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Intervention with nitric oxide synthase inhibitors for traumatic shock in rats.
Di Yi Jun Yi Da Xue Xue Bao. 2003 Apr; 23(4):306-9.DY

Abstract

OBJECTIVE

To evaluate the effects of a selective inhibitor of inducible nitric oxide synthase (iNOS) aminoguanidine (AG) and a non-selective inhibitor of nitric oxide synthase (NOS) N(G)-nitro-L-arginine methylester (L-NAME) on traumatic shock in rats.

METHODS

Animal models of traumatic shock were established in 44 Sprague-Dawley rats following fractures in both femur shafts and subsequent depletion until the mean arterial pressure in the femoral artery dropped to 35 to 45 mmHg(4.67-6.00 kPa). Hypotension was maintained for 30 min before the collected blood was infused back into the rats supplemented with Ringer's solution of the same volume. The rat models were then randomly divided into 3 groups, namely traumatic shock group (n=10), AG group (which was subdivided into AGI, AGII, and AGIII groups, each consisting of 8 rats and receiving 2, 8, and 60 mg/kg x b.w AG infusion respectively during resuscitation), and L-NAME group (with 8 mg/kg x b.w L-NAME infusion during resuscitation, n=10). Plasma NO levels were determined before and after shock, immediately after resuscitation and 0.5, 2, 4 h after resuscitation, and the survival rates within 24 h were recorded with tissue samples of the lung, liver, kidney and intestine obtained 24 h after shock for microscopic examination.

RESULTS

Plasma NO level was seen to increase markedly after traumatic shock in the rat models. In the 3 AG groups, the elevated NO levels following the shock were obviously reduced after resuscitation with less tissue damages and higher survival rates, as compared with the other 2 groups. The best protective effect against traumatic shock was observed in AGIII group. In spite of obvious plasma NO level-lowering effect after resuscitation, L-NAME exhibited little efficacy in alleviating the tissue damages in the organs and hence failed to improve the survival rate of the rats.

CONCLUSIONS

NO plays an important role in the pathological process of traumatic shock, and the application of AG may improve the condition. L-NAME can decrease plasma NO level after resuscitation, but fail to improve the outcome of traumatic shock in rats.

Authors+Show Affiliations

Department of General Surgery, Zhujiang Hospital, First Military Medical University, Guangzhou 510282, China. sungaobin5278@sina.comNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12697459

Citation

Sun, Gao-bin, et al. "Intervention With Nitric Oxide Synthase Inhibitors for Traumatic Shock in Rats." Di 1 Jun Yi Da Xue Xue Bao = Academic Journal of the First Medical College of PLA, vol. 23, no. 4, 2003, pp. 306-9.
Sun GB, Huang ZH, Sun YG, et al. Intervention with nitric oxide synthase inhibitors for traumatic shock in rats. Di Yi Jun Yi Da Xue Xue Bao. 2003;23(4):306-9.
Sun, G. B., Huang, Z. H., Sun, Y. G., & Yang, W. Y. (2003). Intervention with nitric oxide synthase inhibitors for traumatic shock in rats. Di 1 Jun Yi Da Xue Xue Bao = Academic Journal of the First Medical College of PLA, 23(4), 306-9.
Sun GB, et al. Intervention With Nitric Oxide Synthase Inhibitors for Traumatic Shock in Rats. Di Yi Jun Yi Da Xue Xue Bao. 2003;23(4):306-9. PubMed PMID: 12697459.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Intervention with nitric oxide synthase inhibitors for traumatic shock in rats. AU - Sun,Gao-bin, AU - Huang,Zong-hai, AU - Sun,Ying-gang, AU - Yang,Wen-yu, PY - 2003/4/17/pubmed PY - 2003/12/3/medline PY - 2003/4/17/entrez SP - 306 EP - 9 JF - Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA JO - Di Yi Jun Yi Da Xue Xue Bao VL - 23 IS - 4 N2 - OBJECTIVE: To evaluate the effects of a selective inhibitor of inducible nitric oxide synthase (iNOS) aminoguanidine (AG) and a non-selective inhibitor of nitric oxide synthase (NOS) N(G)-nitro-L-arginine methylester (L-NAME) on traumatic shock in rats. METHODS: Animal models of traumatic shock were established in 44 Sprague-Dawley rats following fractures in both femur shafts and subsequent depletion until the mean arterial pressure in the femoral artery dropped to 35 to 45 mmHg(4.67-6.00 kPa). Hypotension was maintained for 30 min before the collected blood was infused back into the rats supplemented with Ringer's solution of the same volume. The rat models were then randomly divided into 3 groups, namely traumatic shock group (n=10), AG group (which was subdivided into AGI, AGII, and AGIII groups, each consisting of 8 rats and receiving 2, 8, and 60 mg/kg x b.w AG infusion respectively during resuscitation), and L-NAME group (with 8 mg/kg x b.w L-NAME infusion during resuscitation, n=10). Plasma NO levels were determined before and after shock, immediately after resuscitation and 0.5, 2, 4 h after resuscitation, and the survival rates within 24 h were recorded with tissue samples of the lung, liver, kidney and intestine obtained 24 h after shock for microscopic examination. RESULTS: Plasma NO level was seen to increase markedly after traumatic shock in the rat models. In the 3 AG groups, the elevated NO levels following the shock were obviously reduced after resuscitation with less tissue damages and higher survival rates, as compared with the other 2 groups. The best protective effect against traumatic shock was observed in AGIII group. In spite of obvious plasma NO level-lowering effect after resuscitation, L-NAME exhibited little efficacy in alleviating the tissue damages in the organs and hence failed to improve the survival rate of the rats. CONCLUSIONS: NO plays an important role in the pathological process of traumatic shock, and the application of AG may improve the condition. L-NAME can decrease plasma NO level after resuscitation, but fail to improve the outcome of traumatic shock in rats. SN - 1000-2588 UR - https://www.unboundmedicine.com/medline/citation/12697459/Intervention_with_nitric_oxide_synthase_inhibitors_for_traumatic_shock_in_rats_ DB - PRIME DP - Unbound Medicine ER -