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Fruit juice consumption modulates antioxidative status, immune status and DNA damage.

Abstract

Polyphenolic compounds exert a variety of physiological effects in vitro including antioxidative, immunomodulatory and antigenotoxic effects. In a randomized crossover study in healthy men on a low-polyphenol diet, we determined the effects of 2 polyphenol-rich juices (330 ml/d) supplemented for 2 weeks on bioavailability of polyphenols, markers of antioxidative and immune status, and reduction of DNA damage. Juices provided 236 mg (A) and 226 mg (B) polyphenols with cyanidin glycosides (A) and epigallocatechin gallate (B) as major polyphenolic ingredients. There was no accumulation of plasma polyphenols after two weeks of juice supplementation. In contrast, plasma malondialdehyde decreased with time during juice interventions. Moreover, juice consumption also increased lymphocyte proliferative responsiveness, with no difference between the two juices. Interleukin-2 secretion by activated lymphocytes and the lytic activity of natural killer cells were significantly increased by both juices. Juice intervention had no effect on single DNA strand breaks, but significantly reduced oxidative DNA damage in lymphocytes. A time-delay was observed between the intake of fruit juice and the reduction of oxidative DNA damage and the increase in interleukin-2 secretion. We conclude that consumption of either juice enhanced antioxidant status, reduced oxidative DNA damage and stimulated immune cell functions. However, fruit juice consumption for 2 weeks did not result in elevated plasma polyphenols in subjects after overnight fasting. Further studies should focus on the time-delay between juice intake and changes in measured physiological functions, as well as on active polyphenolic metabolites mediating the observed effects.

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  • Authors+Show Affiliations

    ,

    Institute of Nutritional Physiology, Federal Research Centre for Nutrition, Haid-und-Neu-Str. 9, 76131 Karlsruhe, Germany. achim.bub@bfe.uni-karlsuhe.de

    , , , , , ,

    Source

    MeSH

    Adult
    Antioxidants
    Beverages
    Biological Availability
    Biomarkers
    Carotenoids
    Cross-Over Studies
    DNA Damage
    Flavonoids
    Fruit
    Humans
    Immunity
    Interleukin-2
    Lymphocyte Activation
    Male
    Malondialdehyde
    Oxidation-Reduction
    Phenols
    Polymers
    Polyphenols

    Pub Type(s)

    Clinical Trial
    Journal Article
    Randomized Controlled Trial
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    12667600

    Citation

    Bub, Achim, et al. "Fruit Juice Consumption Modulates Antioxidative Status, Immune Status and DNA Damage." The Journal of Nutritional Biochemistry, vol. 14, no. 2, 2003, pp. 90-8.
    Bub A, Watzl B, Blockhaus M, et al. Fruit juice consumption modulates antioxidative status, immune status and DNA damage. J Nutr Biochem. 2003;14(2):90-8.
    Bub, A., Watzl, B., Blockhaus, M., Briviba, K., Liegibel, U., Müller, H., ... Rechkemmer, G. (2003). Fruit juice consumption modulates antioxidative status, immune status and DNA damage. The Journal of Nutritional Biochemistry, 14(2), pp. 90-8.
    Bub A, et al. Fruit Juice Consumption Modulates Antioxidative Status, Immune Status and DNA Damage. J Nutr Biochem. 2003;14(2):90-8. PubMed PMID: 12667600.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Fruit juice consumption modulates antioxidative status, immune status and DNA damage. AU - Bub,Achim, AU - Watzl,Bernhard, AU - Blockhaus,Mark, AU - Briviba,Karlis, AU - Liegibel,Ute, AU - Müller,Harald, AU - Pool-Zobel,Beatrice L, AU - Rechkemmer,Gerhard, PY - 2003/4/2/pubmed PY - 2003/10/4/medline PY - 2003/4/2/entrez SP - 90 EP - 8 JF - The Journal of nutritional biochemistry JO - J. Nutr. Biochem. VL - 14 IS - 2 N2 - Polyphenolic compounds exert a variety of physiological effects in vitro including antioxidative, immunomodulatory and antigenotoxic effects. In a randomized crossover study in healthy men on a low-polyphenol diet, we determined the effects of 2 polyphenol-rich juices (330 ml/d) supplemented for 2 weeks on bioavailability of polyphenols, markers of antioxidative and immune status, and reduction of DNA damage. Juices provided 236 mg (A) and 226 mg (B) polyphenols with cyanidin glycosides (A) and epigallocatechin gallate (B) as major polyphenolic ingredients. There was no accumulation of plasma polyphenols after two weeks of juice supplementation. In contrast, plasma malondialdehyde decreased with time during juice interventions. Moreover, juice consumption also increased lymphocyte proliferative responsiveness, with no difference between the two juices. Interleukin-2 secretion by activated lymphocytes and the lytic activity of natural killer cells were significantly increased by both juices. Juice intervention had no effect on single DNA strand breaks, but significantly reduced oxidative DNA damage in lymphocytes. A time-delay was observed between the intake of fruit juice and the reduction of oxidative DNA damage and the increase in interleukin-2 secretion. We conclude that consumption of either juice enhanced antioxidant status, reduced oxidative DNA damage and stimulated immune cell functions. However, fruit juice consumption for 2 weeks did not result in elevated plasma polyphenols in subjects after overnight fasting. Further studies should focus on the time-delay between juice intake and changes in measured physiological functions, as well as on active polyphenolic metabolites mediating the observed effects. SN - 0955-2863 UR - https://www.unboundmedicine.com/medline/citation/12667600/Fruit_juice_consumption_modulates_antioxidative_status_immune_status_and_DNA_damage_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0955286302002553 DB - PRIME DP - Unbound Medicine ER -