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[Influenza activity in China from 2000 to 2001].
Zhonghua Liu Xing Bing Xue Za Zhi. 2003 Jan; 24(1):4-8.ZL

Abstract

OBJECTIVE

To understand the epidemics and antigenic drift of influenza viruses in China from 2000 to 2001.

METHODS

The viruses were grown in embryonated hen eggs with 9 - 10 days old. The egg allantoic fluids with influenza viruses were used. Virion RNA was transcribed into cDNA by reverse transcriptase while cDNA amplified by PCR. Products of PCR were purified. RNA sequence analysis was then performed. Finally, phylogenetic analysis of the sequencing data was performed with MegAlign (Version 1.03) and Editseq (Version 3.69) software.

RESULTS

Data from comparison of amino acid sequence on HA1 domain of HA protein molecule between H1N1 viruses isolated in 2001 and A/Shanghai/7/99 (H1N1) strain indicated that there was only one difference of amino acid located at 190 position (antigenic determinant D). However, phylogenetic analysis showed that there were two distinguishable genetically lineages of H1N1 viruses co-circulating in men in China in 2001. Two antigenically distinct genetic lineages of influenza B viruses were still existing in men in China. Most of influenza B viruses were Yamagata-like strain and there were two different amino acid sequences located at 197 and 199 position on HA1 domain of HA protein molecule, between Victoria-like virus isolated and B/Shandong/7/97 strain. When comparing amino acid sequences on HA1 protein domain of H3N2 viruses isolated in 2000 with those of A/Sydeney/5/97 (H3N2) virus, it was revealed that there were 7 - 8 differences of amino acid sequences between them. However, there were four differences related to amino acid sequences on HA1 protein domain between H3N2 viruses isolated in 2000 and in 2001. These results were further demonstrated by analysis of phylogenic tree.

CONCLUSIONS

Influenza was not prevalent in China from 2000 to 2001. The antigenic drifts of H3N2 and B/Victoria-like viruses occurred. Two antigenically distinct genetic lineages of influenza B viruses were still co-circulating in men in China. Two genetically distinct lineages of influenza A (H1N1) virus were also co-circulating in men in China.

Authors+Show Affiliations

Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Preventiong, Beijing 100052, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

12678953

Citation

Zhang, Ye, et al. "[Influenza Activity in China From 2000 to 2001]." Zhonghua Liu Xing Bing Xue Za Zhi = Zhonghua Liuxingbingxue Zazhi, vol. 24, no. 1, 2003, pp. 4-8.
Zhang Y, Li Z, Guo JF, et al. [Influenza activity in China from 2000 to 2001]. Zhonghua Liu Xing Bing Xue Za Zhi. 2003;24(1):4-8.
Zhang, Y., Li, Z., Guo, J. F., Wang, M., Wen, L. Y., & Guo, Y. J. (2003). [Influenza activity in China from 2000 to 2001]. Zhonghua Liu Xing Bing Xue Za Zhi = Zhonghua Liuxingbingxue Zazhi, 24(1), 4-8.
Zhang Y, et al. [Influenza Activity in China From 2000 to 2001]. Zhonghua Liu Xing Bing Xue Za Zhi. 2003;24(1):4-8. PubMed PMID: 12678953.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Influenza activity in China from 2000 to 2001]. AU - Zhang,Ye, AU - Li,Zi, AU - Guo,Jun-feng, AU - Wang,Min, AU - Wen,Le-ying, AU - Guo,Yuan-ji, PY - 2003/4/8/pubmed PY - 2004/11/4/medline PY - 2003/4/8/entrez SP - 4 EP - 8 JF - Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi JO - Zhonghua Liu Xing Bing Xue Za Zhi VL - 24 IS - 1 N2 - OBJECTIVE: To understand the epidemics and antigenic drift of influenza viruses in China from 2000 to 2001. METHODS: The viruses were grown in embryonated hen eggs with 9 - 10 days old. The egg allantoic fluids with influenza viruses were used. Virion RNA was transcribed into cDNA by reverse transcriptase while cDNA amplified by PCR. Products of PCR were purified. RNA sequence analysis was then performed. Finally, phylogenetic analysis of the sequencing data was performed with MegAlign (Version 1.03) and Editseq (Version 3.69) software. RESULTS: Data from comparison of amino acid sequence on HA1 domain of HA protein molecule between H1N1 viruses isolated in 2001 and A/Shanghai/7/99 (H1N1) strain indicated that there was only one difference of amino acid located at 190 position (antigenic determinant D). However, phylogenetic analysis showed that there were two distinguishable genetically lineages of H1N1 viruses co-circulating in men in China in 2001. Two antigenically distinct genetic lineages of influenza B viruses were still existing in men in China. Most of influenza B viruses were Yamagata-like strain and there were two different amino acid sequences located at 197 and 199 position on HA1 domain of HA protein molecule, between Victoria-like virus isolated and B/Shandong/7/97 strain. When comparing amino acid sequences on HA1 protein domain of H3N2 viruses isolated in 2000 with those of A/Sydeney/5/97 (H3N2) virus, it was revealed that there were 7 - 8 differences of amino acid sequences between them. However, there were four differences related to amino acid sequences on HA1 protein domain between H3N2 viruses isolated in 2000 and in 2001. These results were further demonstrated by analysis of phylogenic tree. CONCLUSIONS: Influenza was not prevalent in China from 2000 to 2001. The antigenic drifts of H3N2 and B/Victoria-like viruses occurred. Two antigenically distinct genetic lineages of influenza B viruses were still co-circulating in men in China. Two genetically distinct lineages of influenza A (H1N1) virus were also co-circulating in men in China. SN - 0254-6450 UR - https://www.unboundmedicine.com/medline/citation/12678953/[Influenza_activity_in_China_from_2000_to_2001]_ L2 - https://medlineplus.gov/flu.html DB - PRIME DP - Unbound Medicine ER -