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A specific peroxisome proliferator-activated receptor-gamma (PPAR-gamma) ligand, pioglitazone, ameliorates gastric mucosal damage induced by ischemia and reperfusion in rats.
Redox Rep. 2002; 7(5):343-6.RR

Abstract

Peroxisome proliferator-activated receptor-gamma (PPAR-gamma), a member of the nuclear hormone receptor superfamily, has recently been implicated as a regulator of cellular proliferation and inflammatory responses. The aim of the present study was to investigate the effects of pioglitazone on ischemia-reperfusion (I/R)-induced gastric mucosal injury in rats. Gastric ischemia was induced for 30 min by applying a small vascular clamp to the celiac artery and reperfusion was produced by removal of the clamp in male Sprague-Dawley rats treated with and without pioglitazone. Pioglitazone was given to the rats intraperitoneally 2 h before the vascular clamping. The area of gastric mucosal erosion (erosion index) significantly increased from mean basal levels after 60 min of reperfusion. This erosion index was significantly inhibited by pretreatment with pioglitazone in a dose-dependent manner. The concentration of thiobarbituric acid reactive substances (TBARS) and myeloperoxidase (MPO) activity in the gastric mucosa were both significantly increased after I/R, and pretreatment with pioglitazone significantly reduced these increases. The contents of both mucosal TNF-alpha and CINC-2beta in the I/R group were significantly increased compared with the levels in the sham-operated group. These increases in TNF-alpha and CINC-2beta were significantly inhibited by pretreatment with pioglitazone at a dose of 10 mg/kg. The results of the present study indicate that pioglitazone inhibited lipid peroxidation and reduced development of the gastric mucosal inflammation induced by I/R in rats. This investigation suggests that pioglitazone has potential as a new therapeutic agent for reperfusion injury.

Authors+Show Affiliations

First Department of Internal Medicine, Kyoto Prefectural University of Medicine, Kyoto, Japan. hichik@koto.kpu-m.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

12688526

Citation

Ichikawa, Hiroshi, et al. "A Specific Peroxisome Proliferator-activated Receptor-gamma (PPAR-gamma) Ligand, Pioglitazone, Ameliorates Gastric Mucosal Damage Induced By Ischemia and Reperfusion in Rats." Redox Report : Communications in Free Radical Research, vol. 7, no. 5, 2002, pp. 343-6.
Ichikawa H, Naito Y, Takagi T, et al. A specific peroxisome proliferator-activated receptor-gamma (PPAR-gamma) ligand, pioglitazone, ameliorates gastric mucosal damage induced by ischemia and reperfusion in rats. Redox Rep. 2002;7(5):343-6.
Ichikawa, H., Naito, Y., Takagi, T., Tomatsuri, N., Yoshida, N., & Yoshikawa, T. (2002). A specific peroxisome proliferator-activated receptor-gamma (PPAR-gamma) ligand, pioglitazone, ameliorates gastric mucosal damage induced by ischemia and reperfusion in rats. Redox Report : Communications in Free Radical Research, 7(5), 343-6.
Ichikawa H, et al. A Specific Peroxisome Proliferator-activated Receptor-gamma (PPAR-gamma) Ligand, Pioglitazone, Ameliorates Gastric Mucosal Damage Induced By Ischemia and Reperfusion in Rats. Redox Rep. 2002;7(5):343-6. PubMed PMID: 12688526.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A specific peroxisome proliferator-activated receptor-gamma (PPAR-gamma) ligand, pioglitazone, ameliorates gastric mucosal damage induced by ischemia and reperfusion in rats. AU - Ichikawa,Hiroshi, AU - Naito,Yuji, AU - Takagi,Tomohisa, AU - Tomatsuri,Naoya, AU - Yoshida,Norimasa, AU - Yoshikawa,Toshikazu, PY - 2003/4/12/pubmed PY - 2003/8/26/medline PY - 2003/4/12/entrez SP - 343 EP - 6 JF - Redox report : communications in free radical research JO - Redox Rep VL - 7 IS - 5 N2 - Peroxisome proliferator-activated receptor-gamma (PPAR-gamma), a member of the nuclear hormone receptor superfamily, has recently been implicated as a regulator of cellular proliferation and inflammatory responses. The aim of the present study was to investigate the effects of pioglitazone on ischemia-reperfusion (I/R)-induced gastric mucosal injury in rats. Gastric ischemia was induced for 30 min by applying a small vascular clamp to the celiac artery and reperfusion was produced by removal of the clamp in male Sprague-Dawley rats treated with and without pioglitazone. Pioglitazone was given to the rats intraperitoneally 2 h before the vascular clamping. The area of gastric mucosal erosion (erosion index) significantly increased from mean basal levels after 60 min of reperfusion. This erosion index was significantly inhibited by pretreatment with pioglitazone in a dose-dependent manner. The concentration of thiobarbituric acid reactive substances (TBARS) and myeloperoxidase (MPO) activity in the gastric mucosa were both significantly increased after I/R, and pretreatment with pioglitazone significantly reduced these increases. The contents of both mucosal TNF-alpha and CINC-2beta in the I/R group were significantly increased compared with the levels in the sham-operated group. These increases in TNF-alpha and CINC-2beta were significantly inhibited by pretreatment with pioglitazone at a dose of 10 mg/kg. The results of the present study indicate that pioglitazone inhibited lipid peroxidation and reduced development of the gastric mucosal inflammation induced by I/R in rats. This investigation suggests that pioglitazone has potential as a new therapeutic agent for reperfusion injury. SN - 1351-0002 UR - https://www.unboundmedicine.com/medline/citation/12688526/A_specific_peroxisome_proliferator_activated_receptor_gamma__PPAR_gamma__ligand_pioglitazone_ameliorates_gastric_mucosal_damage_induced_by_ischemia_and_reperfusion_in_rats_ DB - PRIME DP - Unbound Medicine ER -