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Cellular defense against singlet oxygen-induced oxidative damage by cytosolic NADP+-dependent isocitrate dehydrogenase.
Free Radic Res. 2003 Mar; 37(3):309-16.FR

Abstract

Singlet oxygen (1O2) is a highly reactive form of molecular oxygen that may harm living systems by oxidizing critical cellular macromolecules. Recently, we have shown that NADP+-dependent isocitrate dehydrogenase is involved in the supply of NADPH needed for GSH production against cellular oxidative damage. In this study, we investigated the role of cytosolic form of NADP+-dependent isocitrate dehydrogenase (IDPc) against singlet oxygen-induced cytotoxicity by comparing the relative degree of cellular responses in three different NIH3T3 cells with stable transfection with the cDNA for mouse IDPc in sense and antisense orientations, where IDPc activities were 2.3-fold higher and 39% lower, respectively, than that in the parental cells carrying the vector alone. Upon exposure to singlet oxygen generated from photoactivated dye, the cells with low levels of IDPc became more sensitive to cell killing. Lipid peroxidation, protein oxidation, oxidative DNA damage and intracellular peroxide generation were higher in the cell-line expressing the lower level of IDPc. However, the cells with the highly over-expressed IDPc exhibited enhanced resistance against singlet oxygen, compared to the control cells. The data indicate that IDPc plays an important role in cellular defense against singlet oxygen-induced oxidative injury.

Authors+Show Affiliations

Department of Biochemistry, College of Natural Sciences, Kyungpook National University, Taegu, South Korea.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12688426

Citation

Kim, Sun Yee, and Jeen-Woo Park. "Cellular Defense Against Singlet Oxygen-induced Oxidative Damage By Cytosolic NADP+-dependent Isocitrate Dehydrogenase." Free Radical Research, vol. 37, no. 3, 2003, pp. 309-16.
Kim SY, Park JW. Cellular defense against singlet oxygen-induced oxidative damage by cytosolic NADP+-dependent isocitrate dehydrogenase. Free Radic Res. 2003;37(3):309-16.
Kim, S. Y., & Park, J. W. (2003). Cellular defense against singlet oxygen-induced oxidative damage by cytosolic NADP+-dependent isocitrate dehydrogenase. Free Radical Research, 37(3), 309-16.
Kim SY, Park JW. Cellular Defense Against Singlet Oxygen-induced Oxidative Damage By Cytosolic NADP+-dependent Isocitrate Dehydrogenase. Free Radic Res. 2003;37(3):309-16. PubMed PMID: 12688426.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cellular defense against singlet oxygen-induced oxidative damage by cytosolic NADP+-dependent isocitrate dehydrogenase. AU - Kim,Sun Yee, AU - Park,Jeen-Woo, PY - 2003/4/12/pubmed PY - 2003/9/10/medline PY - 2003/4/12/entrez SP - 309 EP - 16 JF - Free radical research JO - Free Radic Res VL - 37 IS - 3 N2 - Singlet oxygen (1O2) is a highly reactive form of molecular oxygen that may harm living systems by oxidizing critical cellular macromolecules. Recently, we have shown that NADP+-dependent isocitrate dehydrogenase is involved in the supply of NADPH needed for GSH production against cellular oxidative damage. In this study, we investigated the role of cytosolic form of NADP+-dependent isocitrate dehydrogenase (IDPc) against singlet oxygen-induced cytotoxicity by comparing the relative degree of cellular responses in three different NIH3T3 cells with stable transfection with the cDNA for mouse IDPc in sense and antisense orientations, where IDPc activities were 2.3-fold higher and 39% lower, respectively, than that in the parental cells carrying the vector alone. Upon exposure to singlet oxygen generated from photoactivated dye, the cells with low levels of IDPc became more sensitive to cell killing. Lipid peroxidation, protein oxidation, oxidative DNA damage and intracellular peroxide generation were higher in the cell-line expressing the lower level of IDPc. However, the cells with the highly over-expressed IDPc exhibited enhanced resistance against singlet oxygen, compared to the control cells. The data indicate that IDPc plays an important role in cellular defense against singlet oxygen-induced oxidative injury. SN - 1071-5762 UR - https://www.unboundmedicine.com/medline/citation/12688426/Cellular_defense_against_singlet_oxygen_induced_oxidative_damage_by_cytosolic_NADP+_dependent_isocitrate_dehydrogenase_ L2 - https://www.tandfonline.com/doi/full/10.1080/1071576021000050429 DB - PRIME DP - Unbound Medicine ER -