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Development of a rapid and sensitive method for the quantitation of amphetamines in human plasma and oral fluid by LC-MS-MS.
J Anal Toxicol. 2003 Mar; 27(2):78-87.JA

Abstract

Target analysis of amphetamines in biological samples is of great importance for clinical and forensic toxicologists alike. At present, most laboratories analyze such samples by gas chromatography-mass spectrometry. However, this procedure is labor-intensive and time-consuming, particularly as a preliminary extraction and derivatization are usually unavoidable. Here we describe the development of an alternative method. Amphetamines were isolated from human plasma and oral fluid using a simple methanol precipitation step and subsequently analyzed using reversed-phase liquid chromatography-tandem mass spectrometry. Quantitation of the drugs was performed using multiple reaction monitoring. The developed method, which requires only 50 microL of biological sample, has a total analysis time of less than 20 min (including sample preparation) and enables the simultaneous quantitation of 3,4-methylenedioxymethamphetamine, 3,4-methylenedioxyamphetamine, 3,4-methylenedioxyethylamphetamine, amphetamine, methamphetamine, and ephedrine in a single chromatographic run. Limits of detection of 2 microg/L or better were obtained. The method has been validated and subsequently applied to the analysis of plasma and oral fluid samples collected from current drug users.

Authors+Show Affiliations

Micromass U.K. Limited, Atlas Park, Simonsway, Wythenshawe, Manchester M22 5PP, United Kingdom. michelle.wood@micromass.co.ukNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

12670001

Citation

Wood, M, et al. "Development of a Rapid and Sensitive Method for the Quantitation of Amphetamines in Human Plasma and Oral Fluid By LC-MS-MS." Journal of Analytical Toxicology, vol. 27, no. 2, 2003, pp. 78-87.
Wood M, De Boeck G, Samyn N, et al. Development of a rapid and sensitive method for the quantitation of amphetamines in human plasma and oral fluid by LC-MS-MS. J Anal Toxicol. 2003;27(2):78-87.
Wood, M., De Boeck, G., Samyn, N., Morris, M., Cooper, D. P., Maes, R. A., & De Bruijn, E. A. (2003). Development of a rapid and sensitive method for the quantitation of amphetamines in human plasma and oral fluid by LC-MS-MS. Journal of Analytical Toxicology, 27(2), 78-87.
Wood M, et al. Development of a Rapid and Sensitive Method for the Quantitation of Amphetamines in Human Plasma and Oral Fluid By LC-MS-MS. J Anal Toxicol. 2003;27(2):78-87. PubMed PMID: 12670001.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development of a rapid and sensitive method for the quantitation of amphetamines in human plasma and oral fluid by LC-MS-MS. AU - Wood,M, AU - De Boeck,G, AU - Samyn,N, AU - Morris,M, AU - Cooper,D P, AU - Maes,R A A, AU - De Bruijn,E A, PY - 2003/4/3/pubmed PY - 2003/10/31/medline PY - 2003/4/3/entrez SP - 78 EP - 87 JF - Journal of analytical toxicology JO - J Anal Toxicol VL - 27 IS - 2 N2 - Target analysis of amphetamines in biological samples is of great importance for clinical and forensic toxicologists alike. At present, most laboratories analyze such samples by gas chromatography-mass spectrometry. However, this procedure is labor-intensive and time-consuming, particularly as a preliminary extraction and derivatization are usually unavoidable. Here we describe the development of an alternative method. Amphetamines were isolated from human plasma and oral fluid using a simple methanol precipitation step and subsequently analyzed using reversed-phase liquid chromatography-tandem mass spectrometry. Quantitation of the drugs was performed using multiple reaction monitoring. The developed method, which requires only 50 microL of biological sample, has a total analysis time of less than 20 min (including sample preparation) and enables the simultaneous quantitation of 3,4-methylenedioxymethamphetamine, 3,4-methylenedioxyamphetamine, 3,4-methylenedioxyethylamphetamine, amphetamine, methamphetamine, and ephedrine in a single chromatographic run. Limits of detection of 2 microg/L or better were obtained. The method has been validated and subsequently applied to the analysis of plasma and oral fluid samples collected from current drug users. SN - 0146-4760 UR - https://www.unboundmedicine.com/medline/citation/12670001/Development_of_a_rapid_and_sensitive_method_for_the_quantitation_of_amphetamines_in_human_plasma_and_oral_fluid_by_LC_MS_MS_ L2 - https://academic.oup.com/jat/article-lookup/doi/10.1093/jat/27.2.78 DB - PRIME DP - Unbound Medicine ER -