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Chronic angiotensin II inhibition increases levels of calcitonin gene-related peptide mRNA of the dorsal root ganglia in spontaneously hypertensive rats.
Hypertens Res. 2003 Mar; 26(3):257-63.HR

Abstract

We previously reported that the vasodilation mediated by calcitonin gene-related peptide (CGRP)-containing nerves and level of CGRP mRNA in the dorsal root ganglia (DRG) in spontaneously hypertensive rats (SHR) decreased with age, and that the reduced function of CGRP nerves was restored by chronic inhibition of angiotensin II. The present study was performed to investigate the effect of long-term treatment with angiotensin II type-1 receptor antagonists (L-158,809 and olmesartan), an angiotensin converting enzyme inhibitor (temocapril) and hydralazine on levels of CGRP mRNA in DRG of SHR and the contents of CGRP in the mesenteric artery and atrium. The level of CGRP mRNA and degree of CGRP-like immunoreactivities (CGRP-LI) were measured by Northern blot hybridization assay and enzyme-linked immunosorbent assay, respectively. Seven week-treatment of 8 week-old SHR with temocapril (0.005%), L-158,809 (0.001%), olmesartan (0.01%) or hydralazine (0.01%) administered in drinking water significantly lowered the systolic blood pressure of SHR. The level of CGRP mRNA in the DRG of control SHR was significantly lower than that in normotensive Wistar Kyoto rats (WKY), whereas the level of CGRP-LI in the mesenteric artery and atrium of SHR were significantly greater than those in WKY. Treatment of SHR with temocapril, L-158,809, or olmesartan, but not hydralazine, significantly elevated the levels of CGRP mRNA in DRG, markedly increased the level of CGRP-LI in the mesenteric artery, and slightly increased the CGRP-LI level in the atrium. These results suggest that long-term inhibition of angiotensin II restores the reduced expression of CGRP mRNA in DRG and may facilitate neurotransmission of CGRP-containing vasodilator nerves in SHR.

Authors+Show Affiliations

Department of Clinical Pharmaceutical Science, Graduate School of Natural Science and Technology, Okayama University, Tsushima-naka, Okayama, Japan. kawasaki@pheasant.pharm.okayama-u.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12675282

Citation

Kawasaki, Hiromu, et al. "Chronic Angiotensin II Inhibition Increases Levels of Calcitonin Gene-related Peptide mRNA of the Dorsal Root Ganglia in Spontaneously Hypertensive Rats." Hypertension Research : Official Journal of the Japanese Society of Hypertension, vol. 26, no. 3, 2003, pp. 257-63.
Kawasaki H, Inaizumi K, Nakamura A, et al. Chronic angiotensin II inhibition increases levels of calcitonin gene-related peptide mRNA of the dorsal root ganglia in spontaneously hypertensive rats. Hypertens Res. 2003;26(3):257-63.
Kawasaki, H., Inaizumi, K., Nakamura, A., Hobara, N., & Kurosaki, Y. (2003). Chronic angiotensin II inhibition increases levels of calcitonin gene-related peptide mRNA of the dorsal root ganglia in spontaneously hypertensive rats. Hypertension Research : Official Journal of the Japanese Society of Hypertension, 26(3), 257-63.
Kawasaki H, et al. Chronic Angiotensin II Inhibition Increases Levels of Calcitonin Gene-related Peptide mRNA of the Dorsal Root Ganglia in Spontaneously Hypertensive Rats. Hypertens Res. 2003;26(3):257-63. PubMed PMID: 12675282.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Chronic angiotensin II inhibition increases levels of calcitonin gene-related peptide mRNA of the dorsal root ganglia in spontaneously hypertensive rats. AU - Kawasaki,Hiromu, AU - Inaizumi,Keiichi, AU - Nakamura,Arisa, AU - Hobara,Narumi, AU - Kurosaki,Yuji, PY - 2003/4/5/pubmed PY - 2003/12/3/medline PY - 2003/4/5/entrez SP - 257 EP - 63 JF - Hypertension research : official journal of the Japanese Society of Hypertension JO - Hypertens Res VL - 26 IS - 3 N2 - We previously reported that the vasodilation mediated by calcitonin gene-related peptide (CGRP)-containing nerves and level of CGRP mRNA in the dorsal root ganglia (DRG) in spontaneously hypertensive rats (SHR) decreased with age, and that the reduced function of CGRP nerves was restored by chronic inhibition of angiotensin II. The present study was performed to investigate the effect of long-term treatment with angiotensin II type-1 receptor antagonists (L-158,809 and olmesartan), an angiotensin converting enzyme inhibitor (temocapril) and hydralazine on levels of CGRP mRNA in DRG of SHR and the contents of CGRP in the mesenteric artery and atrium. The level of CGRP mRNA and degree of CGRP-like immunoreactivities (CGRP-LI) were measured by Northern blot hybridization assay and enzyme-linked immunosorbent assay, respectively. Seven week-treatment of 8 week-old SHR with temocapril (0.005%), L-158,809 (0.001%), olmesartan (0.01%) or hydralazine (0.01%) administered in drinking water significantly lowered the systolic blood pressure of SHR. The level of CGRP mRNA in the DRG of control SHR was significantly lower than that in normotensive Wistar Kyoto rats (WKY), whereas the level of CGRP-LI in the mesenteric artery and atrium of SHR were significantly greater than those in WKY. Treatment of SHR with temocapril, L-158,809, or olmesartan, but not hydralazine, significantly elevated the levels of CGRP mRNA in DRG, markedly increased the level of CGRP-LI in the mesenteric artery, and slightly increased the CGRP-LI level in the atrium. These results suggest that long-term inhibition of angiotensin II restores the reduced expression of CGRP mRNA in DRG and may facilitate neurotransmission of CGRP-containing vasodilator nerves in SHR. SN - 0916-9636 UR - https://www.unboundmedicine.com/medline/citation/12675282/Chronic_angiotensin_II_inhibition_increases_levels_of_calcitonin_gene_related_peptide_mRNA_of_the_dorsal_root_ganglia_in_spontaneously_hypertensive_rats_ L2 - http://joi.jlc.jst.go.jp/JST.JSTAGE/hypres/26.257?from=PubMed DB - PRIME DP - Unbound Medicine ER -