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Threonine aldolase and alanine racemase: novel examples of convergent evolution in the superfamily of vitamin B6-dependent enzymes.
Biochim Biophys Acta. 2003 Apr 11; 1647(1-2):214-9.BB

Abstract

Vitamin B(6)-dependent enzymes may be grouped into five evolutionarily unrelated families, each having a different fold. Within fold type I enzymes, L-threonine aldolase (L-TA) and fungal alanine racemase (AlaRac) belong to a subgroup of structurally and mechanistically closely related proteins, which specialised during evolution to perform different functions. In a previous study, a comparison of the catalytic properties and active site structures of these enzymes suggested that they have a catalytic apparatus with the same basic features. Recently, recombinant D-threonine aldolases (D-TAs) from two bacterial organisms have been characterised, their predicted amino acid sequences showing no significant similarities to any of the known B(6) enzymes. In the present work, a comparative structural analysis suggests that D-TA has an alpha/beta barrel fold and therefore is a fold type III B(6) enzyme, as eukaryotic ornithine decarboxylase (ODC) and bacterial AlaRac. The presence of both TA and AlaRac in two distinct evolutionary unrelated families represents a novel and interesting example of convergent evolution. The independent emergence of the same catalytic properties in families characterised by completely different folds may have not been determined by chance, but by the similar structural features required to catalyse pyridoxal phosphate-dependent aldolase and racemase reactions.

Authors+Show Affiliations

Dipartimento di Scienze Biochimiche A. Rossi Fanelli, Università degli Studi di Roma La Sapienza, Piazzale Aldo Moro, 5, 00185 Rome, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Review

Language

eng

PubMed ID

12686135

Citation

Paiardini, Alessandro, et al. "Threonine Aldolase and Alanine Racemase: Novel Examples of Convergent Evolution in the Superfamily of Vitamin B6-dependent Enzymes." Biochimica Et Biophysica Acta, vol. 1647, no. 1-2, 2003, pp. 214-9.
Paiardini A, Contestabile R, D'Aguanno S, et al. Threonine aldolase and alanine racemase: novel examples of convergent evolution in the superfamily of vitamin B6-dependent enzymes. Biochim Biophys Acta. 2003;1647(1-2):214-9.
Paiardini, A., Contestabile, R., D'Aguanno, S., Pascarella, S., & Bossa, F. (2003). Threonine aldolase and alanine racemase: novel examples of convergent evolution in the superfamily of vitamin B6-dependent enzymes. Biochimica Et Biophysica Acta, 1647(1-2), 214-9.
Paiardini A, et al. Threonine Aldolase and Alanine Racemase: Novel Examples of Convergent Evolution in the Superfamily of Vitamin B6-dependent Enzymes. Biochim Biophys Acta. 2003 Apr 11;1647(1-2):214-9. PubMed PMID: 12686135.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Threonine aldolase and alanine racemase: novel examples of convergent evolution in the superfamily of vitamin B6-dependent enzymes. AU - Paiardini,Alessandro, AU - Contestabile,Roberto, AU - D'Aguanno,Simona, AU - Pascarella,Stefano, AU - Bossa,Francesco, PY - 2003/4/11/pubmed PY - 2003/6/6/medline PY - 2003/4/11/entrez SP - 214 EP - 9 JF - Biochimica et biophysica acta JO - Biochim Biophys Acta VL - 1647 IS - 1-2 N2 - Vitamin B(6)-dependent enzymes may be grouped into five evolutionarily unrelated families, each having a different fold. Within fold type I enzymes, L-threonine aldolase (L-TA) and fungal alanine racemase (AlaRac) belong to a subgroup of structurally and mechanistically closely related proteins, which specialised during evolution to perform different functions. In a previous study, a comparison of the catalytic properties and active site structures of these enzymes suggested that they have a catalytic apparatus with the same basic features. Recently, recombinant D-threonine aldolases (D-TAs) from two bacterial organisms have been characterised, their predicted amino acid sequences showing no significant similarities to any of the known B(6) enzymes. In the present work, a comparative structural analysis suggests that D-TA has an alpha/beta barrel fold and therefore is a fold type III B(6) enzyme, as eukaryotic ornithine decarboxylase (ODC) and bacterial AlaRac. The presence of both TA and AlaRac in two distinct evolutionary unrelated families represents a novel and interesting example of convergent evolution. The independent emergence of the same catalytic properties in families characterised by completely different folds may have not been determined by chance, but by the similar structural features required to catalyse pyridoxal phosphate-dependent aldolase and racemase reactions. SN - 0006-3002 UR - https://www.unboundmedicine.com/medline/citation/12686135/Threonine_aldolase_and_alanine_racemase:_novel_examples_of_convergent_evolution_in_the_superfamily_of_vitamin_B6_dependent_enzymes_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570963903000505 DB - PRIME DP - Unbound Medicine ER -