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Localization of GABAA receptor subunits alpha 1, alpha 3, beta 1, beta 2/3, gamma 1, and gamma 2 in the salamander retina.
J Comp Neurol 2003; 459(4):440-53JC

Abstract

Electrophysiological studies have demonstrated that gamma-aminobutyric acid receptors type A (GABA(A)) mediate important information processing in the retinas of salamander and other vertebrates. The pharmacology and physiology of GABA(A) receptors depend on their subunit composition. We studied the localization of GABA(A) receptor subunit isoforms alpha(1), alpha(3), beta(1), beta(2/3) (antibody BD-17 and 62-3G1), gamma(1), and gamma(2) in salamander retina with immunocytochemical methods. All three beta-subunit antibodies labeled similarly in the outer retina, especially the inner segments and synaptic terminals of rod photoreceptors (identified with protein kinase C). Somatic labeling was observed in cell bodies of some horizontal cells, bipolar cells, amacrine cells, and cells in the ganglion cell layer (GCL). Puncta were present throughout the inner plexiform layer (IPL) for beta(1) and 62-3G1, but not for BD-17. alpha(1)-immunoreactivity (IR) stained a population of presumed OFF rod-dominated bipolar cells, including dendrites, soma, and axon terminals in the distal IPL. A subtype of GABAergic amacrine cell also expressed alpha(1)-IR, with puncta sparsely distributed at the distal and proximal margins of the IPL. Both the OPL and IPL were labeled throughout for alpha(3)-IR, as opposed to the narrow distribution of alpha(1)-IR in the IPL, suggesting that the two alpha-subunits are localized at different synaptic sites. Punctate gamma(1)-IR was observed in the OPL and IPL, whereas gamma(2) was most prominent in cone photoreceptors (identified with calbindin), including the terminal telodendria, in cell bodies of some horizontal cells, amacrine cells, cells in the GCL, and less intensely in the IPL. In addition, several subunits were present in Müller cells. The differential labeling suggests the existence of GABA(A) receptor subtypes with different subunit compositions that mediate multiple GABAergic functions in salamander retina.

Authors+Show Affiliations

Department of Neurobiology and Behavior, State University of New York at Stony Brook, 11794, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

12687709

Citation

Zhang, Jun, et al. "Localization of GABAA Receptor Subunits Alpha 1, Alpha 3, Beta 1, Beta 2/3, Gamma 1, and Gamma 2 in the Salamander Retina." The Journal of Comparative Neurology, vol. 459, no. 4, 2003, pp. 440-53.
Zhang J, De Blas AL, Miralles CP, et al. Localization of GABAA receptor subunits alpha 1, alpha 3, beta 1, beta 2/3, gamma 1, and gamma 2 in the salamander retina. J Comp Neurol. 2003;459(4):440-53.
Zhang, J., De Blas, A. L., Miralles, C. P., & Yang, C. Y. (2003). Localization of GABAA receptor subunits alpha 1, alpha 3, beta 1, beta 2/3, gamma 1, and gamma 2 in the salamander retina. The Journal of Comparative Neurology, 459(4), pp. 440-53.
Zhang J, et al. Localization of GABAA Receptor Subunits Alpha 1, Alpha 3, Beta 1, Beta 2/3, Gamma 1, and Gamma 2 in the Salamander Retina. J Comp Neurol. 2003 May 12;459(4):440-53. PubMed PMID: 12687709.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Localization of GABAA receptor subunits alpha 1, alpha 3, beta 1, beta 2/3, gamma 1, and gamma 2 in the salamander retina. AU - Zhang,Jun, AU - De Blas,Angel L, AU - Miralles,Celia P, AU - Yang,Chen-Yu, PY - 2003/4/11/pubmed PY - 2003/6/5/medline PY - 2003/4/11/entrez SP - 440 EP - 53 JF - The Journal of comparative neurology JO - J. Comp. Neurol. VL - 459 IS - 4 N2 - Electrophysiological studies have demonstrated that gamma-aminobutyric acid receptors type A (GABA(A)) mediate important information processing in the retinas of salamander and other vertebrates. The pharmacology and physiology of GABA(A) receptors depend on their subunit composition. We studied the localization of GABA(A) receptor subunit isoforms alpha(1), alpha(3), beta(1), beta(2/3) (antibody BD-17 and 62-3G1), gamma(1), and gamma(2) in salamander retina with immunocytochemical methods. All three beta-subunit antibodies labeled similarly in the outer retina, especially the inner segments and synaptic terminals of rod photoreceptors (identified with protein kinase C). Somatic labeling was observed in cell bodies of some horizontal cells, bipolar cells, amacrine cells, and cells in the ganglion cell layer (GCL). Puncta were present throughout the inner plexiform layer (IPL) for beta(1) and 62-3G1, but not for BD-17. alpha(1)-immunoreactivity (IR) stained a population of presumed OFF rod-dominated bipolar cells, including dendrites, soma, and axon terminals in the distal IPL. A subtype of GABAergic amacrine cell also expressed alpha(1)-IR, with puncta sparsely distributed at the distal and proximal margins of the IPL. Both the OPL and IPL were labeled throughout for alpha(3)-IR, as opposed to the narrow distribution of alpha(1)-IR in the IPL, suggesting that the two alpha-subunits are localized at different synaptic sites. Punctate gamma(1)-IR was observed in the OPL and IPL, whereas gamma(2) was most prominent in cone photoreceptors (identified with calbindin), including the terminal telodendria, in cell bodies of some horizontal cells, amacrine cells, cells in the GCL, and less intensely in the IPL. In addition, several subunits were present in Müller cells. The differential labeling suggests the existence of GABA(A) receptor subtypes with different subunit compositions that mediate multiple GABAergic functions in salamander retina. SN - 0021-9967 UR - https://www.unboundmedicine.com/medline/citation/12687709/Localization_of_GABAA_receptor_subunits_alpha_1_alpha_3_beta_1_beta_2/3_gamma_1_and_gamma_2_in_the_salamander_retina_ L2 - https://doi.org/10.1002/cne.10626 DB - PRIME DP - Unbound Medicine ER -