lignan compounds originating from Phyllanthus plants. To increase the reliability of peak identification, two micellar systems, the sodium dodecyl sulfate (SDS) and sodium deoxycholate (SDC) systems, were investigated. Because of the high lipophilicity of the lignan analytes, tetrahydrofuran was added to the SDS micellar system to increase its separating ability. In contrast to SDS system, no organic solvent was needed with SDC micelles. Both micellar systems gave a satisfactory separation within a reasonable analysis time. On considering accuracy for quantitation, the SDS method was validated and then used to determine the content of the lignans in two Phyllanthus plants. The selectivity (elution order of the lignans) was significantly different between the SDS and SDC micellar systems. Retention in SDC-MEKC seemed to be dominated by the hydrophobicity of the lignan solutes, while in SDS-MEKC, retention was greatly influenced by hydrogen bonding interactions. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/12658694/Analysis_of_lignans_using_micellar_electrokinetic_chromatography_ DB - PRIME DP - Unbound Medicine ER -