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Molecular investigation of hepatitis E virus infection in patients with acute hepatitis in Kathmandu, Nepal.
J Med Virol. 2003 Feb; 69(2):207-14.JM

Abstract

One hundred fifty-four consecutive patients with sporadic acute hepatitis, who were seen at a city hospital in the Kathmandu valley of Nepal in 1997, were studied. IgM antibodies to hepatitis A virus were detected in four patients (3%), IgM antibodies to hepatitis B core in four patients (3%), hepatitis B surface antigen in 20 (13%), and hepatitis C virus RNA in four patients (3%). IgM antibodies to hepatitis E virus (HEV) (anti-HEV IgM) and HEV RNA were detected in 77 (50%) and 48 (31%), respectively. Consequently, 86 patients (56%) including nine HEV-viremic patients without anti-HEV IgM, were diagnosed with hepatitis E. The cause of hepatitis was not known in 53 patients (34%). All 48 HEV RNA-positive samples were genotyped as 1, and subtyped further as 1a in 17 (35%), 1c in 29 (60%), and mixed infection of 1a and 1c in 2 (4%). A seasonal difference in the prevalence of HEV subtypes was recognized. Before the rainy season (January to July), both 1a and 1c isolates were found: the intrasubtypic difference was up to 9.0% and 1.7%, respectively, in the 412-nucleotide sequence of open reading frame 2. During the rainy season (August), only 1c isolates (n = 17) with 99.5-100% identity were found; 13 of 17 isolates had the same sequence, being identical to the 3 isolates that emerged at the end of July. These results suggest that a particular HEV 1c strain spread widely during the rainy season and was implicated in a small epidemic in the Kathmandu valley in August 1997.

Authors+Show Affiliations

Liver Foundation Nepal, Tripureswor, Kathmandu, Nepal.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12683409

Citation

Shrestha, Santosh Man, et al. "Molecular Investigation of Hepatitis E Virus Infection in Patients With Acute Hepatitis in Kathmandu, Nepal." Journal of Medical Virology, vol. 69, no. 2, 2003, pp. 207-14.
Shrestha SM, Shrestha S, Tsuda F, et al. Molecular investigation of hepatitis E virus infection in patients with acute hepatitis in Kathmandu, Nepal. J Med Virol. 2003;69(2):207-14.
Shrestha, S. M., Shrestha, S., Tsuda, F., Nishizawa, T., Gotanda, Y., Takeda, N., & Okamoto, H. (2003). Molecular investigation of hepatitis E virus infection in patients with acute hepatitis in Kathmandu, Nepal. Journal of Medical Virology, 69(2), 207-14.
Shrestha SM, et al. Molecular Investigation of Hepatitis E Virus Infection in Patients With Acute Hepatitis in Kathmandu, Nepal. J Med Virol. 2003;69(2):207-14. PubMed PMID: 12683409.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular investigation of hepatitis E virus infection in patients with acute hepatitis in Kathmandu, Nepal. AU - Shrestha,Santosh Man, AU - Shrestha,Shobhana, AU - Tsuda,Fumio, AU - Nishizawa,Tsutomu, AU - Gotanda,Yuhko, AU - Takeda,Naokazu, AU - Okamoto,Hiroaki, PY - 2003/4/10/pubmed PY - 2003/6/14/medline PY - 2003/4/10/entrez SP - 207 EP - 14 JF - Journal of medical virology JO - J Med Virol VL - 69 IS - 2 N2 - One hundred fifty-four consecutive patients with sporadic acute hepatitis, who were seen at a city hospital in the Kathmandu valley of Nepal in 1997, were studied. IgM antibodies to hepatitis A virus were detected in four patients (3%), IgM antibodies to hepatitis B core in four patients (3%), hepatitis B surface antigen in 20 (13%), and hepatitis C virus RNA in four patients (3%). IgM antibodies to hepatitis E virus (HEV) (anti-HEV IgM) and HEV RNA were detected in 77 (50%) and 48 (31%), respectively. Consequently, 86 patients (56%) including nine HEV-viremic patients without anti-HEV IgM, were diagnosed with hepatitis E. The cause of hepatitis was not known in 53 patients (34%). All 48 HEV RNA-positive samples were genotyped as 1, and subtyped further as 1a in 17 (35%), 1c in 29 (60%), and mixed infection of 1a and 1c in 2 (4%). A seasonal difference in the prevalence of HEV subtypes was recognized. Before the rainy season (January to July), both 1a and 1c isolates were found: the intrasubtypic difference was up to 9.0% and 1.7%, respectively, in the 412-nucleotide sequence of open reading frame 2. During the rainy season (August), only 1c isolates (n = 17) with 99.5-100% identity were found; 13 of 17 isolates had the same sequence, being identical to the 3 isolates that emerged at the end of July. These results suggest that a particular HEV 1c strain spread widely during the rainy season and was implicated in a small epidemic in the Kathmandu valley in August 1997. SN - 0146-6615 UR - https://www.unboundmedicine.com/medline/citation/12683409/Molecular_investigation_of_hepatitis_E_virus_infection_in_patients_with_acute_hepatitis_in_Kathmandu_Nepal_ DB - PRIME DP - Unbound Medicine ER -