Tags

Type your tag names separated by a space and hit enter

Expression of S-100 protein is related to neuronal damage in MPTP-treated mice.

Abstract

S-100beta is a calcium-binding protein expressed at high levels in brain and is known as a marker of brain damage. However, little is known about the role of S-100beta protein during neuronal damage caused by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). To determine whether S-100beta protein is induced in glial cells after MPTP treatment, we investigated the expression of S-100 protein immunohistochemically, using MPTP-treated mice. We also examined the change of neurons and glial cells in mice after MPTP treatment. The present study shows that tyrosine hydroxylase (TH) immunoreactivity decreased gradually in the striatum and substantia nigra from 1 day after MPTP treatment. Thereafter, TH-immunopositive cells and fibers decreased in the striatum and substantia nigra at 3 days after MPTP treatment. In contrast, S-100-immunopositive cells and glial fibrillary acidic protein (GFAP)-immunopositive cells increased markedly in the striatum and substantia nigra at 3 days after MPTP treatment. Seven days after MPTP treatment, S-100-immunopositive cells decreased in the striatum and substantia nigra. However, the number of GFAP-immunopositive cells increased in these regions. In double-labeled immunostaining with anti-S-100 and anti-GFAP antibodies, S-100 immunoreactivity was observed only in the GFAP-positive astrocytes. These results provide evidence that astrocytic activation may play a role in the pathogenesis of MPTP-induced degeneration of dopaminergic neurons. Furthermore, the present study demonstrates that S-100 protein is expressed selectively by astrocytes, but not by microglia, after MPTP treatment. These results provide valuable information for the pathogenesis of the acute stage of Parkinson's disease.

Links

  • Publisher Full Text
  • Authors+Show Affiliations

    ,

    Department of Clinical Pharmacology and Therapeutics, Tohoku University Graduate School of Pharmaceutical Science and Medicine, Sendai, Japan.

    , , , , ,

    Source

    Glia 42:3 2003 May pg 307-13

    MeSH

    1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine
    Animals
    Astrocytes
    Biomarkers
    Dopamine
    Down-Regulation
    Gene Expression
    Glial Fibrillary Acidic Protein
    Gliosis
    Immunohistochemistry
    Mice
    Microglia
    Neostriatum
    Nerve Degeneration
    Nerve Growth Factors
    Neurons
    Parkinsonian Disorders
    S100 Calcium Binding Protein beta Subunit
    S100 Proteins
    Substantia Nigra
    Tyrosine 3-Monooxygenase

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    12673835

    Citation

    Muramatsu, Yasuko, et al. "Expression of S-100 Protein Is Related to Neuronal Damage in MPTP-treated Mice." Glia, vol. 42, no. 3, 2003, pp. 307-13.
    Muramatsu Y, Kurosaki R, Watanabe H, et al. Expression of S-100 protein is related to neuronal damage in MPTP-treated mice. Glia. 2003;42(3):307-13.
    Muramatsu, Y., Kurosaki, R., Watanabe, H., Michimata, M., Matsubara, M., Imai, Y., & Araki, T. (2003). Expression of S-100 protein is related to neuronal damage in MPTP-treated mice. Glia, 42(3), pp. 307-13.
    Muramatsu Y, et al. Expression of S-100 Protein Is Related to Neuronal Damage in MPTP-treated Mice. Glia. 2003;42(3):307-13. PubMed PMID: 12673835.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Expression of S-100 protein is related to neuronal damage in MPTP-treated mice. AU - Muramatsu,Yasuko, AU - Kurosaki,Rumiko, AU - Watanabe,Hijiri, AU - Michimata,Mari, AU - Matsubara,Mitsunobu, AU - Imai,Yutaka, AU - Araki,Tsutomu, PY - 2003/4/4/pubmed PY - 2003/6/11/medline PY - 2003/4/4/entrez SP - 307 EP - 13 JF - Glia JO - Glia VL - 42 IS - 3 N2 - S-100beta is a calcium-binding protein expressed at high levels in brain and is known as a marker of brain damage. However, little is known about the role of S-100beta protein during neuronal damage caused by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). To determine whether S-100beta protein is induced in glial cells after MPTP treatment, we investigated the expression of S-100 protein immunohistochemically, using MPTP-treated mice. We also examined the change of neurons and glial cells in mice after MPTP treatment. The present study shows that tyrosine hydroxylase (TH) immunoreactivity decreased gradually in the striatum and substantia nigra from 1 day after MPTP treatment. Thereafter, TH-immunopositive cells and fibers decreased in the striatum and substantia nigra at 3 days after MPTP treatment. In contrast, S-100-immunopositive cells and glial fibrillary acidic protein (GFAP)-immunopositive cells increased markedly in the striatum and substantia nigra at 3 days after MPTP treatment. Seven days after MPTP treatment, S-100-immunopositive cells decreased in the striatum and substantia nigra. However, the number of GFAP-immunopositive cells increased in these regions. In double-labeled immunostaining with anti-S-100 and anti-GFAP antibodies, S-100 immunoreactivity was observed only in the GFAP-positive astrocytes. These results provide evidence that astrocytic activation may play a role in the pathogenesis of MPTP-induced degeneration of dopaminergic neurons. Furthermore, the present study demonstrates that S-100 protein is expressed selectively by astrocytes, but not by microglia, after MPTP treatment. These results provide valuable information for the pathogenesis of the acute stage of Parkinson's disease. SN - 0894-1491 UR - https://www.unboundmedicine.com/medline/citation/12673835/Expression_of_S_100_protein_is_related_to_neuronal_damage_in_MPTP_treated_mice_ L2 - https://doi.org/10.1002/glia.10225 DB - PRIME DP - Unbound Medicine ER -