Tags

Type your tag names separated by a space and hit enter

Quantification of vascular endothelial growth factor-C and its receptor-3 messenger RNA with real-time quantitative polymerase chain reaction as a predictor of lymph node metastasis in human colorectal cancer.
Surgery. 2003 Mar; 133(3):300-8.S

Abstract

BACKGROUND

This study was undertaken to determine whether vascular endothelial growth factor-C (VEGF-C) and the expression of its receptor VEGF receptor-3 (VEGFR-3) are correlated with lymph node metastasis in human colorectal cancer and whether their expression levels might be used to predict lymph node metastasis.

METHODS

Fifty-three surgical specimens of colorectal cancer with (n = 24) or without (n = 29) lymph node metastasis were studied. The messenger RNA (mRNA) expression of VEGF-C and VEGFR-3 was quantified with a new method for kinetic quantitative polymerase chain reaction (PCR), real-time quantitative (RTQ) reverse transcriptase-PCR. In addition, their protein expressions were assessed immunohistochemically.

RESULTS

The VEGF-C mRNA level in primary tumors correlated with VEGF-C protein expression, lymph node metastasis, and lymphatic invasion. Sixteen of 24 patients with lymph node metastasis showed VEGF-C mRNA overexpression. Morphologically, VEGF-C protein expression in primary tumors showed a statistically significant relationship with lymph node metastasis and lymphatic invasion.

CONCLUSION

Real-time quantitative reverse transcriptase-PCR is a sensitive method for detection and quantification of VEGF-C and VEGFR-3 mRNA expression. Furthermore, the expression levels of VEGF-C mRNA and protein in colorectal cancer are correlated with lymph node metastasis and lymphatic invasion.

Authors+Show Affiliations

First Department of Surgery, Sapporo Medical University, Sapporo, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

12660643

Citation

Kawakami, Masayo, et al. "Quantification of Vascular Endothelial Growth factor-C and Its Receptor-3 Messenger RNA With Real-time Quantitative Polymerase Chain Reaction as a Predictor of Lymph Node Metastasis in Human Colorectal Cancer." Surgery, vol. 133, no. 3, 2003, pp. 300-8.
Kawakami M, Furuhata T, Kimura Y, et al. Quantification of vascular endothelial growth factor-C and its receptor-3 messenger RNA with real-time quantitative polymerase chain reaction as a predictor of lymph node metastasis in human colorectal cancer. Surgery. 2003;133(3):300-8.
Kawakami, M., Furuhata, T., Kimura, Y., Yamaguchi, K., Hata, F., Sasaki, K., & Hirata, K. (2003). Quantification of vascular endothelial growth factor-C and its receptor-3 messenger RNA with real-time quantitative polymerase chain reaction as a predictor of lymph node metastasis in human colorectal cancer. Surgery, 133(3), 300-8.
Kawakami M, et al. Quantification of Vascular Endothelial Growth factor-C and Its Receptor-3 Messenger RNA With Real-time Quantitative Polymerase Chain Reaction as a Predictor of Lymph Node Metastasis in Human Colorectal Cancer. Surgery. 2003;133(3):300-8. PubMed PMID: 12660643.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quantification of vascular endothelial growth factor-C and its receptor-3 messenger RNA with real-time quantitative polymerase chain reaction as a predictor of lymph node metastasis in human colorectal cancer. AU - Kawakami,Masayo, AU - Furuhata,Tomohisa, AU - Kimura,Yasutoshi, AU - Yamaguchi,Koji, AU - Hata,Fumitake, AU - Sasaki,Kazuaki, AU - Hirata,Koichi, PY - 2003/3/28/pubmed PY - 2003/4/24/medline PY - 2003/3/28/entrez SP - 300 EP - 8 JF - Surgery JO - Surgery VL - 133 IS - 3 N2 - BACKGROUND: This study was undertaken to determine whether vascular endothelial growth factor-C (VEGF-C) and the expression of its receptor VEGF receptor-3 (VEGFR-3) are correlated with lymph node metastasis in human colorectal cancer and whether their expression levels might be used to predict lymph node metastasis. METHODS: Fifty-three surgical specimens of colorectal cancer with (n = 24) or without (n = 29) lymph node metastasis were studied. The messenger RNA (mRNA) expression of VEGF-C and VEGFR-3 was quantified with a new method for kinetic quantitative polymerase chain reaction (PCR), real-time quantitative (RTQ) reverse transcriptase-PCR. In addition, their protein expressions were assessed immunohistochemically. RESULTS: The VEGF-C mRNA level in primary tumors correlated with VEGF-C protein expression, lymph node metastasis, and lymphatic invasion. Sixteen of 24 patients with lymph node metastasis showed VEGF-C mRNA overexpression. Morphologically, VEGF-C protein expression in primary tumors showed a statistically significant relationship with lymph node metastasis and lymphatic invasion. CONCLUSION: Real-time quantitative reverse transcriptase-PCR is a sensitive method for detection and quantification of VEGF-C and VEGFR-3 mRNA expression. Furthermore, the expression levels of VEGF-C mRNA and protein in colorectal cancer are correlated with lymph node metastasis and lymphatic invasion. SN - 0039-6060 UR - https://www.unboundmedicine.com/medline/citation/12660643/Quantification_of_vascular_endothelial_growth_factor_C_and_its_receptor_3_messenger_RNA_with_real_time_quantitative_polymerase_chain_reaction_as_a_predictor_of_lymph_node_metastasis_in_human_colorectal_cancer_ DB - PRIME DP - Unbound Medicine ER -