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Characterization and behaviour of alpha-glucan synthase in Schizosaccharomyces pombe as revealed by electron microscopy.
Yeast. 2003 Apr 15; 20(5):427-38.Y

Abstract

Alpha-1,3-Glucan is a cell wall component in Schizosaccharomyces pombe and is exclusive to budding yeast. We analysed the ultrastructure of the cell wall in the alpha-glucan synthase mutant mok1 and determined the role of alpha-1,3-glucan in cell wall formation of Sz. pombe. The mok1 mutant cell has an abnormal shape, with swelling at the tip or at the site of the septum. The cell wall is thicker and looser than that of wild-type cells, and the layered structure of the cell wall is broken. The glucan fibrils forming the protoplast retain a fine fibril structure, although their development into bundles is abnormal. We also report the localization of Mok1p by immunoelectron microscopy using high-pressure freeze substitution and SDS-digested freeze-fracture replica labelling methods. The Mok1p is localized on the cell membrane and moves from the cell tip to the medial region during the cell cycle. These results confirm that Mok1p plays an important role in the normal construction of the cell wall and in the primary step of glucan bundle formation, and that it is required for new cell wall synthesis during vegetative growth. These findings suggest that alpha-1,3-glucan is an essential component for cell wall formation in fission yeast.

Authors+Show Affiliations

Konomi M
Laboratory of Electron Microscopy, Faculty of Science, Japan Women's University, 2-8-1, Mejirodai, Bunkyo-ku, Tokyo 112 -8681, Japan.
Fujimoto K
No affiliation info available
Toda T
No affiliation info available
Osumi M
No affiliation info available

MeSH

Cell WallCytoskeletonFreeze FracturingGlucansGlucosyltransferasesMicroscopy, Electron, ScanningMicroscopy, ImmunoelectronMutationSchizosaccharomycesSchizosaccharomyces pombe Proteins

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12673626

Citation

Konomi, Mami, et al. "Characterization and Behaviour of Alpha-glucan Synthase in Schizosaccharomyces Pombe as Revealed By Electron Microscopy." Yeast (Chichester, England), vol. 20, no. 5, 2003, pp. 427-38.
Konomi M, Fujimoto K, Toda T, et al. Characterization and behaviour of alpha-glucan synthase in Schizosaccharomyces pombe as revealed by electron microscopy. Yeast. 2003;20(5):427-38.
Konomi, M., Fujimoto, K., Toda, T., & Osumi, M. (2003). Characterization and behaviour of alpha-glucan synthase in Schizosaccharomyces pombe as revealed by electron microscopy. Yeast (Chichester, England), 20(5), 427-38.
Konomi M, et al. Characterization and Behaviour of Alpha-glucan Synthase in Schizosaccharomyces Pombe as Revealed By Electron Microscopy. Yeast. 2003 Apr 15;20(5):427-38. PubMed PMID: 12673626.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization and behaviour of alpha-glucan synthase in Schizosaccharomyces pombe as revealed by electron microscopy. AU - Konomi,Mami, AU - Fujimoto,Kazushi, AU - Toda,Takashi, AU - Osumi,Masako, PY - 2003/4/4/pubmed PY - 2003/6/7/medline PY - 2003/4/4/entrez SP - 427 EP - 38 JF - Yeast (Chichester, England) JO - Yeast VL - 20 IS - 5 N2 - Alpha-1,3-Glucan is a cell wall component in Schizosaccharomyces pombe and is exclusive to budding yeast. We analysed the ultrastructure of the cell wall in the alpha-glucan synthase mutant mok1 and determined the role of alpha-1,3-glucan in cell wall formation of Sz. pombe. The mok1 mutant cell has an abnormal shape, with swelling at the tip or at the site of the septum. The cell wall is thicker and looser than that of wild-type cells, and the layered structure of the cell wall is broken. The glucan fibrils forming the protoplast retain a fine fibril structure, although their development into bundles is abnormal. We also report the localization of Mok1p by immunoelectron microscopy using high-pressure freeze substitution and SDS-digested freeze-fracture replica labelling methods. The Mok1p is localized on the cell membrane and moves from the cell tip to the medial region during the cell cycle. These results confirm that Mok1p plays an important role in the normal construction of the cell wall and in the primary step of glucan bundle formation, and that it is required for new cell wall synthesis during vegetative growth. These findings suggest that alpha-1,3-glucan is an essential component for cell wall formation in fission yeast. SN - 0749-503X UR - https://www.unboundmedicine.com/medline/citation/12673626/Characterization_and_behaviour_of_alpha_glucan_synthase_in_Schizosaccharomyces_pombe_as_revealed_by_electron_microscopy_ DB - PRIME DP - Unbound Medicine ER -