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Evaluation of a digestion assay and determination of sample size and tissue for the reliable detection of Trichinella larvae in walrus meat.
J Vet Diagn Invest. 2003 Mar; 15(2):188-91.JV

Abstract

A digestion assay was validated for the detection of Trichinella larvae in walrus (Odobenus rosmarus) meat, and appropriate samples for testing were determined using tissues from infected walruses harvested for food. Examination of muscles from 3 walruses showed that the tongue consistently contained approximately 2-6 times more larvae than the pectoral and intercostal muscles. Comparison of numbers of larvae in the root, body, and apex of the tongue from 3 walruses failed to identify a predilection site within the tongue, but the apex was considered an optimal tissue because of the high larval density within the tongue and the ease of collection. All 31 spiked samples weighing 50 g each and containing between 0.1 and 0.4 larvae per gram (lpg) were correctly identified as infected, indicating that the sensitivity of this procedure is adequate for diagnostic use. A sample size of 10 g consistently detected larvae in 2 walrus tongues containing > or = 0.3 lpg (n = 40), and until additional data are available, sample sizes from individual walrus tongues should be a minimum of 10 g. This study provides the preliminary data that were used for the development of a food safety analytical protocol for the detection of Trichinella in walrus meat in arctic communities.

Authors+Show Affiliations

Nunavik Research Centre, Makivik Corporation, PO Box 179, Kuujjuaq, Quebec J0M 1C0, Canada.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12661733

Citation

Leclair, Daniel, et al. "Evaluation of a Digestion Assay and Determination of Sample Size and Tissue for the Reliable Detection of Trichinella Larvae in Walrus Meat." Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc, vol. 15, no. 2, 2003, pp. 188-91.
Leclair D, Forbes LB, Suppa S, et al. Evaluation of a digestion assay and determination of sample size and tissue for the reliable detection of Trichinella larvae in walrus meat. J Vet Diagn Invest. 2003;15(2):188-91.
Leclair, D., Forbes, L. B., Suppa, S., & Gajadhar, A. A. (2003). Evaluation of a digestion assay and determination of sample size and tissue for the reliable detection of Trichinella larvae in walrus meat. Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc, 15(2), 188-91.
Leclair D, et al. Evaluation of a Digestion Assay and Determination of Sample Size and Tissue for the Reliable Detection of Trichinella Larvae in Walrus Meat. J Vet Diagn Invest. 2003;15(2):188-91. PubMed PMID: 12661733.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of a digestion assay and determination of sample size and tissue for the reliable detection of Trichinella larvae in walrus meat. AU - Leclair,Daniel, AU - Forbes,Lorry B, AU - Suppa,Sandy, AU - Gajadhar,Alvin A, PY - 2003/3/29/pubmed PY - 2003/5/29/medline PY - 2003/3/29/entrez SP - 188 EP - 91 JF - Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc JO - J. Vet. Diagn. Invest. VL - 15 IS - 2 N2 - A digestion assay was validated for the detection of Trichinella larvae in walrus (Odobenus rosmarus) meat, and appropriate samples for testing were determined using tissues from infected walruses harvested for food. Examination of muscles from 3 walruses showed that the tongue consistently contained approximately 2-6 times more larvae than the pectoral and intercostal muscles. Comparison of numbers of larvae in the root, body, and apex of the tongue from 3 walruses failed to identify a predilection site within the tongue, but the apex was considered an optimal tissue because of the high larval density within the tongue and the ease of collection. All 31 spiked samples weighing 50 g each and containing between 0.1 and 0.4 larvae per gram (lpg) were correctly identified as infected, indicating that the sensitivity of this procedure is adequate for diagnostic use. A sample size of 10 g consistently detected larvae in 2 walrus tongues containing > or = 0.3 lpg (n = 40), and until additional data are available, sample sizes from individual walrus tongues should be a minimum of 10 g. This study provides the preliminary data that were used for the development of a food safety analytical protocol for the detection of Trichinella in walrus meat in arctic communities. SN - 1040-6387 UR - https://www.unboundmedicine.com/medline/citation/12661733/Evaluation_of_a_digestion_assay_and_determination_of_sample_size_and_tissue_for_the_reliable_detection_of_Trichinella_larvae_in_walrus_meat_ L2 - http://journals.sagepub.com/doi/full/10.1177/104063870301500217?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -