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[The relationship of methylenetetrahydrofolate reductase gene polymorphism and plasma homocysteine levels in type 2 diabetes mellitus patients with diabetic retinopathy].
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2003 Apr; 20(2):131-4.ZY

Abstract

OBJECTIVE

To evaluate the role of methylenetetrahydrofolate reductase (MTHFR) gene polymorphisms and plasma homocysteine levels in Chinese patients with type 2 diabetes mellitus and diabetic retinopathy (DR).

METHODS

MTHFR genetic C677T polymorphisms were determined by PCR-restriction fragment length polymorphism. Total plasma homocysteine levels were measured using high-performance liquid chromatography (HPLC) with fluorescence detection.

RESULTS

The frequencies of MTHFR T homogenetic type and CT heterogenetic type and allele T (28.18%, 41.82%, 49.09%) in type 2 diabetic patients with diabetic retinopathy were significantly higher than those in diabetic patients without retinopathy (18.37%,29.59%,33.16%) or the normal controls (17.54%, 28.07%, 31.58%). Howerver, there were no significant differences in the frequency of MTHFR genotype and allele between the type 2 diabetic patients without retinopathy and the normal controls. The presence of T allele appeared to have a strong association with the development of diabetic retinopathy. The odds ratio was 1.94 and the 95% confidence interval was 1.31-2.88. Moreover, the plasma homocysteine levels in patients with TT or CT genotype were markedly higher than those in patients with CC genotype.

CONCLUSION

MTHFR gene C677T mutation associated with a predisposition to increase of plasma homocysteine may represent a genetic risk factor for diabetic retinopathy in Chinese type 2 diabetes mellitus.

Authors+Show Affiliations

Sun J
Department of Endocrinology, Zhongnan Hospital, Wuhan University,Wuhan, Hubei, 430071 P. R. China. sjz300@163.com
Xu Y
No affiliation info available
Zhu Y
No affiliation info available
Lu H
No affiliation info available
Deng H
No affiliation info available
Fan Y
No affiliation info available
Sun S
No affiliation info available
Zhang Y
No affiliation info available

MeSH

AdultAllelesDNADeoxyribonucleases, Type II Site-SpecificDiabetes Mellitus, Type 2Diabetic RetinopathyFemaleGene FrequencyGenotypeHomocysteineHumansMaleMethylenetetrahydrofolate Reductase (NADPH2)Middle AgedOxidoreductases Acting on CH-NH Group DonorsPoint MutationPolymorphism, Genetic

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

12673582

Citation

Sun, Jiazhong, et al. "[The Relationship of Methylenetetrahydrofolate Reductase Gene Polymorphism and Plasma Homocysteine Levels in Type 2 Diabetes Mellitus Patients With Diabetic Retinopathy]." Zhonghua Yi Xue Yi Chuan Xue Za Zhi = Zhonghua Yixue Yichuanxue Zazhi = Chinese Journal of Medical Genetics, vol. 20, no. 2, 2003, pp. 131-4.
Sun J, Xu Y, Zhu Y, et al. [The relationship of methylenetetrahydrofolate reductase gene polymorphism and plasma homocysteine levels in type 2 diabetes mellitus patients with diabetic retinopathy]. Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2003;20(2):131-4.
Sun, J., Xu, Y., Zhu, Y., Lu, H., Deng, H., Fan, Y., Sun, S., & Zhang, Y. (2003). [The relationship of methylenetetrahydrofolate reductase gene polymorphism and plasma homocysteine levels in type 2 diabetes mellitus patients with diabetic retinopathy]. Zhonghua Yi Xue Yi Chuan Xue Za Zhi = Zhonghua Yixue Yichuanxue Zazhi = Chinese Journal of Medical Genetics, 20(2), 131-4.
Sun J, et al. [The Relationship of Methylenetetrahydrofolate Reductase Gene Polymorphism and Plasma Homocysteine Levels in Type 2 Diabetes Mellitus Patients With Diabetic Retinopathy]. Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2003;20(2):131-4. PubMed PMID: 12673582.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [The relationship of methylenetetrahydrofolate reductase gene polymorphism and plasma homocysteine levels in type 2 diabetes mellitus patients with diabetic retinopathy]. AU - Sun,Jiazhong, AU - Xu,Yancheng, AU - Zhu,Yilian, AU - Lu,Hongyun, AU - Deng,Haohua, AU - Fan,Youjun, AU - Sun,Suxin, AU - Zhang,Ying, PY - 2003/4/4/pubmed PY - 2003/9/17/medline PY - 2003/4/4/entrez SP - 131 EP - 4 JF - Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics JO - Zhonghua Yi Xue Yi Chuan Xue Za Zhi VL - 20 IS - 2 N2 - OBJECTIVE: To evaluate the role of methylenetetrahydrofolate reductase (MTHFR) gene polymorphisms and plasma homocysteine levels in Chinese patients with type 2 diabetes mellitus and diabetic retinopathy (DR). METHODS: MTHFR genetic C677T polymorphisms were determined by PCR-restriction fragment length polymorphism. Total plasma homocysteine levels were measured using high-performance liquid chromatography (HPLC) with fluorescence detection. RESULTS: The frequencies of MTHFR T homogenetic type and CT heterogenetic type and allele T (28.18%, 41.82%, 49.09%) in type 2 diabetic patients with diabetic retinopathy were significantly higher than those in diabetic patients without retinopathy (18.37%,29.59%,33.16%) or the normal controls (17.54%, 28.07%, 31.58%). Howerver, there were no significant differences in the frequency of MTHFR genotype and allele between the type 2 diabetic patients without retinopathy and the normal controls. The presence of T allele appeared to have a strong association with the development of diabetic retinopathy. The odds ratio was 1.94 and the 95% confidence interval was 1.31-2.88. Moreover, the plasma homocysteine levels in patients with TT or CT genotype were markedly higher than those in patients with CC genotype. CONCLUSION: MTHFR gene C677T mutation associated with a predisposition to increase of plasma homocysteine may represent a genetic risk factor for diabetic retinopathy in Chinese type 2 diabetes mellitus. SN - 1003-9406 UR - https://www.unboundmedicine.com/medline/citation/12673582/[The_relationship_of_methylenetetrahydrofolate_reductase_gene_polymorphism_and_plasma_homocysteine_levels_in_type_2_diabetes_mellitus_patients_with_diabetic_retinopathy]_ DB - PRIME DP - Unbound Medicine ER -