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Glucocorticoid-regulated adipose tissue secretion of PAI-1, but not IL-6, TNFalpha or leptin in vivo.
Horm Metab Res 2002 Nov-Dec; 34(11-12):698-702HM

Abstract

OBJECTIVE

Glucocorticoids are well-known regulators of adipose tissue metabolism and endocrine function. The aim of this study was to examine glucocorticoid effect on plasminogen activator inhibitor 1 (PAI-1), tumour necrosis factor alpha (TNFalpha), leptin and interleukin-6 (IL-6) adipose tissue secretion.

MATERIAL AND METHODS

Twelve healthy postmenopausal women with mean BMI of 28.9 kg/m(2) (+/- 0.8 SEM) received 25 mg prednisolone daily for 7 days. Before and after glucocorticoid treatment adipose tissue secretion of PAI-1, leptin, IL-6 and TNFalpha were measured, and adipocyte PAI-1 mRNA as well as anthropometrical and bio-chemical data were obtained.

RESULTS

Anthropometric measurements remained unaffected. Analyses of venous blood-samples showed a borderline increase of insulin levels (p = 0.062). PAI-1 secretion from adipose tissue increased (1.9 +/- 0.2 vs. 3.5 +/- 0.5 ng/g triglycerides, p = 0.012), but PAI-1 mRNA levels did not (0.19 +/- 0.02 vs. 0.21 +/- 0.04 arbitrary units after normalised to beta-actin, p = 0.51). There were no apparent differences in IL-6, TNFalpha or leptin secretion after glucocorticoid exposure.

CONCLUSION

This study shows an increased secretion of PAI-1, but not IL-6, TNFalpha or leptin, from abdominal adipose tissue after in vivo glucocorticoid treatment, which may be a finding of pathophysiological importance given the well-known effect of glucocorticoid excess on metabolic aberrations and cardiovascular morbidity.

Authors+Show Affiliations

Centre for metabolism and Endocrinology, Huddinge University Hospital, Stochholm, Sweden.No affiliation info availableNo affiliation info available

Pub Type(s)

Clinical Trial
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12660885

Citation

Uddén, J, et al. "Glucocorticoid-regulated Adipose Tissue Secretion of PAI-1, but Not IL-6, TNFalpha or Leptin in Vivo." Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Metabolisme, vol. 34, no. 11-12, 2002, pp. 698-702.
Uddén J, Eriksson P, Hoffstedt J. Glucocorticoid-regulated adipose tissue secretion of PAI-1, but not IL-6, TNFalpha or leptin in vivo. Horm Metab Res. 2002;34(11-12):698-702.
Uddén, J., Eriksson, P., & Hoffstedt, J. (2002). Glucocorticoid-regulated adipose tissue secretion of PAI-1, but not IL-6, TNFalpha or leptin in vivo. Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Metabolisme, 34(11-12), pp. 698-702.
Uddén J, Eriksson P, Hoffstedt J. Glucocorticoid-regulated Adipose Tissue Secretion of PAI-1, but Not IL-6, TNFalpha or Leptin in Vivo. Horm Metab Res. 2002;34(11-12):698-702. PubMed PMID: 12660885.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Glucocorticoid-regulated adipose tissue secretion of PAI-1, but not IL-6, TNFalpha or leptin in vivo. AU - Uddén,J, AU - Eriksson,P, AU - Hoffstedt,J, PY - 2003/3/28/pubmed PY - 2003/10/30/medline PY - 2003/3/28/entrez SP - 698 EP - 702 JF - Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme JO - Horm. Metab. Res. VL - 34 IS - 11-12 N2 - OBJECTIVE: Glucocorticoids are well-known regulators of adipose tissue metabolism and endocrine function. The aim of this study was to examine glucocorticoid effect on plasminogen activator inhibitor 1 (PAI-1), tumour necrosis factor alpha (TNFalpha), leptin and interleukin-6 (IL-6) adipose tissue secretion. MATERIAL AND METHODS: Twelve healthy postmenopausal women with mean BMI of 28.9 kg/m(2) (+/- 0.8 SEM) received 25 mg prednisolone daily for 7 days. Before and after glucocorticoid treatment adipose tissue secretion of PAI-1, leptin, IL-6 and TNFalpha were measured, and adipocyte PAI-1 mRNA as well as anthropometrical and bio-chemical data were obtained. RESULTS: Anthropometric measurements remained unaffected. Analyses of venous blood-samples showed a borderline increase of insulin levels (p = 0.062). PAI-1 secretion from adipose tissue increased (1.9 +/- 0.2 vs. 3.5 +/- 0.5 ng/g triglycerides, p = 0.012), but PAI-1 mRNA levels did not (0.19 +/- 0.02 vs. 0.21 +/- 0.04 arbitrary units after normalised to beta-actin, p = 0.51). There were no apparent differences in IL-6, TNFalpha or leptin secretion after glucocorticoid exposure. CONCLUSION: This study shows an increased secretion of PAI-1, but not IL-6, TNFalpha or leptin, from abdominal adipose tissue after in vivo glucocorticoid treatment, which may be a finding of pathophysiological importance given the well-known effect of glucocorticoid excess on metabolic aberrations and cardiovascular morbidity. SN - 0018-5043 UR - https://www.unboundmedicine.com/medline/citation/12660885/Glucocorticoid_regulated_adipose_tissue_secretion_of_PAI_1_but_not_IL_6_TNFalpha_or_leptin_in_vivo_ L2 - http://www.thieme-connect.com/DOI/DOI?10.1055/s-2002-38257 DB - PRIME DP - Unbound Medicine ER -