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Molecular characterization of whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae) populations infesting cassava.
Bull Entomol Res. 2003 Apr; 93(2):97-106.BE

Abstract

Bemisia tabaci (Gennadius) populations, collected from cassava and other plants in major cassava-cultivation areas of Sub-saharan Africa and from elsewhere around the world, were studied to determine their biotype status and genetic variation. Random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) markers were used to examine the genetic structure of the populations. The dendogram obtained using the neighbour joining method (NJ) split the cassava-associated populations from the non-cassava types with a 100% bootstrap probability. Analysis of molecular variance (AMOVA) of the RAPD fragments revealed that 63.2% of the total variation was attributable to differences among populations, while the differences among groups (host) and within populations accounted for 27.1 and 9.8% respectively. Analysis of the internally transcribed spacer region I (ITS 1) of the ribosomal DNA confirmed that the cassava populations of B. tabaci populations were distinct from non-cassava populations. Experiments to establish whitefly populations on various host plants revealed that cassava-associated populations were restricted to cassava only, whereas B. tabaci from other hosts were polyphagous but did not colonize cassava. Hence, populations of B. tabaci from cassava in Africa represent a distinct group.

Authors+Show Affiliations

Department of Crop Protection and Environmental Biology, University of Ibadan, Nigeria.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

12699530

Citation

Abdullahi, I, et al. "Molecular Characterization of Whitefly, Bemisia Tabaci (Hemiptera: Aleyrodidae) Populations Infesting Cassava." Bulletin of Entomological Research, vol. 93, no. 2, 2003, pp. 97-106.
Abdullahi I, Winter S, Atiri GI, et al. Molecular characterization of whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae) populations infesting cassava. Bull Entomol Res. 2003;93(2):97-106.
Abdullahi, I., Winter, S., Atiri, G. I., & Thottappilly, G. (2003). Molecular characterization of whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae) populations infesting cassava. Bulletin of Entomological Research, 93(2), 97-106.
Abdullahi I, et al. Molecular Characterization of Whitefly, Bemisia Tabaci (Hemiptera: Aleyrodidae) Populations Infesting Cassava. Bull Entomol Res. 2003;93(2):97-106. PubMed PMID: 12699530.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular characterization of whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae) populations infesting cassava. AU - Abdullahi,I, AU - Winter,S, AU - Atiri,G I, AU - Thottappilly,G, PY - 2003/4/18/pubmed PY - 2003/6/17/medline PY - 2003/4/18/entrez SP - 97 EP - 106 JF - Bulletin of entomological research JO - Bull Entomol Res VL - 93 IS - 2 N2 - Bemisia tabaci (Gennadius) populations, collected from cassava and other plants in major cassava-cultivation areas of Sub-saharan Africa and from elsewhere around the world, were studied to determine their biotype status and genetic variation. Random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) markers were used to examine the genetic structure of the populations. The dendogram obtained using the neighbour joining method (NJ) split the cassava-associated populations from the non-cassava types with a 100% bootstrap probability. Analysis of molecular variance (AMOVA) of the RAPD fragments revealed that 63.2% of the total variation was attributable to differences among populations, while the differences among groups (host) and within populations accounted for 27.1 and 9.8% respectively. Analysis of the internally transcribed spacer region I (ITS 1) of the ribosomal DNA confirmed that the cassava populations of B. tabaci populations were distinct from non-cassava populations. Experiments to establish whitefly populations on various host plants revealed that cassava-associated populations were restricted to cassava only, whereas B. tabaci from other hosts were polyphagous but did not colonize cassava. Hence, populations of B. tabaci from cassava in Africa represent a distinct group. SN - 0007-4853 UR - https://www.unboundmedicine.com/medline/citation/12699530/Molecular_characterization_of_whitefly_Bemisia_tabaci__Hemiptera:_Aleyrodidae__populations_infesting_cassava_ DB - PRIME DP - Unbound Medicine ER -