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[Relationship between cytochrome c-mediated caspase-3 activity and chemoresistance in cisplatin-resistant human ovarian cancer cell lines].
Zhonghua Zhong Liu Za Zhi. 2002 Nov; 24(6):544-7.ZZ

Abstract

OBJECTIVE

To investigate the effects of anti-apoptosis gene (bcl-X(L)), cytochrome c and caspase-3 activity on chemoresistance in cisplatin-resistant human ovarian cancer cell lines (A2780/DDP, COC1/DDP).

METHODS

The expression of bcl-X(L) cisplatin treated cytochrome c and caspase-3 activity were monitored by RT-PCR and Western blot in cisplatin-resistant (A2780/DDP, COC1/DDP) and cisplatin-sensitive (A2780, COC1) cell lines. The apoptotic rates of A2780, COC1, A2780/DDP and COC1/DDP were detected with flow cytometry after having been treated by cisplatin.

RESULTS

The expression of bcl-X(L) in A2780/DDP and COC1/DDP was significantly higher than that in A2780 and COC1 cells, whereas the expression of cytochrome c, caspase-3 activity and apoptotic rates of A2780/DDP and COC1/DDP were significantly reduced more than those of A2780 and COC1 after having been treated by cisplatin (P < 0.05).

CONCLUSION

The overexpression of anti-apoptotic gene bcl-X(L), which downregulates cytochrome c and decreases caspase-3 activity, may be related to cisplatin-resistance in human ovarian cancer cell lines.

Authors+Show Affiliations

Department of Obstetrics & Gynecology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

12667321

Citation

Yang, Xiaokui, et al. "[Relationship Between Cytochrome C-mediated Caspase-3 Activity and Chemoresistance in Cisplatin-resistant Human Ovarian Cancer Cell Lines]." Zhonghua Zhong Liu Za Zhi [Chinese Journal of Oncology], vol. 24, no. 6, 2002, pp. 544-7.
Yang X, Zheng F, Xing H, et al. [Relationship between cytochrome c-mediated caspase-3 activity and chemoresistance in cisplatin-resistant human ovarian cancer cell lines]. Zhonghua Zhong Liu Za Zhi. 2002;24(6):544-7.
Yang, X., Zheng, F., Xing, H., Gao, Q., Wang, W., Lu, Y., Wang, S., & Ma, D. (2002). [Relationship between cytochrome c-mediated caspase-3 activity and chemoresistance in cisplatin-resistant human ovarian cancer cell lines]. Zhonghua Zhong Liu Za Zhi [Chinese Journal of Oncology], 24(6), 544-7.
Yang X, et al. [Relationship Between Cytochrome C-mediated Caspase-3 Activity and Chemoresistance in Cisplatin-resistant Human Ovarian Cancer Cell Lines]. Zhonghua Zhong Liu Za Zhi. 2002;24(6):544-7. PubMed PMID: 12667321.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Relationship between cytochrome c-mediated caspase-3 activity and chemoresistance in cisplatin-resistant human ovarian cancer cell lines]. AU - Yang,Xiaokui, AU - Zheng,Fang, AU - Xing,Hui, AU - Gao,Qinglei, AU - Wang,Wei, AU - Lu,Yunping, AU - Wang,Shixuan, AU - Ma,Ding, PY - 2003/4/2/pubmed PY - 2003/6/24/medline PY - 2003/4/2/entrez SP - 544 EP - 7 JF - Zhonghua zhong liu za zhi [Chinese journal of oncology] JO - Zhonghua Zhong Liu Za Zhi VL - 24 IS - 6 N2 - OBJECTIVE: To investigate the effects of anti-apoptosis gene (bcl-X(L)), cytochrome c and caspase-3 activity on chemoresistance in cisplatin-resistant human ovarian cancer cell lines (A2780/DDP, COC1/DDP). METHODS: The expression of bcl-X(L) cisplatin treated cytochrome c and caspase-3 activity were monitored by RT-PCR and Western blot in cisplatin-resistant (A2780/DDP, COC1/DDP) and cisplatin-sensitive (A2780, COC1) cell lines. The apoptotic rates of A2780, COC1, A2780/DDP and COC1/DDP were detected with flow cytometry after having been treated by cisplatin. RESULTS: The expression of bcl-X(L) in A2780/DDP and COC1/DDP was significantly higher than that in A2780 and COC1 cells, whereas the expression of cytochrome c, caspase-3 activity and apoptotic rates of A2780/DDP and COC1/DDP were significantly reduced more than those of A2780 and COC1 after having been treated by cisplatin (P < 0.05). CONCLUSION: The overexpression of anti-apoptotic gene bcl-X(L), which downregulates cytochrome c and decreases caspase-3 activity, may be related to cisplatin-resistance in human ovarian cancer cell lines. SN - 0253-3766 UR - https://www.unboundmedicine.com/medline/citation/12667321/[Relationship_between_cytochrome_c_mediated_caspase_3_activity_and_chemoresistance_in_cisplatin_resistant_human_ovarian_cancer_cell_lines]_ L2 - http://www.diseaseinfosearch.org/result/5504 DB - PRIME DP - Unbound Medicine ER -