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Normal production of inflammatory cytokines in chronic fatigue and fibromyalgia syndromes determined by intracellular cytokine staining in short-term cultured blood mononuclear cells.
Clin Exp Immunol 2003; 132(2):360-5CE

Abstract

It has been proposed that cytokines play a role in the pathogenesis of chronic fatigue syndrome (CFS) and fibromyalgia syndrome (FMS). However, different studies have reported conflicting results using enzyme-linked immunosorbent assay or polymerase chain reaction to detect cytokines in these conditions. In the present study, for the first time, the production of inflammatory [interleukin (IL)-1alpha, IL-6, and TNF-alpha] and anti-inflammatory (IL-10) cytokines by CD14+ and CD14- peripheral blood mononuclear cells (PBMC) from chronic fatigue syndrome (CFS) and fibromyalgia syndrome (FMS) patients and sex- and age-matched normal subjects was investigated at the level of individual cells using the technique of intracellular cytokine staining and flow cytometry. Cultures were carried out in the presence of polymyxin B to inhibit the effect of endotoxins on cytokine production by monocytes. The mean intensity of fluorescence (MIF) and percentage of CD14+ (monocytes) and CD14- (lymphocytes) cytokine-producing mononuclear cells were comparable in patients and controls in either unstimulated or IFN-gamma-stimulated conditions. Our study indicates that dysregulation of cytokine production by circulating monocytes or non-monocytic cells (lymphocytes) is not a dominant factor in the pathogenesis of CFS/FMS.

Authors+Show Affiliations

Division of Molecular and Clinical Immunology, School of Clinical Laboratory Sciences, University of Nottingham, Queen's Medical Centre, Nottingham, UK.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12699429

Citation

Amel Kashipaz, M R., et al. "Normal Production of Inflammatory Cytokines in Chronic Fatigue and Fibromyalgia Syndromes Determined By Intracellular Cytokine Staining in Short-term Cultured Blood Mononuclear Cells." Clinical and Experimental Immunology, vol. 132, no. 2, 2003, pp. 360-5.
Amel Kashipaz MR, Swinden D, Todd I, et al. Normal production of inflammatory cytokines in chronic fatigue and fibromyalgia syndromes determined by intracellular cytokine staining in short-term cultured blood mononuclear cells. Clin Exp Immunol. 2003;132(2):360-5.
Amel Kashipaz, M. R., Swinden, D., Todd, I., & Powell, R. J. (2003). Normal production of inflammatory cytokines in chronic fatigue and fibromyalgia syndromes determined by intracellular cytokine staining in short-term cultured blood mononuclear cells. Clinical and Experimental Immunology, 132(2), pp. 360-5.
Amel Kashipaz MR, et al. Normal Production of Inflammatory Cytokines in Chronic Fatigue and Fibromyalgia Syndromes Determined By Intracellular Cytokine Staining in Short-term Cultured Blood Mononuclear Cells. Clin Exp Immunol. 2003;132(2):360-5. PubMed PMID: 12699429.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Normal production of inflammatory cytokines in chronic fatigue and fibromyalgia syndromes determined by intracellular cytokine staining in short-term cultured blood mononuclear cells. AU - Amel Kashipaz,M R, AU - Swinden,D, AU - Todd,I, AU - Powell,R J, PY - 2003/4/18/pubmed PY - 2003/7/23/medline PY - 2003/4/18/entrez SP - 360 EP - 5 JF - Clinical and experimental immunology JO - Clin. Exp. Immunol. VL - 132 IS - 2 N2 - It has been proposed that cytokines play a role in the pathogenesis of chronic fatigue syndrome (CFS) and fibromyalgia syndrome (FMS). However, different studies have reported conflicting results using enzyme-linked immunosorbent assay or polymerase chain reaction to detect cytokines in these conditions. In the present study, for the first time, the production of inflammatory [interleukin (IL)-1alpha, IL-6, and TNF-alpha] and anti-inflammatory (IL-10) cytokines by CD14+ and CD14- peripheral blood mononuclear cells (PBMC) from chronic fatigue syndrome (CFS) and fibromyalgia syndrome (FMS) patients and sex- and age-matched normal subjects was investigated at the level of individual cells using the technique of intracellular cytokine staining and flow cytometry. Cultures were carried out in the presence of polymyxin B to inhibit the effect of endotoxins on cytokine production by monocytes. The mean intensity of fluorescence (MIF) and percentage of CD14+ (monocytes) and CD14- (lymphocytes) cytokine-producing mononuclear cells were comparable in patients and controls in either unstimulated or IFN-gamma-stimulated conditions. Our study indicates that dysregulation of cytokine production by circulating monocytes or non-monocytic cells (lymphocytes) is not a dominant factor in the pathogenesis of CFS/FMS. SN - 0009-9104 UR - https://www.unboundmedicine.com/medline/citation/12699429/Normal_production_of_inflammatory_cytokines_in_chronic_fatigue_and_fibromyalgia_syndromes_determined_by_intracellular_cytokine_staining_in_short_term_cultured_blood_mononuclear_cells_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0009-9104&date=2003&volume=132&issue=2&spage=360 DB - PRIME DP - Unbound Medicine ER -