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Effects of dietary vitamin E and selenium on antioxidative defense mechanisms in the liver of rats treated with high doses of glucocorticoid.
Biol Trace Elem Res. 2003 Mar; 91(3):231-41.BT

Abstract

The aim of this work was to determine the effects of dietary intake vitamin E and selenium (Se) on lipid peroxidation as thiobarbituric acid reactive substances (TBARS) and on the antioxidative defense mechanisms in the liver of rats treated with high doses of prednisolone. Two hundred fifty adult male Wistar rats were randomly divided into five groups. The rats were fed a normal diet, but groups 3, 4, and 5 received a daily supplement in their drinking water of 20 mg vitamin E, 0.3 mg Se, and a combination of vitamin E and Se, respectively, for 30 d. For 3 d subsequently, the control group (group 1) was treated with a placebo, and the remaining four groups were injected intramuscularly with 100 mg/kg body weight (BW) prednisolone. After the last administration of prednisolone, 10 rats from each group were killed at 4, 8, 12, 24, and 48 h and the activities of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and catalase (CAT) enzymes and the levels of glutathione (GSH) and TBARS in their livers were measured. GSH-Px, SOD, and CAT enzyme activities and GSH levels in prednisolone-treatment group (group 2) began to decrease gradually at 4 h, falling respectively to 38%, 55%, and 40% of the control levels by 24 h, and recovering to the control levels at 48 h. In contrast, prednisolone administration caused an increase in the hepatic TBARS, reaching up to four times the levels of the control at 24 h. However, supplementation with vitamin E and Se had a preventive effect on the elevation of the hepatic TBARS and improved the diminished activities of the antioxidative enzymes and the levels of GSH. Therefore, the present study demonstrates the effectiveness of vitamin E and Se in reducing hepatic damage in glucocorticoid- treated rats and suggests that reductions in increased TBARS as a result of prednisolone may be an important factor in the action of vitamin E and Se.

Authors+Show Affiliations

Department of Physiology, Veterinary Faculty of Kafkas University, Kars, Turkey.No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

12663947

Citation

Beytut, Ebru, and Mesut Aksakal. "Effects of Dietary Vitamin E and Selenium On Antioxidative Defense Mechanisms in the Liver of Rats Treated With High Doses of Glucocorticoid." Biological Trace Element Research, vol. 91, no. 3, 2003, pp. 231-41.
Beytut E, Aksakal M. Effects of dietary vitamin E and selenium on antioxidative defense mechanisms in the liver of rats treated with high doses of glucocorticoid. Biol Trace Elem Res. 2003;91(3):231-41.
Beytut, E., & Aksakal, M. (2003). Effects of dietary vitamin E and selenium on antioxidative defense mechanisms in the liver of rats treated with high doses of glucocorticoid. Biological Trace Element Research, 91(3), 231-41.
Beytut E, Aksakal M. Effects of Dietary Vitamin E and Selenium On Antioxidative Defense Mechanisms in the Liver of Rats Treated With High Doses of Glucocorticoid. Biol Trace Elem Res. 2003;91(3):231-41. PubMed PMID: 12663947.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of dietary vitamin E and selenium on antioxidative defense mechanisms in the liver of rats treated with high doses of glucocorticoid. AU - Beytut,Ebru, AU - Aksakal,Mesut, PY - 2002/01/30/received PY - 2002/04/30/revised PY - 2002/06/06/accepted PY - 2003/3/29/pubmed PY - 2003/12/3/medline PY - 2003/3/29/entrez SP - 231 EP - 41 JF - Biological trace element research JO - Biol Trace Elem Res VL - 91 IS - 3 N2 - The aim of this work was to determine the effects of dietary intake vitamin E and selenium (Se) on lipid peroxidation as thiobarbituric acid reactive substances (TBARS) and on the antioxidative defense mechanisms in the liver of rats treated with high doses of prednisolone. Two hundred fifty adult male Wistar rats were randomly divided into five groups. The rats were fed a normal diet, but groups 3, 4, and 5 received a daily supplement in their drinking water of 20 mg vitamin E, 0.3 mg Se, and a combination of vitamin E and Se, respectively, for 30 d. For 3 d subsequently, the control group (group 1) was treated with a placebo, and the remaining four groups were injected intramuscularly with 100 mg/kg body weight (BW) prednisolone. After the last administration of prednisolone, 10 rats from each group were killed at 4, 8, 12, 24, and 48 h and the activities of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and catalase (CAT) enzymes and the levels of glutathione (GSH) and TBARS in their livers were measured. GSH-Px, SOD, and CAT enzyme activities and GSH levels in prednisolone-treatment group (group 2) began to decrease gradually at 4 h, falling respectively to 38%, 55%, and 40% of the control levels by 24 h, and recovering to the control levels at 48 h. In contrast, prednisolone administration caused an increase in the hepatic TBARS, reaching up to four times the levels of the control at 24 h. However, supplementation with vitamin E and Se had a preventive effect on the elevation of the hepatic TBARS and improved the diminished activities of the antioxidative enzymes and the levels of GSH. Therefore, the present study demonstrates the effectiveness of vitamin E and Se in reducing hepatic damage in glucocorticoid- treated rats and suggests that reductions in increased TBARS as a result of prednisolone may be an important factor in the action of vitamin E and Se. SN - 0163-4984 UR - https://www.unboundmedicine.com/medline/citation/12663947/Effects_of_dietary_vitamin_E_and_selenium_on_antioxidative_defense_mechanisms_in_the_liver_of_rats_treated_with_high_doses_of_glucocorticoid_ L2 - https://dx.doi.org/10.1385/BTER:91:3:231 DB - PRIME DP - Unbound Medicine ER -