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Identification of a novel coronavirus in patients with severe acute respiratory syndrome.
N Engl J Med. 2003 May 15; 348(20):1967-76.NEJM

Abstract

BACKGROUND

The severe acute respiratory syndrome (SARS) has recently been identified as a new clinical entity. SARS is thought to be caused by an unknown infectious agent.

METHODS

Clinical specimens from patients with SARS were searched for unknown viruses with the use of cell cultures and molecular techniques.

RESULTS

A novel coronavirus was identified in patients with SARS. The virus was isolated in cell culture, and a sequence 300 nucleotides in length was obtained by a polymerase-chain-reaction (PCR)-based random-amplification procedure. Genetic characterization indicated that the virus is only distantly related to known coronaviruses (identical in 50 to 60 percent of the nucleotide sequence). On the basis of the obtained sequence, conventional and real-time PCR assays for specific and sensitive detection of the novel virus were established. Virus was detected in a variety of clinical specimens from patients with SARS but not in controls. High concentrations of viral RNA of up to 100 million molecules per milliliter were found in sputum. Viral RNA was also detected at extremely low concentrations in plasma during the acute phase and in feces during the late convalescent phase. Infected patients showed seroconversion on the Vero cells in which the virus was isolated.

CONCLUSIONS

The novel coronavirus might have a role in causing SARS.

Authors+Show Affiliations

Drosten C
Bernhard Nocht Institute for Tropical Medicine, National Reference Center for Tropical Infectious Diseases, Hamburg, Germany. drosten@bni-hamburg.de
Günther S
No affiliation info available
Preiser W
No affiliation info available
van der Werf S
No affiliation info available
Brodt HR
No affiliation info available
Becker S
No affiliation info available
Rabenau H
No affiliation info available
Panning M
No affiliation info available
Kolesnikova L
No affiliation info available
Fouchier RA
No affiliation info available
Berger A
No affiliation info available
Burguière AM
No affiliation info available
Cinatl J
No affiliation info available
Eickmann M
No affiliation info available
Escriou N
No affiliation info available
Grywna K
No affiliation info available
Kramme S
No affiliation info available
Manuguerra JC
No affiliation info available
Müller S
No affiliation info available
Rickerts V
No affiliation info available
Stürmer M
No affiliation info available
Vieth S
No affiliation info available
Klenk HD
No affiliation info available
Osterhaus AD
No affiliation info available
Schmitz H
No affiliation info available
Doerr HW
No affiliation info available

MeSH

AdultAmino Acid SequenceAnimalsBase SequenceCattleCoronavirusDNA, ViralDisease OutbreaksFemaleHumansMaleMolecular Sequence DataPhylogenyPolymerase Chain ReactionRNA, ViralSevere acute respiratory syndrome-related coronavirusSequence Homology, Nucleic AcidSevere Acute Respiratory SyndromeSputum

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12690091

Clinical Trial Links

Efficacy and Safety of IFN-α2β in the Treatment of Novel Coronavirus Patients
European/Euro-ELSO Survey on Adult and Neonatal/ Pediatric COVID-19 Patients in ECMO

Citation

Drosten, Christian, et al. "Identification of a Novel Coronavirus in Patients With Severe Acute Respiratory Syndrome." The New England Journal of Medicine, vol. 348, no. 20, 2003, pp. 1967-76.
Drosten C, Günther S, Preiser W, et al. Identification of a novel coronavirus in patients with severe acute respiratory syndrome. N Engl J Med. 2003;348(20):1967-76.
Drosten, C., Günther, S., Preiser, W., van der Werf, S., Brodt, H. R., Becker, S., Rabenau, H., Panning, M., Kolesnikova, L., Fouchier, R. A., Berger, A., Burguière, A. M., Cinatl, J., Eickmann, M., Escriou, N., Grywna, K., Kramme, S., Manuguerra, J. C., Müller, S., ... Doerr, H. W. (2003). Identification of a novel coronavirus in patients with severe acute respiratory syndrome. The New England Journal of Medicine, 348(20), 1967-76.
Drosten C, et al. Identification of a Novel Coronavirus in Patients With Severe Acute Respiratory Syndrome. N Engl J Med. 2003 May 15;348(20):1967-76. PubMed PMID: 12690091.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of a novel coronavirus in patients with severe acute respiratory syndrome. AU - Drosten,Christian, AU - Günther,Stephan, AU - Preiser,Wolfgang, AU - van der Werf,Sylvie, AU - Brodt,Hans-Reinhard, AU - Becker,Stephan, AU - Rabenau,Holger, AU - Panning,Marcus, AU - Kolesnikova,Larissa, AU - Fouchier,Ron A M, AU - Berger,Annemarie, AU - Burguière,Ana-Maria, AU - Cinatl,Jindrich, AU - Eickmann,Markus, AU - Escriou,Nicolas, AU - Grywna,Klaus, AU - Kramme,Stefanie, AU - Manuguerra,Jean-Claude, AU - Müller,Stefanie, AU - Rickerts,Volker, AU - Stürmer,Martin, AU - Vieth,Simon, AU - Klenk,Hans-Dieter, AU - Osterhaus,Albert D M E, AU - Schmitz,Herbert, AU - Doerr,Hans Wilhelm, Y1 - 2003/04/10/ PY - 2003/4/12/pubmed PY - 2003/5/22/medline PY - 2003/4/12/entrez SP - 1967 EP - 76 JF - The New England journal of medicine JO - N Engl J Med VL - 348 IS - 20 N2 - BACKGROUND: The severe acute respiratory syndrome (SARS) has recently been identified as a new clinical entity. SARS is thought to be caused by an unknown infectious agent. METHODS: Clinical specimens from patients with SARS were searched for unknown viruses with the use of cell cultures and molecular techniques. RESULTS: A novel coronavirus was identified in patients with SARS. The virus was isolated in cell culture, and a sequence 300 nucleotides in length was obtained by a polymerase-chain-reaction (PCR)-based random-amplification procedure. Genetic characterization indicated that the virus is only distantly related to known coronaviruses (identical in 50 to 60 percent of the nucleotide sequence). On the basis of the obtained sequence, conventional and real-time PCR assays for specific and sensitive detection of the novel virus were established. Virus was detected in a variety of clinical specimens from patients with SARS but not in controls. High concentrations of viral RNA of up to 100 million molecules per milliliter were found in sputum. Viral RNA was also detected at extremely low concentrations in plasma during the acute phase and in feces during the late convalescent phase. Infected patients showed seroconversion on the Vero cells in which the virus was isolated. CONCLUSIONS: The novel coronavirus might have a role in causing SARS. SN - 1533-4406 UR - https://www.unboundmedicine.com/medline/citation/12690091/Identification_of_a_novel_coronavirus_in_patients_with_severe_acute_respiratory_syndrome_ DB - PRIME DP - Unbound Medicine ER -