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Expression of a thioredoxin peroxidase in insulin-producing cells.
Diabetes Metab. 2002 Dec; 28(6 Pt 2):3S25-8; discussion 3S108-12.DM

Abstract

The presence of thioredoxin peroxidase (TPx), also known as thiol specific antioxidant (TSA), was investigated in neonatal and adult rat islets, and in the beta-cell line HIT-T15. Western blotting of extracts from neonatal and adult pancreatic islets and from the tumoral cell line HIT-T15 revealed the presence of a 25 kDa protein that comigrated with purified yeast TPx. Endocrine pancreatic TPx accounted for approximately 0.01% of the total protein content. Treatment with H2O2 for 3 h increased the expression of TPx in HIT-T15 cells. The distribution of TPx throughout the islet cells was confirmed by immunocytochemistry. Since pancreatic beta-cells possess a weak antioxidant enzyme defense system, especially with regard to hydrogen peroxidase-decomposing enzymes, the presence of a TPx analog in islets suggests that this enzyme may play a role in protecting pancreatic cells against reactive oxygen species.

Authors+Show Affiliations

Boschero AC
Department of Physiology and Biophysics, Institute of Biology, State University of Campinas, SP, Brazil. boschero@unicamp.br
Stoppiglia LF
No affiliation info available
Collares-Buzato CB
No affiliation info available
Bosqueiro JR
No affiliation info available
Delghingaro-Augusto V
No affiliation info available
Leite A
No affiliation info available
Carvalho CP
No affiliation info available
Netto LE
No affiliation info available
Carneiro EM
No affiliation info available

MeSH

AgingAnimalsAnimals, NewbornCells, CulturedInsulinomaIslets of LangerhansNeoplasm ProteinsOxidative StressPancreatic NeoplasmsPeroxidasesPeroxiredoxinsRatsRats, WistarTumor Cells, Cultured

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12688630

Citation

Boschero, A C., et al. "Expression of a Thioredoxin Peroxidase in Insulin-producing Cells." Diabetes & Metabolism, vol. 28, no. 6 Pt 2, 2002, pp. 3S25-8; discussion 3S108-12.
Boschero AC, Stoppiglia LF, Collares-Buzato CB, et al. Expression of a thioredoxin peroxidase in insulin-producing cells. Diabetes Metab. 2002;28(6 Pt 2):3S25-8; discussion 3S108-12.
Boschero, A. C., Stoppiglia, L. F., Collares-Buzato, C. B., Bosqueiro, J. R., Delghingaro-Augusto, V., Leite, A., Carvalho, C. P., Netto, L. E., & Carneiro, E. M. (2002). Expression of a thioredoxin peroxidase in insulin-producing cells. Diabetes & Metabolism, 28(6 Pt 2), 3S25-8; discussion 3S108-12.
Boschero AC, et al. Expression of a Thioredoxin Peroxidase in Insulin-producing Cells. Diabetes Metab. 2002;28(6 Pt 2):3S25-8; discussion 3S108-12. PubMed PMID: 12688630.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression of a thioredoxin peroxidase in insulin-producing cells. AU - Boschero,A C, AU - Stoppiglia,L F, AU - Collares-Buzato,C B, AU - Bosqueiro,J R, AU - Delghingaro-Augusto,V, AU - Leite,A, AU - Carvalho,C P F, AU - Netto,L E S, AU - Carneiro,E M, PY - 2003/4/12/pubmed PY - 2003/7/8/medline PY - 2003/4/12/entrez SP - 3S25-8; discussion 3S108-12 JF - Diabetes & metabolism JO - Diabetes Metab VL - 28 IS - 6 Pt 2 N2 - The presence of thioredoxin peroxidase (TPx), also known as thiol specific antioxidant (TSA), was investigated in neonatal and adult rat islets, and in the beta-cell line HIT-T15. Western blotting of extracts from neonatal and adult pancreatic islets and from the tumoral cell line HIT-T15 revealed the presence of a 25 kDa protein that comigrated with purified yeast TPx. Endocrine pancreatic TPx accounted for approximately 0.01% of the total protein content. Treatment with H2O2 for 3 h increased the expression of TPx in HIT-T15 cells. The distribution of TPx throughout the islet cells was confirmed by immunocytochemistry. Since pancreatic beta-cells possess a weak antioxidant enzyme defense system, especially with regard to hydrogen peroxidase-decomposing enzymes, the presence of a TPx analog in islets suggests that this enzyme may play a role in protecting pancreatic cells against reactive oxygen species. SN - 1262-3636 UR - https://www.unboundmedicine.com/medline/citation/12688630/Expression_of_a_thioredoxin_peroxidase_in_insulin_producing_cells_ DB - PRIME DP - Unbound Medicine ER -